Ukr.Biochem.J. 2014; Volume 86, Issue 4, Jul-Aug, pp. 61-68


Influence of vermiculite particles on antioxidant properties of cultural medium of Bacillus subtilis IMV V-7023

I. O. Skorochod, I. K. Kurdish

Zabolotny Institute of Microbiology and Virology, National Academy of Sciences of Ukraine, Kyiv;

It is shown that in the process of cultivation of Bacillus subtilis IMV V-7023 in the medium with vermiculite (1.5–5.0 g/l) one can observe the oppressing of some indexes of antioxidant properties of cultural medium of bacteria. In particular,  a decline of hydroxyl radical scavenging activity in the Fenton reaction by 2.8–11.6%, ability to inhibit formation of malondialdehyde – by 4.4–13.1% and inactivation of 2,2′-Diphenyl-1-picrylhydrazyl (DPPH·) radical – by 3.1–8.5% were observed. Thus oxidant activity increased substantially. Besides oppressing influence of particles of vermiculite on protector properties of the cultural medium of bacilli it is found out that with the increase of the content of dispersible material in the nutrient medium the reducing power of cultural medium of these bacteria increased.

Keywords: , , , ,


  1. Kurdish IK. Introduction of microorganisms in the agroecosystems. Kyiv: Naukova dymka, 2010. 253 p. (In Ukrainian).
  2. Kurdish IK. Granulated microbial preparations for plant cultivation: Theory and Practice. Kiev: KVITs, 2001. 141 p. (In Russian).
  3. Graham-Weiss L, Bennet LM, Paau AS. Production of bacterial inoculants by direct fermentation on nutrient-supplemented vermi­culite. Appl Environ Microbiol. 1987 Sep;53(9):2138-41. PubMed, PubMedCentral
  4. Laktionov YV, Kozhemyakov AP, Popova TA. Creation of new drug forms of symbiotic and associative rhizobacteria with an improved efficiency. Materials of the V All-Russian congress of the soil scientists the name of V. V. Dokuchaev. State publishing house, Rostov-on-Don, 18-23 August, 2008. 557 p. (In Russian).
  5. Pat. № 54923 A. Bacillus subtilis strain for the receipt of bacterial preparation for a plant growing. Kurdish I. K., Roy A. O. Publ. 17.03.2003, Bul. № 3. (In Ukrainian).
  6. Roy AA, Bulavenko LV, Kurdish IK. New strains of soil bacilli which mineralize organic phosphorus compounds. Mikrobiol Zhurn. 2001 Jul-Aug;63(4):9-14. Russian.  PubMed
  7. Roy AA, Zaloilo OV, Chernova LS, Kurdish IK. Antagonistic activity of phosphate-mobilizing bacilli to phytopathogenic mushrooms and bacteria. Agroekol Zh. 2005;(1):50–55. (In Russian).
  8. Skorochod IO, Tserkovniak LS, Kurdish IK, Plotnikov VV, Gylchuk VG, Korniychuk OV. Influence of granulated bacterial preparation complex action on the growth and yield of barley.  Mikrobiol Zhurn. 2012 May-Jun;74(3):23-8. Ukrainian. PubMed
  9. Skorokhod IO, Tserkovniak LS, Kudrysh IK. The antioxidant effect of Bacillus subtilis and Azotobacter vinelandii on the seeds of crops. Mikrobiol Zhurn. 2011 Jan-Feb;73(1):44-50. Ukrainian.  PubMed
  10. Spizizen J. Transformation of biochemically deficient strains of Bacillus subtilis by deoxyribonucleate. Proc Natl Acad Sci USA. 1958 Oct 15;44(10):1072-8.  PubMed, PubMedCentral, CrossRef
  11. Smirnoff N, Cumbes QJ. Hydroxyl radical scavenging activity of compatible solutes. Phytochemistry. 1989;28(4):1057–1060.  CrossRef
  12. Oyaizu M. Studies on product of browning reaction prepared from glucose amine. Jpn J Nutr. 1986;44(6):307–315. CrossRef
  13. Galaktionova LP, Molchanov AV, Yelchani­nova SA, Varshavsky BYa. Lipid peroxidation in patients with gastroduodenal ulcer. Clin Lab Diagnostics. 1998;(6):10–14. (In Russian).
  14. Chevari S, Andyal T, Shtrenger Y. Determination of the antioxidant properties of the blood and their diagnostic significance in the elderly. Lab Delo. 1991;(10):9-13. Russian.  PubMed
  15. Shimada K, Fujikawa K, Yahara K, Nakamura T. Antioxidative properties of xanthin on autoxidation of soybean oil in cyclodextrin emulsion. J Agric Food Chem. 1992;40(6):945–948.  CrossRef
  16. National standard of Ukraine. Seed of agricultural cultures. Methods of determination of quality. DSTY 4138-2002. (In Ukrainian).
  17. Lakin G. F. Biometria. Moscow: High School, 1968. 352 p. (In Russian).
  18. Zimon A. D. Entertaining colloid chemistry. Moscow: Agar, 2002. 168 p. (In Russian).
  19. Nel A, Xia T, Mädler L, Li N. Toxic potential of materials at the nanolevel. Science. 2006 Feb 3;311(5761):622-7. Review. PubMed, CrossRef
  20. Wu CR, Huang MY, Lin YT, Ju Y, Ching H. Antioxidant properties of Cortex Fraxini and its simple coumarins. Food. Chem. 2007;104(4):1464–1471. CrossRef
  21. Miller NJ, Sampson J, Candeias LP, Bramley PM, Rice-Evans CA. Antioxidant activities of carotenes and xanthophylls. FEBS Lett. 1996 Apr 22;384(3):240-2.
    PubMed, CrossRef
  22. May JJ, Wendrich TM, Marahiel MA. The dhb operon of Bacillus subtilis encodes the biosynthetic template for the catecholic siderophore 2,3-dihydroxybenzoate-glycine-threonine trimeric ester bacillibactin.  J Biol Chem. 2001 Mar 9;276(10):7209-17. PubMed, CrossRef
  23. Fischer HC, Chan WC. Nanotoxicity: the growing need for in vivo study. Curr Opin Biotechnol. 2007 Dec;18(6):565-71. Review.  PubMed, CrossRef
  24. Nazarov D. Si-nanokrystals against cancer. In the science world. 2012;(8):4-9. (In Russian).
  25. Sigler K, Chaloupka J, Brozmanová J, Stadler N, Höfer M. Oxidative stress in microorganisms–I. Microbial vs. higher cells–damage and defenses in relation to cell aging and death.  Folia Microbiol (Praha). 1999;44(6):587-624. Review. PubMed, CrossRef
  26. Tserkovniak LS, Kurdish IK. Phosphate-mobilizing bacteria Bacillus subtilis as phenolic producers. Appl Biochem Microbiol. 2009;45(3):279–284. (In Russian).  CrossRef
  27. Burlakova EB, Gubareva AE, Arkhipva GV, Roginskiy VA. Modulation of lipid peroxidation by biogenic amines in model systems. Vopr Med Khim. 1992 Mar-Apr;38(2):17-20. Russian. PubMed
  28. Basaga HS. Biochemical aspects of free radicals. Biochem Cell Biol. 1990 Jul-Aug;68(7-8):989-98. Review.  PubMed, CrossRef

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