Ukr.Biochem.J. 2016; Volume 88, Issue 3, May-Jun, pp. 78-82


The impact of hydroxycitric acid on the lipid metabolism profile under experimental insulin resistance syndrome of Syrian hamsters

A. L. Zagayko, A. I. Shkapo, V. P. Fylymonenko, T. O. Briukhanova

National University of Pharmacy, Kharkiv, Ukraine;

The syndrome of insulin resistance (IR) is one of the leading reasons for the increased risk of cardiovascular diseases and their complications. Among the key components of IR are obesity and dyslipidemia. Hydroxycitric acid (HCA), an inhibitor of a key enzyme of lipogenesis ATP citrate lyase (ACLY) is a promising obesity treatment agent. The aim of this work was to investigate the effect of HCA on lipid and lipoproteins content in the blood serum, as well as lipid content and activity of some lipid metabolism enzymes in the liver of hamsters with IR. IR was modeled by keeping animals on high-fat diet with addition of fructose. Lipid content was determined by using standard reagent kits, the level of lipoproteins, the activity of glucose 6-phosphate dehydrogenase and ACLY – spectrophotometrically, lysosomal lipase activity – fluorimetrically. Development of hyperlipidemia and atherogenic dyslipidemia, lipid accumulation in the liver, activation of lysosomal lipase and ACLY and reduction of glucose 6-phosphate dehydrogenase activity were shown under IR. The treatment by HCA reduces the manifestations of hyperlipidemia, but enhances the lipid accumulation in the liver.

Keywords: , , , ,


  1. Han TS, Lean ME. A clinical perspective of obesity, metabolic syndrome and cardiovascular disease. JRSM Cardiovasc Dis. 2016 Feb 25;5:1-13. Review. PubMed, PubMedCentral, CrossRef
  2. Wang Q, Li S, Jiang L, Zhou Y, Li Z, Shao M, Li W, Liu Y. Deficiency in hepatic ATP-citrate lyase affects VLDL-triglyceride mobilization and liver fatty acid composition in mice. J Lipid Res. 2010 Sep;51(9):2516-26. PubMed, PubMedCentral, CrossRef
  3. Zagayko AL, Briukhanova TO, Shkapo AI. Some characteristics of lipids and lipoproteinies metabolism in Syrian hamsters females of different age under the experimental metabolic syndrome. World Med Biol. 2015; 52 (3): 99-103. (In Ukrainian).
  4. Lewis GF, Carpentier A, Adeli K, Giacca A. Disordered fat storage and mobilization in the pathogenesis of insulin resistance and type 2 diabetes. Endocr Rev. 2002 Apr;23(2):201-29. PubMed, CrossRef
  5. Browning JD, Horton JD. Molecular mediators of hepatic steatosis and liver injury. J Clin Invest. 2004 Jul;114(2):147-52. Review. PubMed, PubMedCentral, CrossRef
  6. Donnelly KL, Smith CI, Schwarzenberg SJ, Jessurun J, Boldt MD, Parks EJ. Sources of fatty acids stored in liver and secreted via lipoproteins in patients with nonalcoholic fatty liver disease. J Clin Invest. 2005 May;115(5):1343-51. PubMed, PubMedCentral, CrossRef
  7. Vasques CA, Schneider R, Klein-Júnior LC, Falavigna A, Piazza I, Rossetto S. Hypolipemic effect of Garcinia cambogia in obese women. Phytother Res. 2014 Jun;28(6):887-91.  PubMed, CrossRef
  8. Hayamizu K, Ishii Y, Kaneko I, Shen M, Okuhara Y, Shigematsu N, Tomi H, Furuse M, Yoshino G, Shimasaki H. Effects of garcinia cambogia (Hydroxycitric Acid) on visceral fat accumulation: a double-blind, randomized, placebo-controlled trial. Curr Ther Res Clin Exp. 2003 Sep;64(8):551-67.  PubMed, PubMedCentral, CrossRef
  9. Wang PR, Guo Q, Ippolito M, Wu M, Milot D, Ventre J, Doebber T, Wright SD, Chao YS. High fat fed hamster, a unique animal model for treatment of diabetic dyslipidemia with peroxisome proliferator activated receptor alpha selective agonists. Eur J Pharmacol. 2001 Sep 21;427(3):285-93. PubMed, CrossRef
  10. Jurgoński A, Juśkiewicz J, Zduńczyk Z. A high-fat diet differentially affects the gut metabolism and blood lipids of rats depending on the type of dietary fat and carbohydrate. Nutrients. 2014 Feb 3;6(2):616-26. PubMed, PubMedCentral, CrossRef
  11. Prokhorov MIu, Tiunov MP, Shakalis DA. Simple colorimetric micromethod of determination of free fatty acids. Lab Delo. 1977;(9):536-6. Russian. PubMed
  12. Kolb VG, Kamyshnikov VS. Quantitative study of serum lipoproteins in healthy persons by polyacrylamide gel disc electrophoresis. Lab Delo. 1979;(1):42-4. Russian. PubMed
  13. Kaliman PA, Zagayko AL, Shalamov RV, Ganusova GV, Barannik TV, Skripnik EV, Sokolik VV, Shabi BK. Content and composition of lipoproteins of rat blood and liver and various parameters of oxidative stress during administration of cobalt chloride. Ukr Biokhim Zhurn. 1997 Sep-Dec;69(5-6):138-48. Russian. PubMed
  14. Putilina FE, Zoidze SD. Methods of biochemical research (lipid and energy metabolism). Under еd. MI. Prokhorova. Leningrad: Publishing House of Leningrad University, 1982. P. 168-172. (In Russian).
  15. Aslanidis C, Ries S, Fehringer P, Büchler C, Klima H, Schmitz G. Genetic and biochemical evidence that CESD and Wolman disease are distinguished by residual lysosomal acid lipase activity. Genomics. 1996 Apr 1;33(1):85-93. PubMed, CrossRef
  16. Wang Q, Jiang L, Wang J, Li S, Yu Y, You J, Zeng R, Gao X, Rui L, Li W, Liu Y. Abrogation of hepatic ATP-citrate lyase protects against fatty liver and ameliorates hyperglycemia in leptin receptor-deficient mice. Hepatology. 2009 Apr;49(4):1166-75. PubMed, CrossRef

Creative CommonsThis work is licensed under a Creative Commons Attribution 4.0 International License.