Tag Archives: homocysteine

Indicators of mineral metabolism and dental status of young rats born from female with methionine-induced hyperhomocysteinemia

O. Kutelmakh1*, R. Lesyk2,3, Yu. Chumakova4,
S. Bondar1, V. Dronenko1, L. Shkilniak1

1Vinnytsia National Pirogov Memorial Medical University, Vinnytsya, Ukraine;
2Danylo Halytsky Lviv National Medical University, Lviv, Ukraine;
3University of Information Technology and Management in Rzeszow, Rzeszow, Poland;
4International Humanitarian University, Odessa, Ukraine;
*e-mail: ahzelo1968vin@i.ua

Received: 12 July 2021; Accepted: 12 November 2021

We assume that the violation of homocysteine (Hcy) metabolism and vitamin D level in mothers’ utero causes various abnormalities in offspring, including hard-woven teeth and periodontium impairments. The aim of this study was to evaluate the biochemical parameters of blood and alveolar bone homogenates and assess dental status of offspring born from female rats under long-term methionine hyperhomocysteinemia (HHcy). Clinical, biochemical and morphometric studies were performed on 30 female rats and 281 infantile and early juvenile rats. Serum Hcy levels were determined by competitive immunoassay with electrochemiluminescent detection, 25-hydroxyvitamin D (25OHD) level was estimated  by ELISA. No violation of Hcy serum level was found in rats born from females with long-term methionine-induced hyperhomocysteinaemia. Hyperhomocysteinaemic diet caused by long-term use of methionine reduced the level of total and ionized calcium and phosphates in the blood of pregnant females and their offspring. Elevated levels of homocysteine and decreased vitamin D levels in pregnant females caused a delay in the complete physiological eruption of permanent molars in their offspring. Biochemical changes in the blood, jawbones and the presence of early carious processes in the teeth of young rats, in our opinion, are the result of metabolic disorders in utero caused by hyperhomocysteinaemia and vitamin D deficiency in their mothers.

Some biochemical changes in patients with acute ischemic stroke

O. Ja. Mykhalojko, І. Ja. Mykhalojko

Ivano-Frankivsk National Medical University, Ukraine;
e-mail: myhalojko@i.ua

The aim was to determine the alterations in levels of vasculair endothelial growth factor (VEGF), homocysteine (HC) and lipid profile in patients with acute ischemic stroke (AIS), to establish the relationship between them. A clinical and neurological examination of 120 patients aged 56 to 75 years with AIS was done. Diagnosis was established taking into account results of spiral computer and magnetic resonance tomography. The control group included 20 people with no signs of acute cerebrovascular and severe somatic pathology aged 59.35±1.8 years. Assessment of disability was performed at admission and during the treatment using the scale NIHSS. In all relevant patients in 1-2 days after stroke, the concentration of HC and VEGF levels was determined by ELISA. Blood lipid profile was determined by enzymatic cоlorimetric method. The study found that increasing concentration of plasma HC is associated with intensity of hyperlipidemia, and hyperhomocysteinemia severity prevails over the changes in the lipid profile components. During AIS a significant increase in the level of VEGF in the blood was observed, indicating the presence of vascular accident and activation of angiogenesis. Severity of vascular endothelial lesions is associated with the degree of increasing concentration VEGF that appears most significant in patients with severe AIS.

Stoichiometric model of folate-dependent metabolism of one-carbon units in human placenta

R. R. Rodriguez1, I. S. Lushchyk2, M. Yu. Obolenska1

 1Institute of Molecular Biology and Genetics, National Academy of Sciences of Ukraine, Kyiv;
e-mail: northernwizard@yandex.ua;
2National University Kyiv-Mohyla Academy, Ukraine

The work is dedicated to creation of the mathematical model of folate-dependent one-carbon unit metabolism (FOCM) and study of its function in human placenta under homocysteine load and the most common mutations in the genes of methylenetetrahydrofolate reductase (MTHFR) and cystathionine β-synthase (CBS). In the model we have taken into account specific features of placental expression of genes that encode enzymes of FOCM. Using software tools Metatool and COBRAToolbox­ we have identified key metabolites, elementary modes and metabolic fluxes through different reactions of the system. It is shown that the most vulnerable links in the system are the folate cycle and synthesis of precursors of nucleic acids, inosine monophosphate and thymidyne monophosphates, which are changing in the broad range from significant inhibition to activation depending on the imposed conditions. The most stable links in the system are the reactions of glutathione and taurine synthesis. Simulation results coincide with the results obtained in similar experimental conditions. Under certain imposed conditions non-obvious relationships between the system links are revealed, and this becomes the basis for a purposeful test of predictions generated by the model.

Cardiovascular disease among patients with type 2 diabetes: role of homocysteine as an inflammatory marker

Ashok Sahu1,2, Trapti Gupta2, Arvind Kavishwar3, R. K. Singh4

1MGM Medical College Indore, Jabalpur, India;
e-mail: asahu888@gmail.com; asahu888@yahoo.co.in;
2NSCB Medical College, Jabalpur, India;
3Regional Malaria Research Centre of Tribal (ICMR), Jabalpur, India;
4Chirayu Hospital, Bhopal, India

It is known that inflammation has a role in the pathogenesis of cardiovascular diseases; measurement of inflammatory markers improves the risk prediction of cardiovascular diseases. Hyperhomocysteinemia has been correlated with the occurrence of blood clots, heart attacks and strokes; though it is unclear whether hyperhomocysteinemia is an independent risk factor for these conditions. In the present study, we aimed to evaluate the role of homocysteine in type 2 diabetes patients with cardiovascular disease in a population of Madhya Pradesh India. Total 100 type 2 diabetes patients were included in the study, of these 50 had angiographically proven cardiovascular disease and 50 had no evidence of it. High sensitivity C-reactive protein, fibrinogen, and lipoprotein (a) were measured in serum. Homocysteine, blood glucose in plasma, erythrocyte sedimentation rate, glycated haemoglobin were measured in whole blood. A albumin excretion rate, creatinine clearance rate were measured in the urine sample for renal function. It was shown that, levels of homocysteine and other inflammatory markers were elevated significantly in the group II (n = 50). A correlation between hyperhomocysteinemia and inflammatory markers in patients with impaired renal function was observed. It was concluded that impairment of renal function is a key factor that affects homocysteine level.