Ukr.Biochem.J. 2014; Volume 86, Issue 6, Nov-Dec, pp. 119-128

doi: https://doi.org/10.15407/ubj86.06.119

The effect of N-stearoylethanolamine on cholesterol content, fatty acid composition and protein carbonylation level in rats with alimentary obesity-induced insulin resistance

O. V. Onopchenko, G. V. Kosiakova, E. F. Meged,
V. M. Klimashevsky, N. M. Hula

Palladin Institute of Biochemistry, National Academy of Sciences of Ukraine, Kyiv;
e-mail: onop.89.av@mail.ru

The effect of N-stearoylethanolamine (NSE) on liver free fatty acid composition, cholesterol content and carbonylated protein level in rats with obesity-induced insulin resistance (IR) was studied in the work. The experimental insulin resistance was induced by prolonged high fat diet (58% of energy derived from fat) for 6 months combined with one injection of low-dose (15 mg/kg) of streptozotocin. The lipid assay showed a rise in liver free cholesterol content and a significant reduction in cholesterol esters level. Analyzing liver fatty acid composition, a decrease in polyunsaturated of fatty acid (PUFA) level and an increase in monounsaturated fatty acid (MUFA) content was found. Fatty acid imbalance with high content of MUFA was associated with elevated level of protein carbonylation.  The NSE administration (50 mg/kg of body weight) for 2 weeks decreased free cholesterol content, increased cholesterol esters level and reduced free oleic fatty acid content in the liver of rats with IR. The effect of NSE on lipid imbalance led to a decrease in protein carbonylation level that may result in improvement of transmembrane protein function under obesity-induced insulin resistance state.

Keywords: , , , , , ,


References:

