Tag Archives: lactate
C(60) fullerene effect on the functional activity of rat gastrocnemius muscle during its regeneration after the open injury
D. M. Nozdrenko1, O. O. Gonchar2, N. E. Nurishchenko1,
V. O. Stetska1, T. Yu. Matviienko1, Ya. V. Stepanyuk3,
K. I. Bogutska1, Yu. I. Prylutskyy1*
1ESC “Institute of Biology and Medicine”, Taras Shevchenko National University of Kyiv, Ukraine;
2Bogomoletz Institute of Physiology, National Academy of Sciences of Ukraine, Kyiv;
3Medical Faculty, Lesya Ukrainka Volyn National University, Lutsk, Ukraine;
*e-mail: prylut@ukr.net
Received: 05 September 2024; Revised: 14 October 2024;
Accepted: 21 November 2024; Available on-line: 17 December 2024
Open injuries are one of the most common skeletal muscle traumas. The study aimed to estimate the effect of the oral administration of C60 fullerene aqueous solution (C60FAS) daily at a dose of 1 mg/kg on the restoration of rat skeletal muscle functional activity on the 5th, 10th and 15th day after the open trauma. Male Wistar rats were randomly divided into three groups of 12 animals in each: control, with muscle injury and with muscle injury+C60FAS. The isolated gastrocnemius muscle was subjected to open injury by transverse dissection with a depth of 1 mm. Stimulation of muscle efferents was carried out by electrical impulses generated using a strain gauge generator. The content of C-reactive protein, creatinine, lactate, reduced glutathione and the activity of catalase and superoxide dismutase in the rat blood were determined. According to the data obtained, application of C60FAS promotes the restoration of the functional activity of injured muscle, which was confirmed by a significant increase in gastrocnemius muscle force impulse, attenuation of the inflammatory and development of fatigue and normalization of pro- and antioxidant balance in the process of regeneration.
Changes in gene expression of lactate carriers (MCT1 and CD147) in cardiac muscle of diabetic male rats: the effect of dichloroacetate and endurance training
H. Rezaeinasab1*, A. Habibi1, M. Nikbakht1, M. Rashno2,3, S. Shakerian1
1Department of Exercise Physiology, Faculty of Sport Sciences, Shahid Chamran University of Ahvaz, Ahvaz, Iran;
2Department of Immunology, Faculty of Medicine, Ahvaz Jundishapur University of Medical Sciences, Ahvaz, Iran;
3Department of Cellular and Molecular Research Center, Ahvaz Jundishapur University of Medical Sciences, Ahvaz, Iran.
e-mail: hamed.rezaei2020@gmail.com
Received: 23 March 2020; Accepted: 25 June 2020
Lactate accumulation can activate the pathways of mitochondrial biogenesis in the heart muscle. The purpose of this study was to investigate the effects of Pyruvate Dehydrogenase Kinase 4 (PDK4) inhibition and endurance training on the gene expression of lactate carriers (MCT1 and CD147) in the cardiac muscle of STZ-diabetic rats. In this experimental study, 64 male Wistar rats were selected and randomly divided into eight groups after induction of diabetes with streptozotocin (STZ). The endurance training protocol was performed on a treadmill for 6 weeks. Intraperitoneal injection of DCA of 50 mg/ kg body weight was used for the inhibition of PDK4 in the myocardium. Gene expression were measured using real-time PCR. The two-way ANOVA test was used to analyze the data. The results of the study showed that after endurance training, the expression of MCT1, PDK4, and CD147 genes increased significantly in line with each other (P < 0.05), and by inhibition of PDK4 in the heart muscle, the expression of MCT1 and CD147 genes in the endurance training group + diabetes + DCA and in the diabetes group + DCA decreased significantly (P < 0.05). According to the results of this study, it can be concluded that the repeated accumulation of lactate caused by exercise training in diabetic patients decrease through mitochondrial adaptation by DCA injection and subsequently oxidative stress can be reduced in cardiac tissue of diabetic patients and heart efficacy can be increased.