Tag Archives: superoxide dismutase

Preventive effect of N-stearoylethanolamine on memory disorders, blood and brain biochemical parameters in rats with experimental scopolamine-induced cognitive impairment

T. M. Horid’ko1, H. V. Kosiakova1, A. G. Berdyshev1, O. F. Meged1,
O. V. Onopchenko1, V. M. Klimashevsky1, О. S. Tkachenko1, V. R. Bazylianska1,
V. O. Kholin2, K. O. Peschana2, S. A. Mykhalskiy2, N. M. Hula1

1Palladin Institute of Biochemistry, National Academy of Sciences of Ukraine, Kyiv;
2Institute of Gerontology, National Academy of Medical Sciences of Ukraine, Kyiv;
e-mail: TanGoRi@ukr.net

The impairment of cognitive functions is the most studied medical and social problem nowadays. The aim of this study was to evaluate the protective effects of N-stearoylethanolamine (NSE) on memory state, blood and brain biochemical parameters in rats under scopolamine-induced cognitive impairment. The results of this study shown that NSE administration to rats per os (5 mg/kg, 5 days, during last 3 days NSE was administrated 20 min prior to scopolamine injection (1 mg/kg, once daily for 3 days, intraperitoneally)) prevented the development of memory impairment. In particular, NSE action was associated with the prevention of increase in acetylcholinesterase activity, changes in phospholipid, free and esterified cholesterol level in hippocampus and frontal cortex, and disruption in pro-/antioxidant balance in blood and studied brain sections. Considering the above mentioned biological effects, NSE is a promising drug candidate for integrative therapy of cognitive impairment of different profiles.

Antioxidative effect of the N-stearoylethanolamine in the heart tissue and blood plasma of rats under doxorubicin treatment

I. A. Goudz, N. M. Gula, T. O. Khmel, T. M. Goridko, A. G. Berdyshev

Palladin Institute of Biochemistry, National Academy of Sciences of Ukraine, Kyiv;
e-mail: ngula@biochem.kiev.ua

The influence of N-stearoylethanolamine on the alterated antioxidant enzyme activity in the heart tissue and blood plasma of rats under the doxorubicin treatment was investigated.  It was shown that doxorubicin administration caused the decrease of antioxidant enzymes activity (superoxide dismutase and glutathione peroxidase) in the heart tissue. Administration of the NSE promoted the partial normalization of these enzymes activity. It was shown that doxorubicin treatment caused the increase of the urea and creatinine level in the blood plasma of experimental animals.  The NSE administration normalized the level of the urea and did not affect creatinine level.

Influence of salicylic and succinic acids on formation of active oxygen forms in wheat coleoptiles

Yu. Ye. Kolupaev, T. O. Yastreb, M. V. Shvidenko, Yu. V. Karpets

V. V. Dokuchaev Kharkiv National Agrarian University, Ukraine;
e-mail: plant_biology@mail.ru

The comparative study of influence of exogenous salicylic (SaA) and succinic (SuA) acids on the production of reactive oxygen species by isolated wheat coleoptiles has been provided. Under the action of both acids the increase of generation of superoxide anion-radical (O2•–) was observed. This increase was partially suppressed by treatment of coleoptiles with inhibitors of peroxidase (salicylhydroxamic acid) and NADP·H-oxidase (imidazole and α-naphthol). The increase of hydrogen peroxide content, activity of peroxidase and superoxide dismutase (SOD) was registered under the influence of SaA and SuA; catalase activity did not change essentially. The treatment of coleoptiles with the indicated acids resulted in the increase of their resistance to abiotic stress (damaging heating, 43±0.1 °С, 10 min). The conclusion is made, that the increase of O2•– generation in wheat coleoptiles under the action of SaA and SuA is related, probably, to the increase of apoplast peroxidase and NADP·H-oxidase activity, and the rise of H2O2 content is related to the growth of SOD activity. These enzymatic systems are involved in the induction of plant cells protective reactions to the hyperthermia.

Tissue specificity of antioxidant system functioning and lipid peroxidation in different age groups of amur carp

S. I. Kras, S. I. Tarasjuk

Institute of Fisheries, National Academy of Agrarian Sciences of Ukraine, Kyiv;
e-mail: s_kras@inbox.ru, tarasjuk@ukr.net

Key features of tissue enzymes functioning in antioxidant system (AOS) in sexually mature and immature individuals of Amur carp were studied. The activity of antioxidant enzymes was highest in the myocardium and subjected to age-related changes. It was concluded that changes in the functioning of AOS and intensity of lipid peroxidation processes are characterized by organ-tissue metabolic features and age peculiarities of metabolism that is most expressed in the myocardium.

