Ukr.Biochem.J. 2014; Volume 86, Issue 5, Sep-Oct, pp. 89-94


Relationship between RNA/DNA ratio, growth rate and accumulation of selenium in the cells of wheat leaves under the influence of minerals analcime and trepel

O. I. Martynenko1, T. K. Kyrylenko1, N. V. Zaimenko2, M. M. Antonyuk3,
A. V. Stepanyugin1, D. P. Plodnik4, D. M. Hovorun1,4

1Institute of Molecular Biology and Genetics, National Academy of Sciences of Ukraine, Kyiv;
2M. M. Gryshko National Botanical Gardens, National Academy of Sciences of Ukraine, Kyiv;
3National University of Food Technonogies, Kyiv, Ukraine;
4Institute of High Technologies, Taras Shevchenko National University of Kyiv, Ukraine;

We studied specific effects of different doses of natural minerals – analcime (An) and trepel (Tr) – on the growth rate, selenium (Se) content and functional activity of the genome of wheat leaves measured by the RNA/DNA ratio. Our results show that under the influence of An and Tr, especially at low doses (25 mg/100 g sand), there is a significant increase in the content of Se, increased growth rate of leaves of wheat seedlings and decreased RNA/DNA ratio. We have found significant correlations between studied parameters. Our fin­dings suggest that the RNA/DNA ratio can be used as a convenient, reliable indicator of the biological activity of minerals An and Tr, and for quantitative express-estimation of their impact on plant organisms.

Keywords: , , ,


  1. Zaimenko N. V. Scientific principles of structural and functional design of synthetical biocenoses in the “soil-plant-soil” system. K.: Nauk. Dumka, 2008. 303 p. (In Ukrainian).
  2. Zelentsov V., Datsko T. Thermodynamics of fluorine adsorption onto modified trepel. Termotehnica. 2013;(1):25-30.
  3. Kudrenko I. K,. Levon V. F., Zaimenko N. V., Moroz P. A. Using of analcime for reducing the content of phenolic compounds in soil under fruit plants. Plant Introduction. 2011;(3):93-97. (In Ukrainian).
  4. Rositska N. V. Increasing of lawn stability to different water conditions. Plant Introduction. 2011;(2):104-108. (In Ukrainian).
  5. Ralf MM, Epstein E, Falk RH. Silicon deprivation causes physical abnormalities in wheat (Triticum aestivum L.). J. Plant Physiol. 1997 Jan;151(4):497-501. CrossRef
  6. Currie HA, Perry CC. Silica in plants: biological, biochemical and chemical studies. Ann Bot. 2007 Dec;100(7):1383-9. Epub 2007 Oct 6. PubMed, PubMedCentral, CrossRef
  7. Nadarinskaya M. A., Kozinets T. G., Kvetkovs­kaya A. V., Golushko O. G. Influence of trepel on productivity and mineral content of the blood for cows in the first third of the period of lactation. Book of scientific works of VNAU. Feeding the animals and feed technology. 2011;50(10):10-15. (In Russian).
  8. Rockman MV, Kruglyak L. Genetics of global gene expression. Nat Rev Genet. 2006 Nov;7(11):862-72. Review. PubMed, CrossRef
  9. Heinrich S, Valentin K, Frickenhaus S, John U, Wiencke C. Transcriptomic analysis of acclimation to temperature and light stress in Saccharina latissima (Phaeophyceae). PLoS One. 2012;7(8):e44342. Epub 2012 Aug 28.  PubMed, PubMedCentralCrossRef
  10. Martynenko O. I., Kyrylenko T. K., Stepanyugin A. V., Plodnik D. P., Hovorun D. M. How do exogenous chemical factors impact on the activity of the wheat genome? A study using RNA. DNA ratio. Rep. National Acad. Sci. Ukraine. 2014;(1):130-136. (In Ukrainian).
  11. Martynenko OI, Kyrylenko TK, Stepa­nyugin AV, Plodnik DP, Hovorun DM. Quantitative estimation of genetically determined response in wheat leaves to the impact of chemical factors. Ukr Bioorgan Acta. 2013;(2):21-24. (In Ukrainian).
  12. Ratnikov AN, Sanzharov NI, Sviri­denko DG, Zhigareva TL. Effectiveness of complex fertilizers of new generation on radioactive contaminated lands. Geophys. Res. Abstr. 2012;14:14110.
  13. Zhu YG, Pilon-Smits EA, Zhao FJ, Williams PN, Meharg AA. Selenium in higher plants: understanding mechanisms for biofortification and phytoremediation. Trends Plant Sci. 2009 Aug;14(8):436-42. Epub 2009 Aug 6. Review. PubMed, CrossRef
  14. Pazurkiewicz-Kocot K., Kita A., Pietruszka M. Effect of selenium on Magnesium, iron, manganese, copper and zinc accumulation in corn treated by indole-3-acetic acid. Commun. Soil Sci. Plant Anal. 2008;39(15-16):2303–2318. CrossRef
  15. Levine HG, Sharek KM, Johnson KM, Stryjewski EC, Prima VI, Martynenko OI, Piastuch WC. Growth protocols for etiolated soybeans germinated within BRIC-60 canisters under spaceflight condition.  Adv Space Res. 2000;26(2):311-314. PubMed, CrossRef
  16. Ermakov VV. Fluorometric determination of selenium in animal products, organs (tissues) of animals and objects of the environment. Methodological guidelines for the definition of pesticides in biological objects. M.: VASHNIL. 1985:28-35. (In Russian).
  17. Spirin AS. Spectrophotometric estimation of total content of nucleic acids. Biokhimiia. 1958;23(5):656-62. (In Russian). PubMed
  18. Martynenko EI, Kyrylenko TK, Alkhi­mova EG. The method for isolation of DNA from plant. Rep. National Acad. Sci. Ukraine. 2004;(7):171-175. (In Ukrainian).
  19. Tkachuk EK, Kuz’menko LM, Nyzhko VF. Regulation of mineral nutrition and plant productivity. K.: Nauk. dumka, 1991. 172 p. (In Russian).
  20. Reef R, Ball MC, Feller IC, Lovelock CE. Relationships among RNA: DNA ratio, growth and elemental stoichiometry in mangrove trees. Func. Ecology. 2010;24(5):1064-1072. CrossRef

Creative CommonsThis work is licensed under a Creative Commons Attribution 4.0 International License.