Ukr.Biochem.J. 2016; Volume 88, Issue 5, Sep-Oct, pp. 107-115

doi: https://doi.org/10.15407/ubj88.05.107

Measurement of methionine level with the LC-ESI-MS/MS method in schizophrenic patients

07.11.2016   Uncategorized   No comments

S. Kulaksizoglu1, B. Kulaksizoglu2, H. Y. Ellidag1, E. Eren1, N. Yilmaz1, A. Baykal3

1Central Laboratories of Antalya Education and Research Hospital,
Department of Biochemistry, Antalya, Turkey;
e-mail: sblclngrgl@yahoo.co.uk;
2Antalya Education and Research Hospital, Department of Psychiatry, Antalya, Turkey;
3Akdeniz University School of Medicine, Department of Biochemistry, Antalya, Turkey

The purpose of this study was to evaluate plasma methionine levels by using liquid chromatography electrospray ionization-tandem mass spectroscopy (LC-ESI-MS/MS) in schizophrenic patients. A twelve-point standard graph was drawn, and the recovery rate, the intra-day and inter-day coefficients of variation (CV), the limit of detection (LOD), and the limit of quantification (LOQ) were evaluated. The y and R2 values of the standard graph equation were determined as 0.011x + 0.0179 and 0.9989, respectively, and the graph remained linear until the 200 µmol/l level. The intra-day coefficients of variation of the samples (n = 10) containing 8, 28, and 58 µmol/l methionine were determined as 2.68, 3.10, and 3.79%, respectively; while their inter-day coefficients of variation were determined as 2.98, 3.19, and 3.84%. The LOD and LOQ values were determined as 0.04 and 0.1 µmol/l, respectively, while the mean recovery rates were determined as 101.7 and 99.3%. Plasma methionine values were measured as 21.5 (19.5-24,6) µmol/l for the patient group, 17.8 (16.3-20.1) µmol/l for the control group, and the difference between the two groups was statistically significant (p = 0.03). LC-ESI-MS/MS method represents a fairly sensitive, economic, and rapid analysis that requires very little sample and is suitable for measuring methionine levels in schizophrenic patients.

Keywords: , , , ,

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