Tag Archives: obesity
Characterization of the blood coagulation system in morbidly obese patients
D. S. Korolova1, O. V. Hornytska1, A. S. Lavrik2,
N. M. Druzhyna1*, N. Prysyazhna3, T. M. Platonova1
1Palladin Institute of Biochemistry, National Academy of Science of Ukraine, Kyiv;
2Shalimov National Institute of Surgery and Transplantation,
National Academy of Medical Sciences of Ukraine, Kyiv;
3Shupyk National Healthcare University of Ukraine, Kyiv;
*e-mail: ndbiochem@gmail.com
Received: 18 April 2023; Revised: 05 May 2023;
Accepted: 7 September 2023; Available on-line: 12 September 2023
Obesity is a complex metabolic disorder that can be followed by blood coagulation disorders, atherosclerosis and atherothrombosis. In the present work, the levels of fibrinogen, soluble fibrin, D-dimer as well as protein C were measured in the blood plasma of 24 morbidly obese patients (the body mass index exceeds 40 kg/m2) to evaluate the risk of prothrombotic state. The study showed that near by 80% of patients had substantially increased fibrinogen concentration, 33% had increased concentration of soluble fibrin, 42% had increased level of D-dimer in blood plasma as compared to control. According to the results of individual analysis, the high level of fibrinogen and soluble fibrin while reduced protein C indicated the threat of thrombosis, which requires complex diagnostics to be identified. Therefore, simultaneous quantification of hemostatic system biomarkers in the blood plasma is the confident way to predict the risk of thrombotic complications in morbidly obese patients.
Correlation between adiponectin level and obesity as a risk factor for allergy disease
M. Spasovska1*, T. K. Panovska2
1General Hospital, Ohrid, R.Macedonia;
2Faculty of Pharmacy, Ss. Cyril and Methodius University, Skopje, R. Macedonia;
*e-mail: spasovskamilena@yahoo.com
Received: 28 November 2021; Revised: 30 June 2022;
Accepted: 29 September 2022; Available on-line: 06 October 2022
Much research has focused on the connection between two inflammatory conditions, allergic reactions and obesity which has led to a focus on adiponectin, hormone with anti-inflammatory properties secreted by adipose tissue. The aim of this study was to determine the association of adiponectin with obesity, as a risk factor for the development of allergic condition in order to rationalize approach to its treatment. Research methods for inflammatory markers and biochemical parameters involve immunoassay technique. Statistical analysis was performed with Student’s t-test, Wilcoxon T-test and coefficient of correlation. The study included apparently healthy subjects and patients with allergy conditions with confirmed presence of specific IgE, classified into 2 groups according to their body mass index (BMI). The obtained data showed negative correlation (cor = – 0.6), between adiponectin levels and BMI values. Thus, decreased level of adiponectin is associated with increased BMI. The mean values of adiponectin in the studied population, with high statistical differences between the groups (19.1 ± 1.5; 17.7 ± 0.9), (18.8± 1.1; 16.6 ± 1.0) demonstrated the relationship between low adiponectin level and development of obesity, and what, in turn, increasd risk of developing allergic conditions. The assumption was made that adiponectin may be used as a sensitive biochemical marker for early diagnostics of allergic reactions.
