Tag Archives: proteins

Scientific investigations of the Nobel prize winner Emil Fischer as a launching pad for the development of biochemistry: a brief overview

T. V. Danylova1, S. V. Komisarenko2

1National University of Life and Environmental Sciences of Ukraine, Kyiv;
e-mail: danilova_tv@ukr.net;
2Palladin Institute of Biochemistry, National Academy of Sciences of Ukraine, Kyiv;
e-mail: svk@biochem.kiev.ua

Modern biochemistry and molecular biology would be impossible without discoveries in related fields of science. This paper aims to outline briefly the main stages of scientific activity of a Nobel Prize winner 1902 – German chemist Hermann Emil Fischer, one of the leading chemists of all times. Emil Fischer was a brilliant multifaceted scientist who left his mark in organic chemistry, physiology, medicine, gave impetus to the development of biochemistry. His insights into the structures of sugars, enzymes, proteins, and purines have become a launching pad for the further development of biochemistry and molecular biology. His contribution to the natural sciences was immense; some chemical reactions and concepts were named after him. This prominent scientist was honored with a number of awards.

Modification of spectrophotometric method of determination of protein carbonyl groups

O. V. Zaytseva, S. G. Shandrenko

Palladin Institute of Biochemistry, National Academy of Sciences of Ukraine, Kyiv;
e-mail: Olgga__89@mail.ru

Basing on detailed study of optical absorption spectra of protein-hydrazones a modification of the spectrophotometric method determination of the protein carbonyl groups has been proposed. For the hydrazone adduct determination we proposed to measure the optical absorption at three wavelengths (320, 370 and 420 nm) instead of only one (at 370 nm). Two additional wavelengths are used for the linear approximation of the background optical spectrum. This modification improves the specificity and reduces the error when determining the content of CO groups in proteins.

Legume-rhizobium symbiosis proteomics: achievements and perspectives

Iu. Iu. Kondratiuk, P. M. Mamenko, S. Ya. Kots

Institute of Plant Physiology and Genetics, National Academy of Sciences of Ukraine, Kyiv;
e-mail: kondratyuk_yulya@ukr.net

The present review contains results of proteo­mic­ researches of legume-rhizobium symbiosis. The technical difficulties associated with the methods of obtaining protein extracts from symbiotic structures and ways of overcoming them were discussed. The changes of protein synthesis under formation and functioning of symbiotic structures were shown.  Special attention has been given to the importance of proteomic studies of plant-microbe structures in the formation of adaptation strategies under adverse environmental conditions. The technical and conceptual perspectives of legume-rhizobium symbiosis proteomics were shown.