Tag Archives: glial fibrillary acid protein

Astrocyte specific proteins content in the different parts of the rat and mongolian gerbil brain during ontogenesis

Y. P. Kovalchuk*, H. N. Shiyntum, G. A. Ushakovа

Oles Honchar Dniprо National University, Ukraine;
*e-mail: yulka.5868152@ukr.net

Received: 28 October 2021; Accepted: 17 May 2021

Astrocyte-specific proteins are used as markers of astrocytes, particularly in the case of age-related brain dysfunctions and neurodegenerative disorders. However, the data on the content of these proteins in different parts of the brain during ontogenesis are insufficient. In this research, the content of astrocyte specific Ca2+-binding protein S100B and glial fibrillary acidic protein (GFAP) in cerebellum, thalamus, and hippocampus of Wistar rats and Mongolian gerbils during ontogenesis were determined. Animals were divided by age into four groups (n = 6-10): 1 – newborn (1-day-old); 2 – 30-day-old; 3 – 90-day-old; 4 – 180-day-old. Fractions of soluble and filamentous proteins from the different parts of the brain were obtained by differential centrifugation and solubilization with 4M urea. The quantitative levels of S100B and GFAP were determined with ELISA. It was revealed that the content of S100B protein increased significantly by day 180 in all studied parts of the rat and gerbil brain. The content of GFAP both soluble and filamentous forms in the brain of 1 day-old animals was low, but raised significantly after 30 days in all studied parts of gerbil brain, and increased gradually up to 180 days in the rat brain. The magnitude of the increase in the content of the studied astrocyte-specific proteins was shown to be different and to depend on the brain part, postnatal development stage of the animals and their species.

Distribution of glial fibrillary acidic protein in different parts of the rat brain under cadmium exposure

Yu. P. Kovalchuk1, I. V. Prischepa1, U. Si2, V. S. Nedzvetsky1,
Y. G. Kot2, E. E. Persky2, G. A. Ushakova1

1Oles’ Honchar Dnepropetrovsk National University, Ukraine;
2V. N. Karazin  Kharkiv National University, Ukraine;
e-mail:  yulka.kovalchuk.5868152@mail.ru

The chronic effects of low doses of cadmium on the distribution of soluble and filament forms of glial fibrillary acidic protein (GFAP) and their polypeptide fragments in different parts of the rat brain were investigated. Obtained results showed dose-dependent effect of cadmium on the soluble form of GFAP and more pronounced effect on the filament form and composition of the polypeptide fragments of the protein in the rat brain. Prolonged intoxication by cadmium ions in a dose of 1.0 µg/kg of body weight induced a significant decrease in soluble GFAP and an increase in the filament form in the rat brain, pointing to the development of reactive astrogliosis and the risk of neurodegeneration.