Growth hormone, growth hormone receptor and insulin-like growth factor serum levels in patients with obesity and food addiction
O. Avsar1*, S. Sancak2, I. Koroglu3, E. Avci4
1Hitit University, Department of Molecular Biology and Genetics, Corum, Turkey;
2Fatih Sultan Mehmet Education and Research Hospital, Department of Endocrinology, Istanbul, Turkey;
3Arapgir Ali Özge State Hospital, Department of Internal Medicine, Malatya, Turkey;
4Health Sciences University, Department of Biochemistry, Ankara, Turkey;
*e-mail: orcunavsar@hitit.edu.tr
Received: 27 May 2021; Accepted: 12 November 2021
Obesity is a public health problem that increasingly becomes widespread and causes various complications. Food addiction is a hedonic eating behavior characterized by overconsumption of palatable foods (i.e., foods involve a high amount of salt, sugar and fat). Disturbances in the growth hormone signaling pathway were shown to be associated with increased food intake and adiposity. The study aimed to determine the growth hormone (GH), growth hormone receptor (GHR), insulin, and insulin-like growth factor 1 (IGF-1) serum levels in individuals with obesity and food addiction. The present study involved 30 adults with obesity (23 females and 7 males) and 10 healthy adults (5 females and 5 males). 18 obese adults were diagnosed with food addiction, whereas only 2 individuals with food addiction were in the control group. GH, GHR, IGF-1 and insulin values were analyzed with ELISA kits. It was revealed that the obese subjects had significantly lower serum IGF-1 levels compared to healthy individuals (144.55±22.69 ng/ml vs 338.70±61.90 ng/ml, P < 0.001)). No significant differences in the GH, GHR and insulin levels between obese and control groups were detected (P > 0.05). No significant differences between the group with food addiction and the group without food addiction in terms of gender, age, weight, BMI, GH, GHR, insulin and IGF-1 levels were observed. Our study demonstrates that normal IGF-1 levels may be protective for the development of obesity. The serum levels of GH, GHR, insulin, IGF-1 are not associated with food addiction and, therefore, can not be used as novel markers of food addiction.
Personalised diet improve intestine microbiota and metabolism of obese rats
V. V. Bati1*, T. V. Meleshko1, O.V. Pallah1,
I. P. Zayachuk2, N. V. Boyko1
1RDE Centre of Molecular Microbiology and Mucosal Immunology, Uzhhorod National University, Ukraine;
2Department of Physiology and Pathophysiology, Uzhhorod National University, Ukraine;
*e-mail: victoria.bati@uzhnu.edu.ua
Received: 25 April 2020; Accepted: 07 July 2021
Recent research on human microbiome provide opportunities to develop functional foods of new generation that can regulate intestinal microbiota and the biochemical status of the individual. The aim of the study was to determine the effect of individually designed nutrition on the intestinal microbiota and metabolic parameters of rats. Outbred laboratory rats with obesity were randomly divided into 9 groups (n = 12) depending on the type of food ingredients taken orally for three months. The ratio of the intestinal commensal microorganisms main groups, as well as the lipid profile and the content of glucose, urea, calcium in the serum of animals were determined. It was shown that cholesterol level in the serum was reduced in experimental groups after consumption of lactobacilli suspension, blueberry juice, fermented milk drink based on lactobacilli, fermented milk drink with blueberry juice, sauerkraut. In most cases, the gut microbiome of experimental animals was characterized by a consistently high level of lacto and other beneficial bacteria and decreased amount of opportunistic microorganisms at the end of the experiment compared with animals in the control group. Based on the obtained data, we first proposed the principles of creating functional products by synergistically combining components of edible plants that act as prebiotics and microorganisms that act as probiotics for personalized use, targeted correction of intestinal microbiome and prevention of noncommunicable diseases.
Insulin resistance in obese adolescents and adult men modifies the expression of proliferation related genes
O. H. Minchenko1, Y. M. Viletska1, D. O. Minchenko1,2, V. V. Davydov3
1Palladin Institute of Biochemistry, National Academy of Sciences of Ukraine, Kyiv;
e-mail: ominchenko@yahoo.com;
2Bohomolets National Medical University, Kyiv, Ukraine
3SI “Institute of Children and Adolescent Health Care,
National Academy of Medical Sciences of Ukraine”, Kharkiv
Received: 11 December 2018; Accepted: 14 March 2019
Numerous data demonstrate that key regulatory factors, enzymes and receptors including HSPA5, MEST, SLC1A3, PDGFC, and ADM represent poly-functional, endoplasmic reticulum stress-dependent proteins, which control variable metabolic pathways. The expression level of genes of these proteins in the blood and subcutaneous adipose tissue of obese adolescents and adult men with and without insulin resistance was studied. It was shown that in blood of obese adolescents without insulin resistance the expression level of SLC1A3, HSPA5, MEST, and PDGFC genes was significantly increased, but development of insulin resistance led to down-regulation of these genes expression except HSPA5 gene as compared to the control group as well as to the group of obese adolescents without insulin resistance. At the same time, the expression level of ADM gene did not change significantly in obese adolescents without insulin resistance, but the development of insulin resistance led to down-regulation of this gene expression. In subcutaneous adipose tissue of obese adult men without insulin resistance the level of SLC1A3 gene expression was decreased, although ADM, MEST, and HSPA5 genes – increased. It was also shown that the development of insulin resistance in obese men affected the expression level of ADM and SLC1A3 genes only. Results of this investigation provide evidence that insulin resistance in obese adolescents and adult men is associated with specific changes in the expression of genes, which related to proliferation and development of obesity and insulin resistance as well as to endoplasmic reticulum stress and contribute to the development of obesity complications.
