Ukr.Biochem.J. 2014; Volume 86, Issue 1, Jan-Feb, pp. 68-74
doi: http://dx.doi.org/10.15407/ubj86.01.068
Interaction peculiarities of polyreactive immunoglobulins and various antigens
S. A. Bobrovnik, M. O. Demchenko, S. V. Komisarenko
Palladin Institute of Biochemistry, National Academy of Sciences of Ukarine, Kyiv;
е-mail: s-bobrov@bk.ru
The influence of twin 20, lysozyme and protamine on the capability of polyreactive immunoglobulins (PRIG) to attach to various antigens was investigated. Twin 20 can inhibit the binding of PRIG to antigens on immunological plates but lysozyme and protamine can enhance it. As far as the mixture of the optimal concentrations of lysozyme and protamine cannot increase PRIG-antigen interaction in comparison to the optimal dose of protamine, we have concluded that the mechanism of their effect on PRIG binding is similar. Of special interest is the fact that twin 20 at optimal concentration of lysozyme or protamine does not decrease PRIG binding to various antigens but, on the contrary, increases PRIG-antigen interaction.
Keywords: antigen-antibody interaction, ELISA, lysozyme, polyreactive immunoglobulins, protamine, twin 20
References:
- Bobrovnik SA. Activation of “silent” antibodies and their interaction with antigens. Ukr Biokhim Zhurn. 1990 Sep-Oct;62(5):86-9. PubMed
- Bobrovnik SA, Lyashchenko KP, Komisarenko SV. Polyspecific antibodies and their activation. Dopovidi Akad. Nauk UkrSSR. 1990;(6):68-71.
- Bobrovnik SA, Marinets AV. Properties of polyreactive immunoglobulins. Ukr Biokhim Zhurn. 1993 Sep-Oct;65(5):21-6. PubMed
- Berzofsky JA, Berkover IJ, in Fundamental Immunology, 4th ed., Paul WE. Translated under the title Immunologiya, Moscow: Mir, 1989. Vol.3. P. 5–88.
- Bobrovnik SA. Polyreactive immunoglobulins recognize hydrophobic parts of proteins. Ukr Biokhim Zhurn. 2001 Mar-Apr;73(2):116-22. PubMed
- Bobrovnik SA. Mechanism of interaction between polyreactive immunoglobulins and protein antigens. Ukr Biokhim Zhurn. 2002 Mar-Apr;74(2):37-44. PubMed
- Zhou ZH, Zhang Y, Hu YF, Wahl LM, Cisar JO, Notkins AL. The broad antibacterial activity of the natural antibody repertoire is due to polyreactive antibodies. Cell Host Microbe. 2007 Mar 15;1(1):51-61. PubMed, PubMedCentral, CrossRef
- Zhou ZH, Tzioufas AG, Notkins AL. Properties and function of polyreactive antibodies and polyreactive antigen-binding B cells. J Autoimmun. 2007 Dec;29(4):219-28. Review. PubMed, PubMedCentral, CrossRef
- Xiong Y, Zhou ZH, Notkins AL. Pictorial demonstration of the simultaneous binding of multiple unrelated antigens to individual polyreactive antibody-producing B cells. Scand J Immunol. 2012 Sep;76(3):342-3. PubMed, PubMedCentral, CrossRef
- Bobrovnik SA, Ctarodub NF. New simple way to immobilize the antigens on the immune plates. Immunologia. 1988;(5):83-85.
- Bobrovnik SA. Transformation of serum immunoglobulins and monoclonal antibodies into polyreactive immunoglobulins. Ukr Biokhim Zhurn. 1997 Sep-Dec;69(5-6):97-109. PubMed
This work is licensed under a Creative Commons Attribution 4.0 International License.