Induction of plant cells heat resistance by hydrogen sulfide donor is mediated by H(2)O(2) generation with participation of NADPH oxidase and superoxide dismutase

Yu. E. Kolupaev1,2, E. N. Firsova1, Т. О. Yastreb1

1V.V. Dokuchaev Kharkiv National Agrarian University, Ukraine;
e-mail: plant_biology@ukr.net;
2V.N. Karazin Kharkiv National University, Ukraine

The participation of enzymatic systems carrying out generation and conversion of reactive oxygen species (ROS), in realization of the stress-protective effect of hydrogen sulfide (H2S) on wheat coleoptile cells was investigated. It has been shown that the treatment of isolated coleoptiles with a 100 μM hydrogen sulfide donor sodium hydrosulfide (NaHS) caused a transient enhancement of the generation of superoxide anion radical (O2•–), an increase of hydrogen peroxide content and superoxide dismutase (SOD) activity in them. The increase in ROS generation was eliminated by the inhibitor of NADPH oxidase imidazole, but not by the peroxidase inhibitor sodium azide. Treatment of coleoptiles with SOD inhibitor sodium diethyldithiocarbamate (DDC) enhanced the generation of O2•– and neutralized the effect of increasing H2O2 content induced by NaHS. One day after treatment with the H2S donor, the generation of ROS decreased to a control level, while the activity of antioxidant enzymes increased markedly and the resistance of coleoptiles to damaging heating­ was increased. These effects of the hydrogen sulfide donor were eliminated by coleoptiles’ treatment with inhibitors of NADPH oxidase (imidazole) and SOD (DDC). It was concluded that both NADPH oxidase, genera­ting O2•– , and SOD, which turns it into H2O2 performing signaling functions, are involved in the formation of a signal that induces protective systems and causes an increase in heat resistance of plant cells.

Changes in oxidative stress intensity in blood of tumor-bearing rats following different modes of administration of rhenium-platinum system

K. L. Shamelashvili1, N. I. Shtemenko2, І. V. Leus3, S. O. Babiy4, O. V. Shtemenko5

 1SE “Dnipropetrovsk Medical Academy” of Health Ministry of Ukraine;
2Palladin Institute of Biochemistry, National Academy of Sciences of Ukraine, Kyiv;
3University of Oklahoma, Oklahoma-city, USA;
4Institute of Gastroenterology, National Academy of Medical Sciences
of Ukraine, Dnipropetrovsk;
5Ukrainian State University of Chemical Technology, Dnipropetrovsk;
e-mail: shamelashvili@rambler.ru

Effects of the different modes of administration of dichlorotetra-μ-isobutyratodirhenium(ІІІ) – І – (in water solution, liposomes, nanoliposomes and together with cisplatin – in the rhenium-platinum system) on the intensity of lipid peroxidation (LP) in blood plasma and the activity of the erythrocyte antioxidant enzymes were investigated on the model of tumor growth. A decrease in the concentration of TBA-active substances caused by dirhenium compounds was shown to be independent of the administration mode and the extent of the tumor growth inhibition. I was four-times more effective in inhibition of the LP burst than any known antioxidant. I induced the increasing activity of erythrocyte superoxide dismutase and decreasing activity of catalase. In vitro experiments with native superoxide dismutase, the interaction of І with following activation of the active center of the enzyme was confirmed and the superoxide dismutase activity of І was shown, that may contribute to the enhancement of the enzyme activity in vivo. The cluster rhenium compounds may be promising nontoxic potent antioxidants capable of deactivating superoxide radicals.

Loach embryos prooxidant-antioxidant status under the influence of amide derivatives of 1,4-naphthoquinone

A. O. Bezkorovaynyj1,2, A. R. Zyn2, N. M. Harasym1, J. T. Len2,3, O. M. Figurka3, D. I. Sanagursky1

1Ivan Franko National University of Lviv, Ukraine;
2Lviv Expert Center of Scientific Researches Ministry of Internal Affairs of Ukraine;
3National University “Lviv Polytechnicа”, Ukraine;
e-mail: andriy.bezkorovajnyj@gmail.com

The mechanisms of disorders in cell functions induced by 1,4-naphthoquinone amide derivatives are not clarified yet. The article is dedicated to the study of features of these substances influence on loach Misgurnus fossilis L. embryos pro/antioxidant homeostasis during early embryogenesis. The aim of this work was to study the effect of 2-chloro-3-hydroxy-1,4-naphthoquinone, 2-chloro-3-(3-oxo-3-(piperidine-1-yl)propylamine)-1,4-naphthoquinone (FO-1), 2-chloro-3-(3-(morpholine-4-yl)-3-oxopropylamine)-1,4-naphthoquinone (FO-2 at concentrations of 10-3, 10-5, 10-7 M on the content of TBA-reactive substances (a byproduct of lipid peroxidation) and the activities of superoxide dismutase and catalase in loach embryos. It was established that 1,4-naphthoquinone amide derivatives and 2-chloro-3-hydroxy-1,4-naphthoquinone decreased the content of lipid peroxidation products in embryo cells in a dose-dependent manner. The investigated compounds cause an increase in superoxide dismutase and catalase activities compared with the control value. The results of the two-factor ANOVA test indicate that 2-chloro-3-hydroxy-1,4-naphthoquinone and 1,4-naphthoquinone amide derivatives (FO-1, FO-2) have predominant influence on the TBA-reactive substances content and superoxide dismutase activity. However, the time of loach embryos development has a more pronounced effect on catalase activity than the studied 1,4-naphthoquinone derivatives.

