O. Motuziuk^1,2, D. Nozdrenko², S. Prylutska³,
K. Bogutska², O. Korotkyi², Yu. Prylutskyy²*

¹Lesya Ukrainka Volyn National University, Lutsk, Ukraine;
²Taras Shevchenko National University of Kyiv, Ukraine;
*e-mail: prylut@ukr.net;
³National University of Life and Environmental Science of Ukraine, Kyiv

Received: 17 March 2023; Revised: 01 May 2023;
Accepted: 05 June 2023; Available on-line:  20 June 2023

Biochemical indices of blood and tissue of the gastrocnemius muscle chronically alcoholized (for 3, 6 and 9 months) rats were studied. С₆₀ fullerene aqueous solution (C₆₀FAS) was administered orally as a pharmacological agent at a dose of 1 mg/kg daily throughout the experiment in a three routes: 1 h before alcohol intake (preventive regimen), together with alcohol (therapeutic regimen I) and 1 h after alcohol intake (therapeutic regimen II). Creatine phosphokinase (CPK), lactate dehydrogenase (LDH), catalase, superoxide dismutase, glutathione peroxidase (GPx) activity and the level of creatinine, lactate, hydrogen peroxide, reduced glutathione were estimated with clinical diagnostic kits. A pronounced upward trend in creatinine and lactate content, CPK and LDH activity with increasing degree of alcoholic myopathy during experiment was detected. Administration of C₆₀FAS was shown to reduce the biochemical indices of muscle injury and to reduce oxidative processes by maintaining the balance between pro-oxidant and antioxidant systems. The maximum positive effect was observed when C₆₀FAS was administered together with alcohol (therapeutic regimen I). The results indicate on C₆₀ fullerene ability to correct the pathological condition of the muscular system arising from alcohol intoxication.