Ukr.Biochem.J. 2014; Volume 86, Issue 4, Jul-Aug, pp. 61-68
doi: http://dx.doi.org/10.15407/ubj86.04.061
Influence of vermiculite particles on antioxidant properties of cultural medium of Bacillus subtilis IMV V-7023
I. O. Skorochod, I. K. Kurdish
Zabolotny Institute of Microbiology and Virology, National Academy of Sciences of Ukraine, Kyiv;
e-mail: Kurdish@serv.imv.kiev.ua
It is shown that in the process of cultivation of Bacillus subtilis IMV V-7023 in the medium with vermiculite (1.5–5.0 g/l) one can observe the oppressing of some indexes of antioxidant properties of cultural medium of bacteria. In particular, a decline of hydroxyl radical scavenging activity in the Fenton reaction by 2.8–11.6%, ability to inhibit formation of malondialdehyde – by 4.4–13.1% and inactivation of 2,2′-Diphenyl-1-picrylhydrazyl (DPPH·) radical – by 3.1–8.5% were observed. Thus oxidant activity increased substantially. Besides oppressing influence of particles of vermiculite on protector properties of the cultural medium of bacilli it is found out that with the increase of the content of dispersible material in the nutrient medium the reducing power of cultural medium of these bacteria increased.
Keywords: active oxygen species, antioxidant activity, Bacillus subtilis, free radicals, vermiculite
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