Tag Archives: zinc
Metallothioneins involment in the pathogenesis of synovial tissue inflammation in rats with acute gonarthritis
T. R. Matskiv1,2, D. V. Lytkin3, S. K. Shebeko3, V. V. Khoma2,
V. V. Martyniuk2, L. L. Gnatyshyna1,2, O. B. Stoliar2*
1I. Horbachevsky Ternopil National Medical University, Department of General Chemistry, Ternopil, Ukraine;
2Ternopil Volodymyr Hnatiuk National Pedagogical University, Department of Chemistry and Methods its Teaching, Ternopil, Ukraine;
3National University of Pharmacy, Educational and Scientific Institute of Applied Pharmacy, Kharkiv, Ukraine;
*e-mail: Oksana.Stolyar@tnpu.edu.ua
Received: 02 June 2021; Accepted: 22 September 2021
Zinc (Zn) is involving in the suppressing of inflammation. However, its functionality in the knee joint under the gonarthritis (GA) is not elucidated. The aim of this study was to investigate the participation of Zn-buffering and stress responsive proteins metallothioneins (MTs) in the pathogenesis of the synovial tissues under the experimental acute GA. The inflammation was induced in rats by intra-articular administration of carrageenan. The concentrations of MTs total protein (MTSH), Zn-bound protein (Zn-MTs), total Zn concentration in the tissue, the indexes of oxidative stress and cholinesterase activity were determined. The level of sialic acids was indicated in the blood serum. The enhancing of sialic acids concentration by 42% and cholinesterase depletion confirmed the pathology. In the animals with GA, total level of Zn in the tissue was correspondent to control. However, the MTSH and Zn-MT levels were elevated (by 79 and 46% respectively). This disproportionate rate can be due to partial oxidation of thiols. The superoxide dismutase activity was elevated, radical scavenging activity and protein carbonylation were correspondent to control, but the levels of catalase, glutathione–S-transferase and glutathione were decreased by 28-44%, and lipid peroxidation (TBARS) was increased by 59% compared to control group. Principal Component Analysis confirmed the strong interrelations between MTs and peroxide-related oxidative stress indexes. This preliminary study provides the basis for the understanding of the reason for Zn imbalance in the acute GA as the result of the impairment of thiol redox balance and proposes these biomarkers for the evaluation of knee joint pathologies.
Glutathione level of Desulfovibrio desulfuricans ІМV K-6 under the influence of heavy metal salts
I. V. Kushkevych, S. O. Hnatush, H. V. Mutenko
Ivan Franko Lviv National University, Ukraine;
e-mail: Ivan_Kushkevych@ukr.net
Glutathione is the metal stress protector and changes of its level in the sulfate-reducing bacteria cells under the influence of heavy metal salts have not been studied yet. CdCl2, Pb(NO3)2, CuCl2, and ZnCl2 influence on the total glutathione level in cell-free extracts of sulfate-reducing bacteria Desulfovibrio desulfuricans ІМV K-6 was studied. The research has been carried out using Ellman, Lowry methods, statistical processing of the results. It was shown that the glutathione level depends on the heavy metal salts concentration in the medium. The total glutathione level was the highest under the influence of Pb(NO3)2. Other salts were also toxic to bacteria because glutathione level increased in bacterial cells after addition of these salts to the medium. On the basis of the results of our work the range of heavy metal salts influence on D. desulfuricans ІМV K-6 cells glutathione level has been formed for the first time: Pb(NO3)2 > CuCl2 > CdCl2 > ZnCl2.
Metal-binding functions and antioxidant properties in human thyroid gland under iodine deficient nodular colloidal goiter
H. I. Falfushynska1, L. L. Gnatyshyna1, D. V. Osadchuk2, V. O. Shidlovski2, О. B. Stoliar1
1Volodymyr Hnatiuk Ternopil National Pedagogical University, Ukraine;
2Horbachevski Ternopil State Medical University, Ukraine;
e-mail: halynka.f@gmail.com
Copper and zinc levels in the tissue of thyroid gland (TG) and their metal-binding proteins metallothioneins (MT) as well as state of the antioxidant system in persons that had no thyroid disease and patients with endemic iodine deficiency nodular colloidal goiter has been investigated. In the patients with thyroid disease, oxidative damage was indicated despite elevated levels of MT-SH and glutathione, and elevated copper and decreased zinc concentration in TG tissue. MTs partly bound the excess of copper but its concentration in the unbound to MT form was two-fold compared to the control value.
Metal-binding capacity of metallothioneins of the liver of rats poisoned with heavy metals
B. O. Tsudzevich, I. V. Kalinin
Taras Shevchenko Kyiv National University, Ukraine;
е-mail: ikalin@rambler.ru
The functioning of metallothioneins in the liver of rats, poisoned with copper sulfate and cadmium sulfate has been investigated. By sequential chromatography on sephadex G-50 and DEAE-cellulose the authors obtained metallothioneins (MT-1, MT-1A, MT-2, MT-2a), which differ in molecular weight and composition of associated metals. Heavy metal poisoning leads to activation of synthesis and metal-binding function of metallothioneins, as well as to changes in the composition of their isoforms.