Diagnostic significance of biochemical indicators of liver fibrogenesis in adolescents with obesity
O. V. Buznytska
Kharkіv Medical Academy of Postgraduate Education,
V. N. Karazin Kharkіv National University, Ukraine
e-mail: ebuznickaa@ukr.net; missbuzelena@gmail.com
Received: 27 September 2018; Accepted: 13 December 2018
Non-alcoholic fatty liver disease occurs in most obese people, the main pathway of which is the process of fibrogenesis. The aim of this work was to determine the potential biomarkers for early diagnosis of liver fibrogenesis in adolescents with obesity. The levels of liver fibrosis markers, such as fibronectin, collagen type IV, N-terminal propeptides and C-terminal telopeptides of type I collagen, were assessed with the use of IFA method in serum of 226 patients with obesity aged 8-18 years. A significant increase in levels of type IV collagen and fibronectin was observed in children with obesity (P < 0.05). As diagnostic criteria for fibrogenesis and fibrolysis, the levels of N-terminal propeptides and C-terminal telopeptides of type I collagen, respectively, were determined. The serum level of N-terminal propeptides of type I collagen significantly exceeds the normal values in all children with obesity compared to the control group (P < 0.05). The biochemical markers (type IV collagen, fibronectin, N-terminal propeptides and C-terminal telopeptides of type I collagen) were proven to have high diagnostic informative value in the early diagnosis of liver fibrogenesis in obese adolescents. It was shown that the signs of fibrosis in non-alcoholic fatty liver disease already occur at the stage of steatosis.
The effect of N-stearoylethanolamine on adipocytes free cholesterol content and phospholipid composition in rats with obesity-induced insulin resistance
O. S. Dziuba, Ie. A. Hudz, H. V. Kosiakova, T. M. Horid’ko, V. M. Klimashevsky, N. M. Hula
Palladin Institute of Biochemistry, National Academy of Sciences of Ukraine, Kyiv;
e-mail: oksana.dziuba86@gmail.com
Obesity induces molecular changes that promote associated disorders, such as insulin resistance (IR) and type 2 diabetes. Low insulin sensitivity occurs primarily due to defects in the pathway of insulin action in target tissues, and there is a hypothesis that IR may originate in adipose tissue and is followed by dyslipidemia. In this study using methods of thin-layer and gas-liquid chromatography we investigated free cholesterol content and phospholipid composition of adipocytes of obesity-induced IR rats and its changes induced by the N-stearoylethanolamine (NSE) administration. The results we obtained demonstrated that free cholesterol content significantly increased in adipocytes of IR rats compared to control. The analysis of phospholipid composition indicated a reduction of phosphatidylcholine and the total content of phosphatidylinositol with phosphatidylserine, whereas the content of lysophosphatidylcholine, sphingomyelin and phosphatidylethanolamine increased in IR group compared to control. NSE administration caused a statistically significant decrease in total cholesterol level and had a considerable effect on normalization of individual phospholipids content. As far as NSE administration caused a statistically significant decrease in free cholesterol level and had a considerable effect on normalization of individual phospholipids content of adipocytes, we can consider NSE as a prospective compound worthy more complex investigation of its action under the pathological conditions.