Tag Archives: selenium

Effect of selenium and nano-selenium on cisplatin-induced nephrotoxicity in albino rats

M. M. A. Shafaee1, H. S. Mohamed2, S. A. Ahmed1, M. A. Kandeil3

1Chemistry department, Faculty of Science, Beni-Suef University, Egypt;
2Research Institute of Medicinal and Aromatic Plants, Beni-Suef University, Egypt;
3Biochemistry department, Faculty of Veterinary medicine, Beni-Suef University, Egypt;
e-mail: husseinshaban@science.bsu.edu.eg

Received: 05 July 2019; Accepted: 18 October 2019

Cisplatin is commonly used as a chemotherapeutic agent useful in the treatment of several forms of cancer, but its use is limited due to the undesirable side effects of nephrotoxicity. Most of the previous researches found a positive effect of using selenium as an antioxidant on the toxicity of cisplatin during short term administrations  although the recommended dose regimen of cisplatin in chemotherapy is multiple successive administration every three or four weeks depending on the type of the tumor. The aim of this study was to examine the effects of long term usage of selenium or nano-selenium on cisplatin-induced nephrotoxicity in albino rats. Forty rats were divided into equal four groups, 1st group as a control injected with normal saline, 2nd group injected with cisplatin 6 mg/kg every 21 days for 70 days (experimental period), 3rd group injected with cisplatin 6 mg/kg plus intramuscular injection 0.1 mg/kg selenium in the form of sodium selenite every 3 days during the experimental period, the 4th group injected with cisplatin 6 mg/kg plus intramuscular injection 0.1 mg/kg nano-selenium every 3 days during the experimental period. The results indicated that selenium or nano-selenium exerted an antioxidant effect through increasing the level of antioxidant enzymes in both serum and kidney tissue, while, it shows a negative effect on kidney function through increasing serum urea and creatinine concentrations and causing abnormal morphology of kidney tissue for rats treated with cisplatin during experimental period.

The effect of selenium haemoglobin an resistant for photooxidative processes

 T. M. Huseynov, F. R. Yahyayeva, R. T. Guliyeva

Institute of Physics, National Academy of Sciences of Azerbaijan;
e-mail: thuseynov@physics.ab.az

On an example of a guinea pig it is shown that exogenous selenium (0.5 mg Na2SeO3 per 1 kg of the animal weight) during 2-hour exposition in the animal organism increases the resistance to the photo-induced oxidation of haemoglobin in erythrocyte lysates without additional stimulation of glutathione peroxidase mechanism of haemoglobin protection by exogenous selenium. It is shown that the saturation of haemoglobin fractions by selenium hampers the oxidative modification of haemoglobin.
Using pregnancy of women as a natural model­ of selenium-deficiency condition, it has been shown that physiological debilitation of saturation erythrocytes with selenium, including haemoglobin fractions of lysates erythrocytes caused debilitation of resistance of haemoglobin to photo-oxidative destruction. Under these conditions not only activity of enzyme glutathione peroxidise in erythrocyte lysates, but also the peroxidase activi­ty of haemoglobin (in the presence of glutathione) were decreased. It is more characteristic of erythrocyte lysates with a less content of selenium, i.e. for the erythrocytes of women on late terms of pregnancy that testifies to the presence of certain relation between haemoglobin saturation with selenium and its peroxidase activity (in the presence of glutathione).

Relationship between RNA/DNA ratio, growth rate and accumulation of selenium in the cells of wheat leaves under the influence of minerals analcime and trepel

O. I. Martynenko1, T. K. Kyrylenko1, N. V. Zaimenko2, M. M. Antonyuk3,
A. V. Stepanyugin1, D. P. Plodnik4, D. M. Hovorun1,4

1Institute of Molecular Biology and Genetics, National Academy of Sciences of Ukraine, Kyiv;
2M. M. Gryshko National Botanical Gardens, National Academy of Sciences of Ukraine, Kyiv;
3National University of Food Technonogies, Kyiv, Ukraine;
4Institute of High Technologies, Taras Shevchenko National University of Kyiv, Ukraine;
e-mail: Martynenko.Olena@gmail.com

We studied specific effects of different doses of natural minerals – analcime (An) and trepel (Tr) – on the growth rate, selenium (Se) content and functional activity of the genome of wheat leaves measured by the RNA/DNA ratio. Our results show that under the influence of An and Tr, especially at low doses (25 mg/100 g sand), there is a significant increase in the content of Se, increased growth rate of leaves of wheat seedlings and decreased RNA/DNA ratio. We have found significant correlations between studied parameters. Our fin­dings suggest that the RNA/DNA ratio can be used as a convenient, reliable indicator of the biological activity of minerals An and Tr, and for quantitative express-estimation of their impact on plant organisms.