Tag Archives: lipid profile

Extracellular matrix degradation products in the heart of rats with metabolic syndrome under chromium picolinate administration

O. Ye. Akimov1*, A. O. Mykytenko2, V. O. Kostenko1

1Department of Pathophysiology, Poltava State Medical University, Poltava, Ukraine;
*e-mail: o.akimov@pdmu.edu.ua;
2Department of Biological and Bioorganic Chemistry,
Poltava State Medical University, Poltava, Ukraine

Received: 22 May 2025; Revised: 24 July 2025;
Accepted: 12 September 2025; Available on-line: 17 September 2025

The populace of Ukraine shows a trend of increasing percentage of persons with obesity, complicated by metabolic syndrome (MetS), which causes damage to the heart extracellular matrix. According to recent studies chromium, picolinate (CrPIC) has the potential to attenuate lipid metabolism disorders and protect the extracellular matrix from degradation. The aim of this research was to estimate the blood lipid profile and the content of glycosaminoglycans, L-hydroxyproline and sialic acids in the heart of rats with simulated metabolic syndrome under Chromium picolinate administration. Mature male Wistar rats were divided into 4 groups of 6 animals each – control; metabolic syndrome induction; CrPIC administration; metabolic syndrome + CrPIC administration. Metabolic syndrome was reproduced by using a 20% fructose solution as the only source of water for 60 days. CrPIC was administered orally at a dose of 80 µg/kg daily for 60 days. The concentration of the heart extracellular matrix degradation proteins was determined spectrophotometrically in a 10% heart homogenate. CrPIC administration to healthy animals stimulated the accumulation of glycosaminoglycans chondroitin fraction in the rat heart. Metabolic syndrome modeling resulted in an increase in TG, TC and LDL-C blood levels, intensification of collagenolysis, degradation of glycoproteins and glycosaminoglycans with a predominance of the keratan-dermatan fraction. CrPIC administration to animals with metabolic syndrome reduced collagenolysis and glycoproteins degradation, changed the dominating fraction of glycosaminoglycans from keratan-dermatan to chondroitin in rat heart connective tissue indicating its potential to prevent cardiac tissue remodeling during metabolic syndrome.

Effect of electronic smoking (Vaping) on thyroid hormones level and lipid profile in men

N. Hasan1, N. A. Nasser2, A. D. Hussein3, O. A. Mohsein4,5*

1Ibn Sina University of Medical and Pharmaceutical Sciences, Baghdad, Iraq;
2College of Medicine, Al-Nahrain University, Baghdad, Iraq;
3Departement of Applied Chemistry, College of Applied Science,
University of Fallujah, Fallujah, Iraq;
4Department of Medical Laboratory Techniques,
Mazaya University College, Nasiriyah, Iraq;
5Central Laboratory Department, Thi-Qar Health Directorate,
*e-mail: osamaakram889@gmail.com

Received: 01 May 2024; Revised: 18 June 2024;
Accepted: 07 October 2024; Available on-line: 28 October 2024

In the last period, the market quickly became saturated with vaping devices available in many flavors and forms appealing to youth. Both traditional cigarette and e-cigarette smoking are known to potentially alter metabolic processes, including hormone production and to increase the risk of lung, heart and kidney diseases. The aim of the study was to estimate the level of thyroid hormones and lipids in the blood of young men who smoked traditional or e-cigarettes. A case-control study involved 200 men aged 24-25 years who smoked 5-7 h per day, divided into two groups (100 e-cigarette smokers and 100 cigarette smokers) and 50 healthy men who did not smoke. The levels of thyroid-stimulating hormone (TSH), free thyroxine (FT4), and free triiodothyronine (FT3) were measured using COBAS E411. The levels of total cholesterol, TG, LDL and HDL were estimated spectrophotometrically. No significant changes were found in thyroid hormone levels or lipid profiles, except for an increased TG content in the group of traditional cigarette smokers compared to the control group. Whereas in the blood of e-smokers, the increase in the level of FT3 and FT4 and a significant decrease in that of TSH, as well as the elevated content of total cholesterol, TG, and LDL, were detected compared to the control group. The results obtained indicate that e-smoking affects the function of the thyroid gland and lipid metabolism.

Personalised diet improve intestine microbiota and metabolism of obese rats

V. V. Bati1*, T. V. Meleshko1, O.V. Pallah1,
I. P. Zayachuk2, N. V. Boyko1

1RDE Centre of Molecular Microbiology and Mucosal Immunology, Uzhhorod National University, Ukraine;
2Department of Physiology and Pathophysiology, Uzhhorod National University, Ukraine;
*e-mail: victoria.bati@uzhnu.edu.ua

Received: 25 April 2020; Accepted: 07 July 2021

Recent research on human microbiome provide opportunities to develop functional foods of new generation that can regulate intestinal microbiota and the biochemical status of the individual. The aim of the study was to determine the effect of individually designed nutrition on the intestinal microbiota and metabolic parameters of rats. Outbred laboratory rats with obesity  were randomly divided into 9 groups (= 12) depending on the type of food ingredients taken orally for three months. The ratio of the intestinal commensal microorganisms main groups, as well as the lipid profile and the content of glucose, urea, calcium in the serum of animals were determined. It was shown that cholesterol level in the serum was reduced in experimental groups after consumption of lactobacilli suspension, blueberry juice, fermented milk drink based on lactobacilli, fermented milk drink with blueberry juice, sauerkraut. In most cases, the gut microbiome of experimental animals was characterized by a consistently high level of lacto and other beneficial bacteria and decreased amount of opportunistic microorganisms at the end of the experiment compared with animals in the control group. Based on the obtained data, we first proposed the principles of creating functional products by synergistically combining components of edible plants that act as prebiotics and microorganisms that act as probiotics for personalized use, targeted correction of intestinal microbiome and prevention of noncommunicable diseases.

Lipid profile parameters and oxidative processes intensity in the persons who have been affected by low doses of radiation

V. L. Sokolenko, S. V. Sokolenko

Bohdan Khmelnytsky National University of Cherkasy, Ukraine;
e-mail: sokolenko@ukr.net

Received: 10 October 2018; Accepted: 14 March 2019

The aim of the work was to analyze the relationship between the main parameters of lipid metabolism and the intensity of oxidative processes among the inhabitants of the territories contaminated by radionuclides as a result of the Chornobyl accident. We examined 50 persons from the control group and 50 persons from the territories of strengthened radioecological control (density of soil contamination by isotopes 137Cs 3.7∙104–18.5∙104 Bq/m2, 50 persons). All examined were the students aged 18 to 24, who had no acute illnesses during the study. We determined the parameters of lipid metabolism, oxidative processes and antioxidant system. A positive correlation of all analyzed lipid metabolism parameters (except for HDL-C) with MDA, ceruloplasmin and the index of oxidative stress was discovered. The highest values of correlation coefficients with oxidative stress indices were observed for low-density lipoprotein cholesterol. Under the conditions of additional emotional stress, the correlation coefficients between the main lipid metabolism and the intensity of oxidative processes increased. Persons who lived for a long time in areas contaminated with radionuclides form the risk group for the development and progression of inflammatory processes. The risk increases under the influence of additional factors of a stressful nature.