Tag Archives: paraoxonase
Metabolic effects of broccoli sprouts in mice with cafeteria diet-induced obesity
M. V. Ivanochko, T. R. Dmytriv, I. M. Yatskiv,
M. M. Bayliak, V. I. Lushchak*
Department of Biochemistry and Biotechnology,
Vasyl Stefanyk Carpathian National University, Ivano-Frankivsk, Ukraine;
*e-mail: volodymyr.lushchak@cnu.edu.ua
Received: 05 July 2025; Revised: 29 September 2025;
Accepted: 28 November 2025; Available on-line: 23 December 2025
Broccoli sprouts (BS) are rich in bioactive compounds with reported antioxidant and anti-inflammatory properties. In this study, a cafeteria diet (CD) was used as a model to study diet-induced obesity in animals. The aim of the study was to evaluate the effects of dietary BS supplementation on metabolic parameters in middle-aged male mice subjected to a cafeteria diet (CD) containing such additional components (w/w) as sweet peanuts (28%), milk chocolate (28%) and chocolate cracker (11%). Mice were fed on CD over 20 weeks, after that, blood was collected, mice were sacrificed, liver and adipose tissue were collected and weighed. The levels of glucose, triacylglycerides (TAG), and cholesterol were determined with a diagnostic kit (Reagent, Dnipro, Ukraine), that of IL-1β – by ELISA. Paraoxonase (PON) activity in blood was determined by monitoring p-nitrophenol formation. Mice fed on the CD alone exhibited higher caloric intake without significant body mass gain, but demonstrated elevated liver mass, hyperglycemia, hypertriglyceridemia, and decreased PON activity relative to those fed on the standard diet. Inclusion of BS (2.5, 5 or 10% w/w) in the CD prevented the rise in TAG level and preserved PON activity. However, BS in higher doses (5 and 10%) increased visceral fat accumulation and further elevated blood glucose levels. In contrast, BS supplementation in a standard diet reduced circulating TAG and inflammatory markers without affecting adipose tissue distribution. These findings indicate a dual role of BS in metabolic regulation: while beneficial in reducing oxidative and inflammatory markers, BS may aggravate visceral adiposity and glycemic imbalance in an obesogenic context.
The relationship between serum ferritin levels and serum lipids and HDL function with respect to age and gender
Hamit Yasar Ellidag1*, Esin Eren1, Mehmet Akdag2,
Ozlem Giray1, Kemal Kiraz3, Necat Yilmaz1
1Central Laboratories of Antalya Training and Research Hospital,
University of Medical Sciences, Ministry of Health, Antalya, Turkey;
2ENT Clinic of Antalya Ataturk State Hospital, Ministry of Health, Antalya, Turkey;
3Department of Pulmonology, Antalya Ataturk State Hospital,
Ministry of Health, Antalya, Turkey;
e-mail: hayael1980@hotmail.com
Elevated serum ferritin (SFer) levels have been associated with chronic diseases such as coronary heart disease and diabetes mellitus type 2. The aim of this study was to examine the relationship between SFer levels and serum lipid parameters, and how this relation changes in terms of age and gender. Additionally, we investigated a possible relationship between SFer levels and high-density lipoprotein (HDL) function. SFer levels and lipid panel (total cholesterol (TC), triglyceride (TG), low-density lipoprotein-cholesterol (LDL-C) and HDL-C) of 4205 people (3139 women, 1066 men) were examined retrospectively. Study population was classified according to age and gender. Separately, 100 subjects (52 women, 48 men) were randomly recruited to investigate the relation between SFer levels, and HDL dependent paraoxonase-1 (PON1) and arylesterase (ARE) activities. In all age groups, women’s SFer levels were found to be significantly lower and HDL-C levels significantly higher compared to men. In the 50-70 ages range, TC and LDL-C levels of women were found to be significantly higher than those of men (P < 0.01). SFer levels tended to increase with age in women. Correlation analyses revealed a negative correlation between levels of SFer and HDL-C, while positive correlations existed between levels of SFer, and TC, TG and LDL-C. There was no significant correlation between SFer levels and PON1 or ARE activities. The finding that increased SFer levels are accompanied by increased serum TC, TG and LDL-C levels may help us to explain the increased risk of metabolic disorders and cardiovascular disease in postmenopausal women.