Functions of metallothioneins and a system of antioxidant defense under the effect of Co- and Zn-containing nanocomposites on crucian carp (Carassius auratus gibelio)
H. I. Falfushynska1,2, L. L. Gnatyshyna1, O. O. Turta1, O. B. Stoliar1,
N. E. Mitina3, O. S. Zaichenko3, R. S. Stoika4
1Volodymyr Hnatiuk Ternopil National Pedagogical University, Ukraine;
2I. Ya. Horbachevski Ternopil State Medical University, Ukraine;
e-mail: halynka.f@gmail.com;
3Lviv Polytechnic National University, Ukraine;
4Institute of Cell Biology, National Academy of Sciences of Ukraine, Lviv
The effect of metal-nanocomposites (Me-NC) of cobalt and zinc (Co- and Zn-NC, correspondingly) synthecized on the basis of vinylpyrrolidone (PS) on the metal-accumulative proteins with antioxidant potential metallothioneins (MT) in crucian carp (Carassius auratus gibelio) was studied. Fish was subjected to the effect of Co-NC, Zn-NC, Co2+, Zn2+ or polymer carrier (PC) in the concentrations correspondent to 50 µg×Co/l or 100 µg×Zn/l during 14 days. It was shown that the MTs response is highly specific for the nature of metal, both in ion and Me-NC form: the effect of Co and Co-NC provoked the elevation of total MT concentration (MT-SH) and activation of antioxidant defence, whereas Zn and Zn-NC induced the decrease of the concentration of MT-SH and the inhibition of antioxidant defense. All the exposures provoked the decrease of the concentration of immunoreactive chelating MT form (MTi) and reduced glutathione, activation of anaerobiosys and Mn-superoxide dismutase, and also decrease of the concentration of proteins and lipids oxidative injury products. It was accompanied by the increase of the content of erythrocytes with nuclear abnormalities but did not cause the decrease of choline esterase activity. According to the rate of MT-SH and MTi concentrations, antioxidant potential of MTs is determined by its apoform. Our data indicate that partial biodegradation of Me-NC occurs in the organism of crucian carp.
Manifestations of oxidative stress and molecular damages in ovarian cancer tissue
H. I. Falfushynska1,2, L. L. Gnatyshyna1,2, H. V. Deneha1, O. Y. Osadchuk1, O. B. Stoliar1
1Volodymyr Hnatiuk Ternopil National Pedagogical University, Ukraine;
e-mail: halynka.f@gmail.com;
2I.Ya. Horbachevsky Ternopil State Medical University, Ukraine
Indices of oxidative stress are recognized molecular markers and prognostic criteria for malignant transformation of tissue, but their value depends on the type of tumor and the stage of its development. The goal of this study was to clarify the relationship between the characteristics of the oxidative stress system including metal-associated ones and the cytotoxicity manifestations in neoplastically transformed human ovarian tissue. The highest level of Mn-superoxide dismutase activity (by 630%) and metallothionein protein (MT, 100%) has been estimated for the first time in malignant ovarian tissue compared to normal ovarian tissue. The researchers have also found a much higher level of oxy-radical formation (by 332%), a lower activity of catalase (by 49%) and a lower level of reduced glutathione (by 46%) and its redox index (0.84 versus 0.89 in the control) in tumor tissue. Under the relatively stable content of zinc, copper and cadmium in MTs, the content of zinc and especially copper in a form non-binding with MTs was significantly lower in the malignant tissue compared to normal one while the content of cadmium was higher. A discriminant analysis of all definable parameters revealed that the higher content of the products of oxidative destruction of proteins, lipids, fragmented DNA and the activity of cathepsin D, especially in its free form (by 235%), are the main characteristic signs of malignant ovarian tissue.
Trace elements storage peculiarities and metallothionein content in human thyroid gland under iodine deficiency euthyroid nodular goiter
H. I. Falfushynska1,2, L. L. Gnatyshyna1, O. Osadchuk2, V. O. Shidlovski2, О. B. Stoliar1
1Ternopil Volodymyr Hnatiuk National Pedagogical University, Ukraine;
2I.Ya. Horbachevski Ternopil State Medical University, Ukraine;
e-mail: halynka.f@gmail.com
Accumulation of iodine and copper in the node, paranodular and contralateral (not affected tissue by node) tissues of thyroid gland in relation to the level of metal-binding proteins, potential antioxidants and oxidative changes in tissue was investigated. To assess the severity of the pathological process the molecular markers of cytotoxicity were used. The reduction of total iodine (by 19.5%), increase of inorganic iodine fraction (by 82.4%) and total copper content (twice) in paranodular and nodular tissues compared with contrlateral part have been established. Excess of copper in goitrous-changes tissue was partially accumulated in the metallothioneins. The level of metal-binding form of metallothioneins and reserve of free thiols of these proteins was higher two-three times and lower content of reduced glutathione in node-affected tissue compared to the contralateral part. Signs of cytotoxicity among them: higher cathepsine D free activity (up to 84.6% and 134.4% in paranodular tissue and node respectively) and higher level of DNA strand breaks in the node (up to 22.6%) were observed. In paranodular tissue the range of indices variability compared with parenchyma of contralateral part is shorter than in the node. Thus, under low level of iodine organification and high copper level in goitrous-modified tissue of thyroid gland metallothionein may provide a partial compensatory effect on prooxidative processes.