Tag Archives: lactate dehydrogenase

Biochemical parameters of blood and tissue of the gastrocnemius muscle in chronically alcoholized rats under oral administration of C(60) fullerene aqueous solution

O. Motuziuk1,2, D. Nozdrenko2, S. Prylutska3,
K. Bogutska2, O. Korotkyi2, Yu. Prylutskyy2*

1Lesya Ukrainka Volyn National University, Lutsk, Ukraine;
2Taras Shevchenko National University of Kyiv, Ukraine;
*e-mail: prylut@ukr.net;
3National University of Life and Environmental Science of Ukraine, Kyiv

Received: 17 March 2023; Revised: 01 May 2023;
Accepted: 05 June 2023; Available on-line:  20 June 2023

Biochemical indices of blood and tissue of the gastrocnemius muscle chronically alcoholized (for 3, 6 and 9 months) rats were studied. С60 fullerene aqueous solution (C60FAS) was administered orally as a pharmacological agent at a dose of 1 mg/kg daily throughout the experiment in a three routes: 1 h before alcohol intake (preventive regimen), together with alcohol (therapeutic regimen I) and 1 h after alcohol intake (therapeutic regimen II). Creatine phosphokinase (CPK), lactate dehydrogenase (LDH), catalase, superoxide dismutase, glutathione peroxidase (GPx) activity and the level of creatinine, lactate, hydrogen peroxide, reduced glutathione were estimated with clinical diagnostic kits. A pronounced upward trend in creatinine and lactate content, CPK and LDH activity with increasing degree of alcoholic myopathy during experiment was detected. Administration of C60FAS was shown to reduce the biochemical indices of muscle injury and to reduce oxidative processes by maintaining the balance between pro-oxidant and antioxidant systems. The maximum positive effect was observed when C60FAS was administered together with alcohol (therapeutic regimen I). The results indicate on C60 fullerene ability to correct the pathological condition of the muscular system arising from alcohol intoxication.

Comparison of blood biochemical indices in rats exposed to lead in macrodispersed form and nanoform

 I. A. Lazarenko, N. M. Melnikova

National University of Life and Environmental Sciences of Ukraine, Kyiv;
e-mail: ilazarenko2009@yandex.ru

It is shown that the increasing content of lead in blood (6.3 and 3.7 times) and liver (30.1 and 4.6 times) in rats after 14-days per os exposure both to lead acetate (macrodispersed form) and lead nanoparticles (nanoform) in a dose of 7 mg/100 g of body weight leads to the increased of activity of blood enzymes: alanine aminotransferase, aspartate aminotransferase, γ-glutamyltransferase, alkaline phosphatase, lactate dehydrogenase and a decrease of creatinine level. Lead in nanoform with slight accumulation, due to the greater elimination, expressed higher biological activity and reactivity as compared to macrodispersed form. Thus the exposure to lead in different dispersed form suggests metabolic disorders in rats, and accumulation of lead and biochemical changes are more expressed in the liver.