Ukr.Biochem.J. 2014; Volume 86, Issue 5, Sep-Oct, pp. 142-150


The activity of prooxidant-antioxidant system in loach embryos under the action of microwave radiation

M. M. Yaremchuk, M. V. Dyka, D. I. Sanagursky

Ivan Franko National University of Lviv,Ukraine;

Electromagnetic radiation (EMR) affects biological organisms, primarily on the cellular level. However, the effects of EMR at low-intensity exposure on animals and state of metabolic systems are not fully defined yet. Thus, research of microwave radiation influence on the processes of lipid peroxidation and antioxidant protection system is important for understanding the mechanisms of EMR action on the cell, in particular, and organism development on the whole. The content of lipid peroxidation products – lipid hydroperoxides, thiobarbituric acid reactive substances and the activity of antioxidant enzymes – superoxide dismutase, glutathione peroxidase and catalase in loach embryos under the action of microwave radiation (GSM-900 MHz, SAR = 1.1 Vt/kg) lasting 1; 5; 10 and 20 min during early embryogenesis were studied. It has been found that content of lipid peroxidation products in germ cells undergoes significant changes under the action of low-intensity EMR. The effect of microwave radiation (1, 5, 10 min) leads to the increase of superoxide dismutase activity, nevertheless, 20 min exposure decreased this index to the level of control values as it is shown. It has been established that EMR at frequencies used for mobile communications reduce the activity of antioxidant protection system components, especially catalase and glutathione peroxidase. The growth of catalase activity at the 10-cell stage of blastomere division (P < 0.05) is an exception. The results of two-way analysis of variance attest that microwave radiation factor causes the large part of all observable modifications.

Keywords: , , , , ,


  1. Martynyuk VS, Tseyslyer YuV, Temu­ryants NA. Interference of the mechanisms of influence that weak extremely low-frequency electromagnetic fields have on the human body and animals. Izvestiya, Atmospheric and Oceanic Physics. 2012;48(8):832-846. CrossRef
  2. Kesari KK, Siddiqui MH, Meena R, Verma HN, Kumar S. Cell phone radiation exposure on brain and associated biological systems. Indian J Exp Biol. 2013 Mar;51(3):187-200. Review. PubMed
  3. Kesari KK, Kumar S, Behari J. Effects of radiofrequency electromagnetic wave exposure from cellular phones on the reproductive pattern in male Wistar rats. Appl Biochem Biotechnol. 2011 Jun;164(4):546-59. PubMed, CrossRef
  4. Kesari KK, Kumar S, Nirala J, Siddiqui MH, Behari J. Biophysical evaluation of radiofrequency electromagnetic field effects on male reproductive pattern. Cell Biochem Biophys. 2013 Mar;65(2):85-96. Review. PubMedCrossRef
  5. Shahin S, Singh VP, Shukla RK, Dhawan A, Gangwar RK, Singh SP, Chaturvedi CM. 2.45 GHz microwave irradiation-induced oxidative stress affects implantation or pregnancy in mice, Mus musculus. Appl Biochem Biotechnol. 2013 Mar;169(5):1727-51. PubMedCrossRef
  6. De Iuliis GN, Newey RJ, King BV, Aitken RJ. Mobile phone radiation induces reactive oxygen species production and DNA damage in human spermatozoa in vitro. PLoS One. 2009 Jul 31;4(7):e6446. PubMed, PubMedCentralCrossRef
  7. Güler G, Tomruk A, Ozgur E, Sahin D, Sepici A, Altan N, Seyhan N. The effect of radiofrequency radiation on DNA and lipid damage in female and male infant rabbits. Int J Radiat Biol. 2012 Apr;88(4):367-73. PubMed, CrossRef
  8. Grigor’ev IuG. Biological effects of mobile phone electromagnetic field on chick embryo (risk assessment using the mortality rate). Radiats Biol Radioecol. 2003 Sep-Oct;43(5):541-3. Russian. PubMed
  9. Yakymenko IL, Henshel D, Sidorik EP, Tsybulin AS, Rozumnjuk VT. Effect of mobile phone electromagnetic radiation on somitogenesis of birds. Rep. National Acad. Sci. Ukraine. 2011;(1):146-152. (In Russian).
  10. Khorseva NI. Ecological significance of natural electromagnetic fields during the prenatal human period. N. I. Khorseva. Dissertation. … Candidate of Biological Sciences. Moscow, 2004. 144 p. (In Russian).
  11. Marino AA, Carrubba S, Frilot C, Chesson AL Jr. Evidence that transduction of electromagnetic field is mediated by a force receptor. Neurosci Lett. 2009 Mar 13;452(2):119-23. PubMed, CrossRef
  12. Neifach AА. Molecular biology of developmental processes. Moscow: Nauka, 1977. 311 р. (In Russian).
  13. Lowry OH, Rosebrough NJ, Farr AL, Randall RJ. Protein measurement with the Folin phenol reagent. J Biol Chem. 1951 Nov;193(1):265-75. PubMed
  14. Mironchik V. V. Method of determination of lipid hydroperoxides in biological tissues. Patent SU, no. 1084681. 1984. (In Russian).
  15. Timirbulatov RA, Seleznev EI. Method for increasing the intensity of free radical oxidation of lipid-containing components of the blood and its diagnostic significance. Lab Delo. 1981;(4):209-11. Russian. PubMed
  16. Kostiuk VA, Potapovich AI, Kovaleva ZhV. A simple and sensitive method of determination of superoxide dismutase activity based on the reaction of quercetin oxidation. Vopr Med Khim. 1990 Mar-Apr;36(2):88-91. Russian. PubMed
  17. Koroliuk MA, Ivanova LI, Mayorova IG, Tokarev VE. A method of determining catalase activity. Lab Delo. 1988;(1):16-9. Russian. PubMed
  18. Moin VM. A simple and specific method for determining glutathione peroxidase activity in erythrocytes. Lab Delo. 1986;(12):724-7. Russian. PubMed
  19. Sanagursky DI. Objects of Biophysics: Monograph. Lviv: Publishing Center of Ivan Franko National University of Lviv, 2008. 522 p. (In Russian).
  20. Poberezkina NB, Osinskaya LF. The biological role of superoxide dismutase. Ukr Biokhim Zhurn. 1989 Mar-Apr;61(2):14-27. Review. Russian. PubMed

Creative CommonsThis work is licensed under a Creative Commons Attribution 4.0 International License.