Ukr.Biochem.J. 2021; Volume 93, Issue 3, May-Jun, pp. 13-23
doi: https://doi.org/10.15407/ubj93.03.013
Astrocyte specific proteins content in the different parts of the rat and mongolian gerbil brain during ontogenesis
Y. P. Kovalchuk*, H. N. Shiyntum, G. A. Ushakovа
Oles Honchar Dniprо National University, Ukraine;
*e-mail: yulka.5868152@ukr.net
Received: 28 October 2021; Accepted: 17 May 2021
Astrocyte-specific proteins are used as markers of astrocytes, particularly in the case of age-related brain dysfunctions and neurodegenerative disorders. However, the data on the content of these proteins in different parts of the brain during ontogenesis are insufficient. In this research, the content of astrocyte specific Ca2+-binding protein S100B and glial fibrillary acidic protein (GFAP) in cerebellum, thalamus, and hippocampus of Wistar rats and Mongolian gerbils during ontogenesis were determined. Animals were divided by age into four groups (n = 6-10): 1 – newborn (1-day-old); 2 – 30-day-old; 3 – 90-day-old; 4 – 180-day-old. Fractions of soluble and filamentous proteins from the different parts of the brain were obtained by differential centrifugation and solubilization with 4M urea. The quantitative levels of S100B and GFAP were determined with ELISA. It was revealed that the content of S100B protein increased significantly by day 180 in all studied parts of the rat and gerbil brain. The content of GFAP both soluble and filamentous forms in the brain of 1 day-old animals was low, but raised significantly after 30 days in all studied parts of gerbil brain, and increased gradually up to 180 days in the rat brain. The magnitude of the increase in the content of the studied astrocyte-specific proteins was shown to be different and to depend on the brain part, postnatal development stage of the animals and their species.
Keywords: cerebellum, glial fibrillary acid protein, hippocampus, ontogenesis, S100b, thalamus
References:
- Chandrasekaran A, Avci HX, Leist M, Kobolák J, Dinnyés A. Astrocyte Differentiation of Human Pluripotent Stem Cells: New Tools for Neurological Disorder Research. Front Cell Neurosci. 2016;10:215. PubMed, PubMedCentral, CrossRef
- Gigase FAJ, Snijders GJLJ, Boks MP, de Witte LD. Neurons and glial cells in bipolar disorder: A systematic review of postmortem brain studies of cell number and size. Neurosci Biobehav Rev. 2019;103:150-162. PubMed, CrossRef
- Phatnani H, Maniatis T. Astrocytes in neurodegenerative disease. Cold Spring Harb Perspect Biol. 2015;7(6):a020628. PubMed, PubMedCentral, CrossRef
- Galland F, Seady M, Taday J, Smaili SS, Gonçalves CA, Leite MC. Astrocyte culture models: Molecular and function characterization of primary culture, immortalized astrocytes and C6 glioma cells. Neurochem Int. 2019;131:104538. PubMed, CrossRef
- Morioka N, Zhang FF, Nakamura Y, Kitamura T, Hisaoka-Nakashima K, Nakata Y. Tumor necrosis factor-mediated downregulation of spinal astrocytic connexin43 leads to increased glutamatergic neurotransmission and neuropathic pain in mice. Brain Behav Immun. 2015;49:293-310. PubMed, CrossRef
- Bosworth AP, Allen NJ. The diverse actions of astrocytes during synaptic development. Curr Opin Neurobiol. 2017;47:38-43. PubMed, CrossRef
- Fleischer W, Theiss S, Slotta J, Holland C, Schnitzler A. High-frequency voltage oscillations in cultured astrocytes. Physiol Rep. 2015;3(5):e12400. PubMed, PubMedCentral, CrossRef
- Fernandez-Fernandez S, Almeida A, Bolaños JP. Antioxidant and bioenergetic coupling between neurons and astrocytes. Biochem J. 2012;443(1):3-11. PubMed, CrossRef
- Verkhratsky A, Nedergaard M. Physiology of Astroglia. Physiol Rev. 2018;98(1):239-389. PubMed, PubMedCentral, CrossRef
- Liang Y, Qiu Y , Du J, Liu J, Fang J, Zhu J, Fang J. Inhibition of spinal microglia and astrocytes contributes to the anti-allodynic effect of electroacupuncture in neuropathic pain induced by spinal nerve ligation. Acupunct Med. 2016;34(1):40-47. PubMed, CrossRef
- Kuter K, Olech Ł, Głowacka U. Prolonged Dysfunction of Astrocytes and Activation of Microglia Accelerate Degeneration of Dopaminergic Neurons in the Rat Substantia Nigra and Block Compensation of Early Motor Dysfunction Induced by 6-OHDA. Mol Neurobiol. 2018;55(4):3049-3066. PubMed, PubMedCentral, CrossRef
- Morita M, Ikeshima-Kataoka H, Kreft M, Vardjan N, Zorec R, Noda M. Metabolic Plasticity of Astrocytes and Aging of the Brain. Int J Mol Sci. 2019;20(4):941. PubMed, PubMedCentral, CrossRef
