Tag Archives: kinetics of interaction

Kinetics of interaction between polyreactive immunoglobulins and antigen

S. A. Bobrovnik1*, O. V. Ogloblya2, M. O. Demchenko1, S. V. Komisarenko1

1Palladin Institute of Biochemistry, National Academy of Sciences of Ukraine, Kyiv;
2ESC “Institute of Biology and Medicine”, Taras Shevchenko National University of Kyiv, Ukraine;
*e-mail: s-bobrov@ukr.net

Received: 28 January 2020; Accepted: 25 June 2020

A number of experimental kinetics curves of mice polyreactive immunoglobulins (PRIGs) binding to ovalbumin immobilized on immunologic plates were obtained at different temperatures. Analysis of these curves allowed us to conclude that the model of PRIGs interaction with antigens proposed by us earlier and consisted on PRIGs activation (i.e. exposition of hydrophobic patches on PRIGs surface) and either sequential binding to antigen or  inactivation was is in a good agreement with the experimental data obtained in this study. We have designed a method of the rate constants evaluation from experimental binding curves. It was found  that the rate constant of the activated PRIGs binding to immobilized antigen did not depend on temperature. The rate constant of PRIGs activation occurred to be depend on temperature more strongly than the rate constant of PRIGs inactivation. We have concluded from the acquired dependences that at 37°С the number of activated PRIGs was 15 times higher than that at 0°С.

Kinetics of interaction between polyreactive immunoglobulins and antigen. The theory

S. A. Bobrovnik, M. O. Demchenko, S. V. Komisarenko

Palladin Institute of Biochemistry, National Academy of Sciences of Ukraine, Kyiv;
e-mail: s-bobrov@ukr.net

Received: 14 February 2019; Accepted: 17 May 2019

Dynamics of association between polyreactive immunoglobulins (PRIGs) and immobilized antigens is considered on the base of our model of PRIGs-antigen interaction, which was suggested by us earlier. This process of PRIGs binding to an immobilized antigen was described  with a system of differential equations. The solution of this system of differential equations gives mathematical expressions that relate the dynamics of the reactant concentrations and time of the reaction. Using Microsoft Excel program the theoretical curves were calculated and plotted that described the dynamics of “active”, “nonactive” PRIGs in solution as well as PRIGs that were bound to an immobilized antigen. Conclusions drawn by us earlier about very high dependen­ce of reaction PRIGs with an antigen from temperature were confirmed.