Ukr.Biochem.J. 2014; Volume 86, Issue 4, Jul-Aug, pp. 103-109

doi: http://dx.doi.org/10.15407/ubj86.04.103

Proapoptotic properties of total phytohemagglutinine and its individual isolectins in human cell culture 4BL

T. O. Kochubei, O. V. Maksymchuk, L. L. Маcewicz, О. O. Piven, L. L. Lukash

Institute of Molecular Biology and Genetics, National Аcademy of Sciences of Ukraine, Кyiv;
е-mail: kochubei2009@ukr.net

Phytohemagglutinine (PHA) is widely investigated lectin with mitogenic properties. Recently it was shown that  PHA is not only cell proliferation inducer, but also has a toxic or cytostatic effect. However concentration dependence and molecular mecha­nisms of this effect are not enough investigated­. To study proapoptotic properties of total phytohemagglutinine and its isolectins in human cell culture of not tumor origin 4BL we observed a change in the frequency of apoptotic cells in the tested cell culture under the influence of the total phytohemagglutinine and erythroagglutinin by the method of specific color luminescent dye. The activation of caspases-3 and -8 and induction of protein Bax expression under the influence of lectins were detected by Western blot analysis. It was revealed that erythroagglutinin induced apoptosis with the highest efficiency compared with leukoagglutinin and total phytohemagglutinine. The induction of apoptosis in human cell culture of not tumor origin 4BL is probably caused by activating caspase-dependent and mitochondrial signalling.

Keywords: , , , , ,


References:

  1. Piven’ OA, Lukash LL. Influence of exogeneous proteins on mutagenic process. Tsitol Genet. 2011 Jan-Feb;45(1):68-79. Review. Russian. PubMed
  2. Kovalenko OO, Lukash LL. Effect of lectins on induction of apoptosis in the populations of mammalian cells in vitro. Tsitol Genet. 2007 Sep-Oct;41(5):48-53. Ukrainian. PubMed
  3. Antonyuk V. O. Lectins and theirs sources of raw materials. Lviv: Kvart, 2005. 554 p. (In Ukrainian).
  4. Baintner K, Kiss P, Bardocz S, Pusztai A. Effect of orally administered plant lectins on intestinal liquor accumulation and amylase activity in rats. Acta Physiol Hung. 2004;91(1):73-81. PubMed, CrossRef
  5. Kuo WT, Ho YJ, Kuo SM, Lin FH, Tsai FJ, Chen YS, Dong GC, Yao CH. Induction of the mitochondria apoptosis pathway by phytohemagglutinin erythroagglutinating in human lung cancer cells. Ann Surg Oncol. 2011 Mar;18(3):848-56. PubMed, CrossRef
  6. Lis H, Sharon N. Lectins: Carbohydrate-Specific Proteins That Mediate Cellular Recognition. Chem Rev. 1998 Apr 2;98(2):637-674. PubMedCrossRef
  7. Peumans WJ, Hao Q, Van Damme EJ. Ribosome-inactivating proteins from plants: more than RNA N-glycosidases? FASEB J. 2001 Jul;15(9):1493-506. Review. PubMed, CrossRef
  8. Lukash LL, Yatsyshyna AP, Kushnyruk VA, Pydpala OV. Changing of adult somatic cells in vitro. Proceedings of the International Conference “Factors Experimental evolution of organisms”. Alushta, September 2011. K.: Logos, 2011. P. 493–498.
  9. Lukash LL, Paton EB, Sukhorada EM, Ruban TA, Krupskaia IS, Kostetskaia EV, Bratsiuk SI. The evaluation of the cytotoxicity of preparations with anticarcinogenic effect in cultures of human cell. Tsitol Genet. 1997 Nov-Dec;31(6):26-34. Russian. PubMed
  10. McGanon AJ, Martin SJ, Bissonnette RP, Mahboubi A, Shi Y, Mogil RJ, Nishioka WK, Green DR. The end of the (cell) line: methods for the study of apoptosis in vitro. In book Methods in cell biology. Schwartz LM, Osborne BA, editors. Acad. Pres Inc “Cell Death”, 1995. P. 172–173.
  11. Maksymchuk OV, Bezdrobna LK, Sidorik LL, Kiseleva OK, Chaschyn MO. Cytochrome P450 2E1 expression in mice liver under exposure of continuous and acute γ-radiation. Ukr Biokhim Zhurn.  2008 Jul-Aug;80(4):59–65. (In Ukrainian). PubMed
  12. Laemmli UK. Cleavage of structural proteins during the assembly of the head of bacteriophage T4. Nature. 1970 Aug 15;227(5259):680-5. PubMed, CrossRef
  13. Kоchubei ТO, Piven ОO, Аndrienko VI, Маcewicz LL, Karpova IS, Lukash LL. Influence of Phaseolus vulgaris phytohemag­glutinin and its isoforms on the proliferation and survival of mammalian cells in vitro. The Bulletin of Vavilov Society of Geneticists and Breeders of Ukraine. 2012;10(1):42–50. (In Ukrainian).
  14. Wang D, Liang J, Zhang Y, Gui B, Wang F, Yi X, Sun L, Yao Z, Shang Y. Steroid receptor coactivator-interacting protein (SIP) inhibits caspase-independent apoptosis by preventing apoptosis-inducing factor (AIF) from being released from mitochondria. J Biol Chem. 2012 Apr 13;287(16):12612-21. PubMed, PubMedCentral, CrossRef
  15. Lima LR, Bezerra MF, Almeida SM., Silva LP, Beltrão EI, Carvalho Júnior LB. Glycophenotype evaluation in cutaneous tumors using lectins labeled with acridinium ester. Dis Markers. 2013;35(3):149-54.  PubMed, PubMedCentral, CrossRef
  16. Eriksson D, Löfroth PO, Johansson L, Riklund K, Stigbrand T. Apoptotic signalling in HeLa Hep2 cells following 5 Gy of cobalt-60 gamma radiation. Anticancer Res. 2009 Nov;29(11):4361-6. PubMed
  17. Yamamoto H, Swoger J, Greene S, Saito T, Hurh J, Sweeley C, Leestma J, Mkrdichian E, Cerullo L, Nishikawa A, Ihara Y, Taniguchi N, Moskal JR. Beta1,6-N-acetylglucosamine-bearing N-glycans in human gliomas: implications for a role in regulating invasivity. Cancer Res. 2000 Jan 1;60(1):134-42. PubMed

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