V. M. Riasniy, L. I. Apukhovska, N. N. Veliky, I. O. Shymanskyy,
D. O. Labudzynskyi, S. V. Komisarenko

Palladin Institute of Biochemistry, National Academy of Sciences of Ukraine, Kyiv;
e-mail: Riasniy@ukr.net

The aim of this study was to determine the effectiveness of separate and combined administration of vitamin D₃ and different forms of bis­phosphonate (disodium salt of methylenbisphosphonic acid dihydrate and alendronate) on the function of immune cells in rats with nutritional osteoporosis. It was shown that D-hypovitaminosis leads to reduced 25OHD₃, which is a biomarker for vitamin D₃ and disturbances of metabolic processes in bone tissue that correlated with osteoporosis manifestation. Immunologic disorders related to nutritional osteoporosis were accompanied by the decrease in phagocytic activity of granulocytes and impaired ability to produce bactericidal oxidants. Inhibition of B-cell immunity also occurred in pathol­gy. Thus, the present study revealed more pronounced immunomodulatory effects of vitamin D₃ on phagocytic immunity, whereas bisphosphonates were effective in improving the humoral immune protection.

Keywords: bisphosphonates, immunoglobulins, nutritional osteoporosis, phagocytosis, vitamin D3