  1. Dandona P, Ghanim H, Chaudhuri A, Dhindsa S, Kim SS. Macronutrient intake induces oxidative and inflammatory stress: potential relevance to atherosclerosis and insulin resistance. Exp Mol Med. 2010 Apr 30;42(4):245-53. Review.  PubMed, PubMedCentral, CrossRef
  2. Mlinar B, Marc J. New insights into adipose tissue dysfunction in insulin resistance. Clin Chem Lab Med. 2011 Sep 6;49(12):1925-35. Review. PubMed, CrossRef
  3. Yuzefovych LV, Musiyenko SI, Wilson GL, Rachek LI. Mitochondrial DNA damage and dysfunction, and oxidative stress are associated with endoplasmic reticulum stress, protein degradation and apoptosis in high fat diet-induced insulin resistance mice. PLoS One. 2013;8(1):e54059. PubMed, PubMedCentral, CrossRef
  4. Turk Z. Glycotoxines, carbonyl stress and relevance to diabetes and its complications. Physiol Res. 2010;59(2):147-56. PubMed
  5. Curtis JM, Hahn WS, Long EK, Burrill JS, Arriaga EA, Bernlohr DA. Protein carbonylation and metabolic control systems. Trends Endocrinol Metab. 2012 Aug;23(8):399-406. Review. PubMed, PubMedCentral, CrossRef
  6. Matteucci E, Biasci E, Giampietro O. Advanced oxidation protein products in plasma: stability during storage and correlation with other clinical characteristics. Acta Diabetol. 2001 Dec;38(4):187-9. PubMed, CrossRef
  7. Griffin ME, Marcucci MJ, Cline GW, Bell K, Barucci N, Lee D, Goodyear LJ., Kraegen EW, White MF, Shulman GI. Free fatty acid-induced insulin resistance is associated with activation of protein kinase C theta and alterations in the insulin signaling cascade. Diabetes. 1999 Jun;48(6):1270-4. PubMed, CrossRef
  8. Kosiakova HV, Hula NM. The N-stearoylethanolamine effect on the NO-synthase way of nitrogen oxide formation and phospholipid composition of erythrocyte membranes in rats with streptozotocine diabetes. Ukr Biokhim Zhurn. 2007 Nov-Dec;79(6):53-59. (In Ukrainian). PubMed
  9. Goridko TM, Gula NM, Stogniy NA, Meged OF, Klimashevsky VM, Shovkun SA, Kindruk NL, Berdyshev AG. Influence of N-stearoylethanolamine on the lipid peroxidation process and lipid composition of the rat liver under acute morphine intoxication. Ukr Biokhim Zhurn. 2007 Sep-Oct;79,(5):175-185. (In Ukrainian). PubMed
  10. Onopchenko OV, Kosiakova GV, Gorid­ko TM, Berdyschev AG, Meged OF, Hula NM. The effect of N-stearoylethanolamine on the activity of antioxidant enzymes, content of lipid peroxidation products and nitric oxide in the blood plasma blood and liver of rats with induced insulin-resistance. Ukr Biokhim Zhurn. 2013 Sep-Oct;85(5):88-96. (In Ukrainian). PubMed
  11. Epps DE, Natarajan V, Schmid PC, Schmid HO. Accumulation of N-acylethanolamine glycerophospholipids in infarcted myocardium. Biochim Biophys Acta. 1980 Jun 23;618(3):420-30. PubMed, CrossRef
  12. Oliver CN, Ahn BW, Moerman EJ, Goldstein S, Stadtman ER. Age-related changes in oxidized proteins. J Biol Chem. 1987 Apr 25;262(12):5488-91. PubMed
  13. Dubinina EE, Burmistrov SO, Khodov DA, Porotov IG. Oxidative modification of human serum proteins. A method of determining it. Vopr Med Khim. 1995 Jan-Feb;41(1):24-6. Russian. PubMed
  14. Bradford MM. A rapid and sensitive method for the quantitation of microgram quantities of protein utilizing the principle of protein-dye binding. Anal Biochem. 1976 May 7;72(1-2):248-54. PubMed, CrossRef
  15. Bligh EG, Dyer WJ. A rapid method of total lipid extraction and purification. Can J Biochem Physiol. 1959 Aug;37(8):911-7. PubMed
  16. Carreau ID, Dubaco IP. Adaptation of a macro-scale methods to the micro-scale for fatty acid methyl transcsterification of biological lipid extracts. J Chromatogr. 1978;151(3):384-390. CrossRef
  17. McLauren Dorrance A., Graham D., Dominiczak A., Fraser R. Inhibition of nitric oxide synthesis increases erythrocyte membrane fluidity and unsaturated fatty acid content. Am J Hypertens. 2000 Nov;13(11):1194-202. PubMed, CrossRef
  18. Van Rooyen DM, Larter CZ, Haigh WG, Yeh MM, Ioannou G, Kuver R, Lee SP, Teoh NC, Farrell GC. Hepatic free cholesterol accumulates in obese, diabetic mice and causes nonalcoholic steatohepatitis. Gastroenterology. 2011 Oct;141(4):1393-403, 1403.e1-5. PubMed, PubMedCentral, CrossRef
  19. Kunnen S, Van Eck M. Lecithin:cholesterol acyltransferase: old friend or foe in atherosclerosis? J Lipid Res. 2012 Sep;53(9):1783-99. Review. PubMed, PubMedCentral, CrossRef
  20. Ntambi JM, Miyazaki M, Stoehr JP, Lan H, Kendziorski CM, Yandell BS, Song Y, Cohen P, Friedman JM, Attie AD. Loss of stearoyl-CoA desaturase-1 function protects mice against adiposity. Proc Natl Acad Sci USA. 2002 Aug 20;99(17):11482-6. PubMed, PubMedCentral, CrossRef
  21. Gutiérrez-Juárez R, Pocai A, Mulas C, Ono H, Bhanot S, Monia BP, Rossetti L. Critical role of stearoyl-CoA desaturase-1 (SCD1) in the onset of diet-induced hepatic insulin resistance. J Clin Invest. 2006 Jun;116(6):1686-95. PubMed, PubMedCentral, CrossRef
  22. Terrazzino S, Berto F, Dalle Carbonare M, Fabris M, Guiotto A, Bernardini D, Leon A. Stearoylethanolamide exerts anorexic effects in mice via down-regulation of liver stearoyl-coenzyme A desaturase-1 mRNA expression. FASEB J. 2004 Oct;18(13):1580-2. PubMed, CrossRef
  23. Onopchenko OV, Kosiakova GV, Goridko TM, Klimashevsky VM, Hula NM. The effect of N-stearoylethanolamine on liver phospholipid composition of rats with insulin resistance caused by alimentary obesity. Ukr Biochem J. 2014 Jan-Feb;86(1):101-110. (In Ukrainian). PubMed, CrossRef
  24. Gunnlaugsson O, Berkowitz D. Individual free fatty acids in patients with liver disease. Am J Dig Dis. 1977 Nov;22(11):1005-9. PubMed, CrossRef
  25. Kotronen A., Seppanen-Laakso T., Westerbacka J., Kiviluoto T, Arola J, Ruskeepää AL, Oresic M, Yki-Järvinen H. Hepatic stearoyl-CoA desaturase (SCD)-1 activity and diacylglycerol but not ceramide concentrations are increased in the nonalcoholic human fatty liver. Diabetes. 2009 Jan;58(1):203-8.  PubMedPubMedCentral, CrossRef
  26. Savini I, Catani MV, Evangelista D, Gasperi V, Avigliano L. Obesity-associated oxidative stress: strategies finalized to improve redox state. Int J Mol Sci. 2013 May 21;14(5):10497-538.  PubMedPubMedCentralCrossRef
  27. Espinosa A, Valenzuela R, González-Mañán D, D’Espessailles A, Guillermo Gormaz J, Barrera C, Tapia G. Prevention of liver steatosis through fish oil supplementation:correlation of oxidative stress with insulin resistance and liver fatty acid content.  Arch Latinoam Nutr. 2013 Mar;63(1):29-36. PubMed
  28. Pradenas GA, Díaz-Vásquez WA, Pérez-Donoso JM, Vásquez CC. Monounsaturated fatty acids are substrates for aldehyde generation in tellurite-exposed Escherichia coli. Biomed Res Int. 2013;2013:563756. PubMed, PubMedCentral, CrossRef
  29. Hopps E, Caimi G. Protein oxidation in metabolic syndrome. Clin Invest Med. 2013 Feb 1;36(1):E1-8. Review. PubMed
  30. Curtis JM, Hahn WS, Stone MD, Inda JJ, Droullard DJ, Kuzmicic JP, Donoghue MA, Long EK, Armien AG, Lavandero S, Arriaga E, Griffin TJ, Bernlohr DA. Protein carbonylation and adipocyte mitochondrial function. J Biol Chem. 2012 Sep 21;287(39):32967-80.  PubMed, PubMedCentral, CrossRef
  31. Aoi W, Naito Y, Tokuda H, Tanimura Y, Oya-Ito T, Yoshikawa T. Exercise-induced muscle damage impairs insulin signaling pathway associated with IRS-1 oxidative modification. Physiol Res. 2012;61(1):81-8. PubMed

Creative CommonsThis work is licensed under a Creative Commons Attribution 4.0 International License.