D. M. Nozdrenko¹, O. O. Gonchar², N. E. Nurishchenko¹,
V. O. Stetska¹, T. Yu. Matviienko¹, Ya. V. Stepanyuk³,
K. I. Bogutska¹, Yu. I. Prylutskyy¹*

¹ESC “Institute of Biology and Medicine”, Taras Shevchenko National University of Kyiv, Ukraine;
²Bogomoletz Institute of Physiology, National Academy of Sciences of Ukraine, Kyiv;
³Medical Faculty, Lesya Ukrainka Volyn National University, Lutsk, Ukraine;
*e-mail: prylut@ukr.net

Received: 05 September 2024; Revised: 14 October 2024;
Accepted: 21 November 2024; Available on-line: 17 December 2024

Open injuries are one of the most common skeletal muscle traumas. The study aimed to estimate the effect of the oral administration of C₆₀ fullerene aqueous solution (C₆₀FAS) daily at a dose of 1 mg/kg on the restoration of rat skeletal muscle functional activity on the 5^th, 10^th and 15^th day after the open trauma. Male Wistar rats were randomly divided into three groups of 12 animals in each: control, with muscle injury and with muscle injury+C₆₀FAS. The isolated gastrocnemius muscle was subjected to open injury by transverse dissection with a depth of 1 mm. Stimulation of muscle efferents was carried out by electrical impulses gene­rated using a strain gauge generator. The content of C-reactive protein, creatinine, lactate, reduced glutathione and the activity of catalase and superoxide dismutase in the rat blood were determined. According to the data obtained, application of C₆₀FAS promotes the restoration of the functional activity of injured muscle, which was confirmed by a significant increase in gastrocnemius muscle force impulse, attenuation of the inflammatory and development of fatigue and normalization of pro- and antioxidant balance in the process of regeneration.