Intensity of peroxidation processes and activity of antioxidant enzymes in rat tissues at high chromium level in the diet

R. Ya. Iskra, V. G. Yanovych

Institute of Animal Biology, National Academy of Agrarian Sciences, Lviv, Ukraine;
e-mail: ruslana_iskra@inenbiol.com.ua

The data on the influence of chromium in different tissues of rats at its consumption with mixed fodder in the form of CrCl3×6H2O on the intensity of peroxidation processes and activity of antioxidant enzymes are presented. The degree of high chromium content in the studied tissues of rats at its addition to mixed fodder in the amount of 200 µg/kg during 30 days was established. Chromium content in the rat tissues decreased in the order: the spleen, heart, kidneys, lungs, brain, liver, skeletal muscle. In all tissues of rats fed with mixed fodder with chromium addition, except for skeletal muscles, content of lipid peroxidation products – hydroperoxide and TBARS-products decreased. The content of lipid peroxidation products decreased in the spleen, kidneys, liver and lungs. Also in all organs and tissues of rats the activity of glutathione peroxidase, glutathione reductase and catalase increased at the action of chromium. In the brain and kidneys the level of reduced glutathione increased. Superoxide dismutase activity was significantly higher not only in the heart and skeletal muscles of animals and is probably equal in the lungs and liver, and in other organs – the brain, kidneys and spleen in animals of the studied group the enzyme activity was lower as compared to animals of the control group. Obtained results demonstrate the regulatory influence of chromium on free radical process in the rat tissues.

Participation of the active oxygen forms in the induction of ascorbate peroxidase and guaiacol peroxidase under heat hardening of wheat seedlings

Yu. E. Kolupaev, O. I. Oboznyi

V. V. Dokuchayev Kharkiv National Agrarian University, Ukraine;
e-mail: plant_biology@mail.ru

The influence of one-minute hardening heating at 42 °C on the dynamics of hydrogen peroxide generation and activity of antioxidant enzymes in roots of winter wheat seedlings has been investigated. It was shown that the content of hydrogen peroxide increased within the first 30 minutes after heat influence, whereupon it approached the level of control variant. The activity of superoxide dismutase (SOD) increased significantly within 10 min after heating and was maintained at a high level during 24 hours of observation. The activi­ty of ascorbate peroxidase and guaiacol peroxidase increased after 3-6 hours after the hardening and reached its maximum after 24 hours, when there was the most significant increase in heat resistance of seedlings. The short-term increase in hydrogen peroxide content caused by hardening heating was suppressed by treatment of seedlings with H2O2 scavenger dimethylthiourea, inhibitors of NADPH-oxidase (imidazole) and SOD (sodium diethyldithiocarbamate). All these effectors levelled the increase of activity of ascorbate peroxidase and guaiacol peroxidase and significantly inhibited the development of heat resistance of seedlings. The conclusion was made about the role of hydrogen peroxide produced with the participation of NADPH­-oxidase and SOD in the induction of antioxidant system by heat harde­ning of wheat seedlings.

Antioxidant and antitumor activity of dirhenium dicarboxylates in animals with Guerin carcinoma

I. V. Leus1, K. L. Shamelashvili1, O. D. Skorik1, S. Y. Tretyak2,
O. A. Golichenko2, O. V. Shtemenko2, N. I. Shtemenko1

1Oles Gonchar Dnipropetrovsk National University, Ukraine;
2Ukrainian State University of Chemical Technology, Dnipropetrovsk;
e-mail: ingaleus@mail.ru

The antioxidant and anticancer properties of dirhenium dicarboxylates of cis- and trans-configuration with different organic ligands in a model of tumor growth (Guerin carcinoma) were studied. It was shown that compounds of different configuration had similar antitumor effect, and dirhenium (III) cis-dicarboxylates were characterized by higher antioxidant activity and degree of activation of erythrocyte superoxide dismutase (SOD) in comparison with trans-isomers. The dependence between the structure of dirhenium (III) dicarboxylates and their ability to activate erythrocyte SOD in the model of tumor growth was shown for the first time. The in vitro studies have shown that rhenium compounds of cis- and trans-configuration interacted similarly with erythrocyte SOD, changing the protein secondary structure. In contrast to trans-dicarboxylate, for cis-dicarboxylate the SOD-like activity was demonstrated to be on the first minutes of the xantine-oxidase reaction. The studied features of the interaction between rhenium compounds and SOD in vitro explain only partly the activation of SOD in experiments in vivo. The attempt is made to explain the differences in the mechanisms of antioxidant activity of dirhenium cis- and trans-dicarboxylates.