Ukr.Biochem.J. 2020; Volume 92, Issue 5, Sep-Oct, pp. 15-22


Kinetics of interaction between polyreactive immunoglobulins and antigen

S. A. Bobrovnik1*, O. V. Ogloblya2, M. O. Demchenko1, S. V. Komisarenko1

1Palladin Institute of Biochemistry, National Academy of Sciences of Ukraine, Kyiv;
2ESC “Institute of Biology and Medicine”, Taras Shevchenko National University of Kyiv, Ukraine;

Received: 28 January 2020; Accepted: 25 June 2020

A number of experimental kinetics curves of mice polyreactive immunoglobulins (PRIGs) binding to ovalbumin immobilized on immunologic plates were obtained at different temperatures. Analysis of these curves allowed us to conclude that the model of PRIGs interaction with antigens proposed by us earlier and consisted on PRIGs activation (i.e. exposition of hydrophobic patches on PRIGs surface) and either sequential binding to antigen or  inactivation was is in a good agreement with the experimental data obtained in this study. We have designed a method of the rate constants evaluation from experimental binding curves. It was found  that the rate constant of the activated PRIGs binding to immobilized antigen did not depend on temperature. The rate constant of PRIGs activation occurred to be depend on temperature more strongly than the rate constant of PRIGs inactivation. We have concluded from the acquired dependences that at 37°С the number of activated PRIGs was 15 times higher than that at 0°С.

Keywords: , , ,


  1. Bobrovnik SA, Demchenko MO, Komisarenko SV. Kinetics of interaction between polyreactive immunoglobulins and antigen. The theory. Ukr Biochem J.  2019;  91(4); 88-94.  CrossRef
  2. Bobrovnik SA. Polyreactive immunoglobulins: molecular properties and functions. Comments Mol Cell Biophys. 1999; 9; 323-356.
  3. Gunti S, Herman SEM, Gottumukkala RVSRK, Xiong Y, Sun C, Carmona GN, Wiestner A, Notkins AL. Polyreactive antibodies in CLL correlate with the level of immunoglobulins not the number of B lymphocytes. Leuk Lymphoma. 2019;60(1):242-245. PubMed, PubMedCentral, CrossRef
  4. Liao H, Zhang Z. Polyreactive Antibodies in Anti-HIV-1 Responses. Curr Mol Med. 2018;18(2):126-133. PubMed, CrossRef
  5. Ravindranath MH. HLA Class Ia and Ib Polyreactive Anti-HLA-E IgG2a Monoclonal Antibodies (TFL-006 and TFL-007) Suppress Anti-HLA IgG Production by CD19+ B Cells and Proliferation of CD4+ T Cells While Upregulating Tregs. J Immunol Res. 2017;2017:3475926. PubMed, PubMedCentral, CrossRef
  6. Bunker JJ, Erickson SA, Flynn TM, Henry C, Koval JC, Meisel M, Jabri B, Antonopoulos DA, Wilson PC, Bendelac A. Natural polyreactive IgA antibodies coat the intestinal microbiota. Science. 2017;358(6361):eaan6619. PubMed, PubMedCentral, CrossRef
  7. Liao H, Yu Y, Li S, Yue Y, Tao C, Su K, Zhang Z. Circulating Plasmablasts from Chronically Human Immunodeficiency Virus-Infected Individuals Predominantly Produce Polyreactive/Autoreactive Antibodies. Front Immunol. 2017;8:1691. PubMed, PubMedCentral, CrossRef
  8. Zorn E, See SB. Polyreactive natural antibodies in transplantation. Curr Opin Organ Transplant. 2017;22(1):8-13. PubMed, CrossRef
  9. Lecerf M, Jarossay A, Kaveri SV, Lacroix-Desmazes S, Dimitrov JD. Methods for Posttranslational Induction of Polyreactivity of Antibodies. Methods Mol Biol. 2017;1643:135-145. PubMed, CrossRef
  10. Gunti S , Notkins AL. Polyreactive Antibodies: Function and Quantification. J Infect Dis. 2015;212 Suppl 1(Suppl 1):S42-S46. PubMed, PubMedCentral, CrossRef
  11. Dimitrov JD, Planchais C, Roumenina LT, Vassilev TL, Kaveri SV, Lacroix-Desmazes  S. Antibody polyreactivity in health and disease: statu variabilis. J Immunol. 2013;191(3):993-999. PubMedCrossRef
  12. Mahendra A, Gangadharan B, André S, Boudjoghra M, Davi F, Lecerf M, Planchais C, Kaveri SV, Lacroix-Desmazes S, Dimitrov JD. Cryptic polyreactivity of IgG expressed by splenic marginal zone B-cell lymphoma. Mol Immunol. 2014;60(1):54-61. PubMed, CrossRef
  13. Bobrovnik SA. Polyreactive immunoglobulins recognize hydrophobic parts of proteins. Ukr Biokhim Zhurn. 2001;73(2):116-122. (In Russian).  PubMed
  14. Bobrovnik  SA, Marinets AV. Properties of polyreactive immunoglobulins.  Ukr Biokhim Zhurn. 1993;65(5):21-26. (In Russian). PubMed
  15. Bobrovnik SA. Determination the rate constants of some biexponential reactions. J Biochem Biophys Methods. 2000;42(1-2):49-63. PubMed, CrossRef

Creative CommonsThis work is licensed under a Creative Commons Attribution 4.0 International License.