Ukr.Biochem.J. 2013; Volume 85, Issue 4, Jul-Aug, pp. 67-74


Influence of antihypertensive and metabolic drugs on fatty acids content of lipids in cardiomyocytes of rats with spontaneous hypertension

A. M. Puzyrenko1, I. S. Chekman1, T. S. Bruzgina2, N. O. Gorchakova1

1O.O. Bohomolets National Medical University, Kyiv, Ukraine;
2Institute for Problems of Pathology of O. O. Bohomolets National Medical University, Kyiv, Ukraine;

Changes in fatty acid composition of lipids are an important factor in the development of arterial hypertension. Therefore, this is very important to research the role of fatty acid spectrum of the blood and tissues in the development of hypertension. The search for effective metabolic drugs and antihypertensive drugs which would have the additional ability to influence the fatty acid composition of lipids in cells is also important today. We have found that hypertensive rats demonstrate the essential decrease of the amount of saturated fatty acids (sFA) and high content of unsaturated fatty acids (usFA). Application of amlodipine increases the level of sFA compared with animals without treatment and the level of usFA tends to decrease. A similar pattern is observed when using bisoprolol and combination of amlodipine with bisoprolol, although the combination is characterized by more significant changes in FA composition of lipids in cardiomyocytes. Treatment with metabolic drug elgacin leads to full recovery of saturated and unsaturated fatty acids in the cardiomyocytes. During the treatment with combinations of amlodipine with elgacin and bisoprolol with elgacin the level of both types of AF was not significantly different from the elgacin action in monotherapy. This study demonstrates the modification of the FA composition of lipids in cardiomyocytes of the spontaneously hypertensive rats. The investigated drugs exhibit a normalizing influence on the ratio between sFA and usFA in cardiomyocytes of the hypertensive rats.

Keywords: , , , , , ,


  1. Rousseau D, Héliès-Toussaint C, Raederstorff D, Moreau D, Grynberg A. Dietary n-3 polyunsaturated fatty acids affect the development of renovascular hypertension in rats. Mol Cell Biochem. 2001 Sep;225(1-2):109-19. PubMed
  2. Zicha J, Kunes J, Devynck MA. Abnormalities of membrane function and lipid metabolism in hypertension: a review. Am J Hypertens. 1999 Mar;12(3):315-31. Review. PubMed, CrossRef
  3. Sarafidis PA, Bakris GL. Non-esterified fatty acids and blood pressure elevation: a mechanism for hypertension in subjects with obesity/insulin resistance? J Hum Hypertens. 2007 Jan;21(1):12-9. Review. PubMed, CrossRef
  4. Lizogub VG,  Artemchuk OО, Briuzgina TS,  Voloshina OО. Changes in fatty-acid composition of triglycerides of blood plasma in patients with unstable angina and their dynamics under statin influence. Heart Vessels. 2009;(3(27)):71-76.
  5. Titov VN. Very low density and low density lipoproteins: function, transport of fatty acids and diagnostic role (lecture). Klin Lab Diagn. 2000 Nov;(11):25-32. Russian. PubMed
  6. Puzyrenko A., Gorchakova N., Chekman I., Dovgan’ R. Calcium channel blockers: current aspects of application in medical practice.  Ukr Sci Med Youth J. 2012;(3):30-34.
  7. Puzyrenko A., Zagorodnyy M., Gorchakova N. β-Adrenoblockers: modern clinical and pharmacological aspects of application in medical practice. Ukr Sci Med Youth J. 2012;(1):9-12.
  8. Yakovleva LV, Ivakhnenko OK, Sakharova TS.  Clin Pharmacy. 2004;8(3):41-44.
  9. Yakovleva LV, Sakharova TS. Rep Vinnytsia Nat Med Univ. 2007;(11(2)):801-802.
  10. Markel AL, Maslova LN, Shishkina GT, et al. Pathophysiological analysis of risk factors of hypertension and atherosclerosis. Novosibirsk,1992.  72p.
  11. Folch J, Lees M, Sloane Stanley GH.  A simple method for the isolation and purification of total lipides from animal tissues. J Biol Chem. 1957 May;226(1):497-509. PubMed
  12. Siniak KM, Orgel’ MIa, Kruk VI. Method of preparation of blood lipids for gas-chromatographic analysis. Lab Delo. 1976;(1):37-41. Russian. PubMed
  13.  Puzyrenko A, Gorchakova N, Antonenko L, Chekman I. Study of membranoprotektive antihypertensive drug activity in rats with spontaneous hypertension and features of their interaction with metabolitotropic preparations. Ukr Sci Med Youth J. 2011;(2):30-33.
  14. Puzyrenko AM, Chekman IS, Kuftyreva TP, Gorchakova NO. The effect of amlodipine on the myocardial ultrastructure of the hypertensive rats. J Int  Sci Publ. 2012;6:351-358.
  15. Puzyrenko AM, Chekman IS, Gorchakova NO, Belenichev IF. Curr Iss Pharm Med Sci Pract. 2012;(2):31.
  16. Novgorodtseva TP, Kantur TA, Karaman YK, Antonyuk MV, Zhukova NV. Modification of fatty acids composition in erythrocytes lipids in arterial hypertension associated with dyslipidemia. Lipids Health Dis. 2011 Jan 21;10:18.  PubMed, PubMedCentral, CrossRef
  17. Daly MM. Effects of hypertension on the lipid composition of rat aortic intima-media. Circ Res. 1972 Sep;31(3):410-6. PubMedCrossRef
  18. Oliveira TR, Lamy MT, De Paula UM, Guimarães LL, Toledo MS, Takahashi HK, Straus AH, Lindsey CJ, Paiva TB. Structural properties of lipid reconstructs and lipid composition of normotensive and hypertensive rat vascular smooth muscle cell membranes. Braz J Med Biol Res. 2009 Sep;42(9):844-53. PubMed, CrossRef
  19.  Mason RP, Walter MF, Trumbore MW, Olmstead EG Jr, Mason PE. Membrane antioxidant effects of the charged dihydropyridine calcium antagonist amlodipine. J Mol Cell Cardiol. 1999 Jan;31(1):275-81. PubMed, CrossRef
  20. Devipriya N, Sudheer AR, Vishwanathan P, Menon VP. Modulatory potential of ellagic acid, a natural plant polyphenol on altered lipid profile and lipid peroxidation status during alcohol-induced toxicity: a pathohistological study. J Biochem Mol Toxicol. 2008 Mar-Apr;22(2):101-12. PubMed, CrossRef

Creative CommonsThis work is licensed under a Creative Commons Attribution 4.0 International License.