Ukr.Biochem.J. 2014; Volume 86, Issue 1, Jan-Feb, pp. 93-100

doi: http://dx.doi.org/10.15407/ubj86.01.093

Mitochondrial thiol-disulfide system under acute hypoxia and hypoxic-hyperoxic adaptation

04.06.2015   Uncategorized   No comments

O. A. Gonchar, I. N. Mankovska

Bogomoletz Institute of Physiology, National Academy of Sciences of Ukraine;
e-mail: ogonchar@yandex.ru

The authors investigated the state of mitochondrial glutathione pool (reduced and oxidized glutathione, protein-GSH mixed disulfides), content of carbonyl groups and free sulfhydryl groups of proteins, protein expression of key mitochondrial antioxidant enzymes such as glutathione peroxidase and thioredoxin reductase as well as activity of glutathione reductase, glutathione peroxidase and glutaredoxin in the liver mitochondria of rats exposed to acute hypoxia after prolonged hypoxic-hyperoxic training (HHT). It was shown that the preliminary HHT reduced the intensity of proteins oxidative modification under acute hypoxia, activated the mitochondrial antioxidant defense as well as affected the thiol-disulfide exchange, redox balance in mitochondria, modulated the S-glutathionylation/deglutathionylation process in mitochondria membranes.

Keywords: , , , ,

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