Tag Archives: sialic acids

Extracellular matrix degradation products in the heart of rats with metabolic syndrome under chromium picolinate administration

10.09.2025   Uncategorized   No comments

O. Ye. Akimov1*, A. O. Mykytenko2, V. O. Kostenko1

1Department of Pathophysiology, Poltava State Medical University, Poltava, Ukraine;
*e-mail: o.akimov@pdmu.edu.ua;
2Department of Biological and Bioorganic Chemistry,
Poltava State Medical University, Poltava, Ukraine

Received: 22 May 2025; Revised: 24 July 2025;
Accepted: 12 September 2025; Available on-line: 17 September 2025

The populace of Ukraine shows a trend of increasing percentage of persons with obesity, complicated by metabolic syndrome (MetS), which causes damage to the heart extracellular matrix. According to recent studies chromium, picolinate (CrPIC) has the potential to attenuate lipid metabolism disorders and protect the extracellular matrix from degradation. The aim of this research was to estimate the blood lipid profile and the content of glycosaminoglycans, L-hydroxyproline and sialic acids in the heart of rats with simulated metabolic syndrome under Chromium picolinate administration. Mature male Wistar rats were divided into 4 groups of 6 animals each – control; metabolic syndrome induction; CrPIC administration; metabolic syndrome + CrPIC administration. Metabolic syndrome was reproduced by using a 20% fructose solution as the only source of water for 60 days. CrPIC was administered orally at a dose of 80 µg/kg daily for 60 days. The concentration of the heart extracellular matrix degradation proteins was determined spectrophotometrically in a 10% heart homogenate. CrPIC administration to healthy animals stimulated the accumulation of glycosaminoglycans chondroitin fraction in the rat heart. Metabolic syndrome modeling resulted in an increase in TG, TC and LDL-C blood levels, intensification of collagenolysis, degradation of glycoproteins and glycosaminoglycans with a predominance of the keratan-dermatan fraction. CrPIC administration to animals with metabolic syndrome reduced collagenolysis and glycoproteins degradation, changed the dominating fraction of glycosaminoglycans from keratan-dermatan to chondroitin in rat heart connective tissue indicating its potential to prevent cardiac tissue remodeling during metabolic syndrome.

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Carbohydrate composition of rat intestine surface mucus layer after ceftriaxone treatment

13.12.2016   Uncategorized   No comments

Yu. V. Holota, Ya. A. Olefir, T. V. Dovbynchuk, G. M. Tolstanova

Educational and Scientific Centre Institute of Biology and Medicine,
Taras Shevchenko National University of Kyiv, Ukraine;
e-mail: gtolstanova@gmail.com

The epidemiological studies have shown that antibiotic treatment increases the susceptibility to inflammatory bowel disease development. The disturbance of mucus layer integrity might be one of the possible mechanisms. The aim of the present study was to investigate the effect of antibiotic ceftriaxone treatment on glycoproteins level and its carbohydrate composition in surface mucus layer of rat intestine. The study was done on male Wistar rats (140-160 g). Ceftriaxone (300 mg/kg, i.m.) was administered once a day for 14 days. The surface mucus from terminal ileum and colon were collected on the 15th, 29th and 72nd days of the experiment. Total level of mucus glycoproteins, hexoses, hexosamines, fucose and sialic acids were measured. Ceftriaxone administration did not affect the levels of glycoproteins in rat ileum. In the colon, the levels of glycoprotein were 1.3-fold decreased (Р < 0.05) on the 72nd day of the experiment. These changes were accompanied by the 1.2-fold decrease of hexoses (Р < 0.05) and 3.1-fold (Р < 0.05) decrease of fucose level and 1.5-fold (Р < 0.05) increase of the levels of sialic acids in the surface mucus of the rat colon. Thus, ceftriaxone administration induces the long-term changes in the levels of glycoproteins and carbohydrates composition in the rat colon surface mucus. This could potentially explain the susceptibility to inflammatory bowel disea­ses development.

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