- Wilhelmsson U, Pozo-Rodrigalvarez A, Kalm M, de Pablo Y, Widestrand Å, Pekna M, Pekny M. The role of GFAP and vimentin in learning and memory. Biol Chem. 2019;400(9):1147-1156. PubMed, CrossRef
- Tykhomyrov AA, Pavlova AS, Nedzvetsky VS. Glial fibrillary acidic protein (GFAP): on the 45th anniversary of its discovery. Neurophysiology. 2016; 48(1): 54-71. CrossRef
- Pfuhlmann K, Schriever SC, Legutko B, Baumann P, Harrison L, Kabra DG, Baumgart EV, Tschöp MH, Garcia-Caceres C, Pfluger PT. Calcineurin A beta deficiency ameliorates HFD-induced hypothalamic astrocytosis in mice. J Neuroinflammation. 2018;15(1):35. PubMed, PubMedCentral, CrossRef
- O’Callaghan JP, Sriram K. Glial fibrillary acidic protein and related glial proteins as biomarkers of neurotoxicity. Expert Opin Drug Saf. 2005;4(3):433-442. PubMed, CrossRef
- Brenner M. Role of GFAP in CNS injuries. Neurosci Lett. 2014;565:7-13. PubMed, PubMedCentral, CrossRef
- Pierozan P, Zamoner A, Soska ÂK, de Lima BO, Reis KP, Zamboni F, Wajner M, Pessoa-Pureur R. Signaling mechanisms downstream of quinolinic acid targeting the cytoskeleton of rat striatal neurons and astrocytes. Exp Neurol. 2012;233(1):391-399. PubMed, CrossRef
- Garbuglia M, Verzini M, Sorci G, Bianchi R, Giambanco I, Agneletti AL, Donato R. The calcium-modulated proteins, S100A1 and S100B, as potential regulators of the dynamics of type III intermediate filaments. Braz J Med Biol Res. 1999;32(10):1177-1185. PubMed, CrossRef
- Beharier O, Kahn J, Shusterman E, Sheiner E. S100B – a potential biomarker for early detection of neonatal brain damage following asphyxia. J Matern Fetal Neonatal Med. 2012;25(9):1523-1528. PubMed, CrossRef
- Medical ethics and human rights: provisions on the use of animals in biomedical experiments. Exp Clin Physiol Biochem. 2003; 22(2): 108–109. (In Ukrainian).
- Fomenko OZ, Ushakova GO, Pierzynowski SG. Proteins of astroglia in the rat brain under experimental chronic hepatitis and 2-oxoglutarate effect. Ukr Biokhim Zhurn. 2011;83(1):69-76. PubMed
- Bradford MM. A rapid and sensitive method for the quantitation of microgram quantities of protein utilizing the principle of protein-dye binding. Anal Biochem. 1976;72(1-2):248-254. PubMed, CrossRef
- Ngo TT, Lenhoff GM, Yaklich A. ELISA. Moscow: Mir, 1998. 444 p. (In Russian).
- Liutenko MA. Morphological features of the development of the cerebellum of nonbred white rats at the stage of early ontogenesis. SCI-ARTICLE.RU. 2014;(9):161-164. (In Russian).
- Kruijff S, Hoekstra HJ. The current status of S-100B as a biomarker in melanoma. Eur J Surg Oncol. 2012;38(4):281-285. PubMed, CrossRef
- Nedzvetsky VS, Nerush PA. Cognitive deficiency and imbalance of expression of neurospecific proteins in streptozotocin-induced diabetes. Neurosci Theor Clin Aspect. 2005; 1(1): 35-38. (In Russian).
- Linnemann D, Skarsfelt T. Regional changes in expression of NCAM, GFAP, and S100 in aging rat brain. Neurobiol Aging. 1994;15(5):651-655. PubMed, CrossRef
- Sheng JG, Mrak RE, Rovnaghi CR, Kozlowska E, Van Eldik LJ, Griffin WS. Human brain S100 beta and S100 beta mRNA expression increases with age: pathogenic implications for Alzheimer’s disease. Neurobiol Aging. 1996;17(3):359-363. PubMed, CrossRef
- Wu Y, Zhang AQ, Yew DT. Age related changes of various markers of astrocytes in senescence-accelerated mice hippocampus. Neurochem Int. 2005;46(7):565-574. PubMed, CrossRef
- Peskind ER, Griffin WS, Akama KT, Raskind MA, Van Eldik LJ.Cerebrospinal fluid S100B is elevated in the earlier stages of Alzheimer’s disease. Neurochem Int. 2001;39(5-6):409-413. PubMed, CrossRef
- Massaro AN, Chang T, Kadom N, Tsuchida T, Scafidi J, Glass P, McCarter R, Baumgart S, Vezina G, Nelson KB. Biomarkers of brain injury in neonatal encephalopathy treated with hypothermia. J Pediatr. 2012;161(3):434-440. PubMed, PubMedCentral, CrossRef
- Yang Z, Wang KKW. Glial fibrillary acidic protein: from intermediate filament assembly and gliosis to neurobiomarker. Trends Neurosci. 2015;38(6):364-374. PubMed, PubMedCentral, CrossRef
- Bramanti V, Tomassoni D, Avitabile M, Amenta F, Avola R. Biomarkers of glial cell proliferation and differentiation in culture. Front Biosci (Schol Ed). 2010;2(2):558-570. PubMed, CrossRef
- Gómez-Gonzalo M, Martin-Fernandez M, Martínez-Murillo R, Mederos S, Hernández-Vivanco A, Jamison S, Fernandez AP, Serrano J, Calero P, Futch HS, Corpas R, Sanfeliu C, Perea G, Araque A. Neuron-astrocyte signaling is preserved in the aging brain. Glia. 2017;65(4):569-580. PubMed, PubMedCentral, CrossRef
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