Tag Archives: saturated and unsaturated fatty acids
Fatty acids composition of inner mitochondrial membrane of rat cardiomyocytes and hepatocytes during hypoxia-hypercapnia
S. V. Khyzhnyak, S. V. Midyk, S. V. Sysoliatin, V. М. Voitsitsky
National University of Life and Environmental Sciences of Ukraine, Kyiv;
е-mail: khs2014@ukr.net
We studied the influence of hypoxic-hypercapnic environment under the effect of hypothermia (artificial hibernation) on fatty acids spectrum of inner mitochondrial membrane (IMM) lipids of rat cardiomyocytes and hepatocytes. Specific for cellular organelles redistribution of IMM fatty acids was determined. It led to the reduction of total amount of saturated fatty acids (SFAs) and increase of unsaturated fatty acids (UFAs) in cardiomyocytes and to the increase of SFAs and decrease of UFAs in hepatocytes. The decrease in the content of oleic acid and increased content of arachidonic and docosahexaenoic acids in IMM were shown. This may be due to their role in the regulatory systems during hibernation, as well as following exit therefrom. It is assumed that artificial hibernation state is characterized by the stress reaction leading to optimal readjustment of fatty acids composition of membrane lipids, which supports functional activity of mitochondria in hepatocytes and cardiomyocytes.
Influence of antihypertensive and metabolic drugs on fatty acids content of lipids in cardiomyocytes of rats with spontaneous hypertension
A. M. Puzyrenko1, I. S. Chekman1, T. S. Bruzgina2, N. O. Gorchakova1
1O.O. Bohomolets National Medical University, Kyiv, Ukraine;
2Institute for Problems of Pathology of O. O. Bohomolets National Medical University, Kyiv, Ukraine;
e-mail: a.puzyrenko@ukr.net
Changes in fatty acid composition of lipids are an important factor in the development of arterial hypertension. Therefore, this is very important to research the role of fatty acid spectrum of the blood and tissues in the development of hypertension. The search for effective metabolic drugs and antihypertensive drugs which would have the additional ability to influence the fatty acid composition of lipids in cells is also important today. We have found that hypertensive rats demonstrate the essential decrease of the amount of saturated fatty acids (sFA) and high content of unsaturated fatty acids (usFA). Application of amlodipine increases the level of sFA compared with animals without treatment and the level of usFA tends to decrease. A similar pattern is observed when using bisoprolol and combination of amlodipine with bisoprolol, although the combination is characterized by more significant changes in FA composition of lipids in cardiomyocytes. Treatment with metabolic drug elgacin leads to full recovery of saturated and unsaturated fatty acids in the cardiomyocytes. During the treatment with combinations of amlodipine with elgacin and bisoprolol with elgacin the level of both types of AF was not significantly different from the elgacin action in monotherapy. This study demonstrates the modification of the FA composition of lipids in cardiomyocytes of the spontaneously hypertensive rats. The investigated drugs exhibit a normalizing influence on the ratio between sFA and usFA in cardiomyocytes of the hypertensive rats.