Ukr.Biochem.J. 2019; Volume 91, Issue 1, Jan-Feb, pp. 65-73


HBD-2 interactions with erythrocyte membranes in vitro

M. V. Makarenko1, D. O. Semeniuk2, I. O. Starenka2, A. P. Pogribna3,
I. V. Sokol4, L. I. Martinova1, D. O. Govsieiev4

1Institute of Postgraduate Education,
Bogomolets National Medical University, Kyiv Ukraine;
2ESC Institute of Biology and Medicine,
Taras Shevchenko National University of Kyiv, Ukraine;
3Institute of Molecular Biology and Genetics, NAS of Ukraine, Kyiv;
4Kyiv City Maternity Hospital No 5

Received: 24 September  2018; Accepted: 13 December 2018

HBD-2 – a member of β-defensin family of antimicrobial peptides – is known to permeabilize cell membranes of susceptible cells, but the mechanism of such interactions is poorly understood. In our study, we have used a hemolytic model to explore the kinetic properties of HBD-2 interactions with membranes of human erythrocytes. We ran hemolytic assays with a wide range of both HBD-2 and erythrocyte concentrations, as well as varying pH values, incubation times, and osmotic strengths; each in the presence or the absence of inhibitory substances such as proteins and salts. The results show that HBD-2 cell membrane permeabilization is both dose- and time-dependent (with plateau effect observed in each case), and inversely dependent on erythrocyte concentration. HBD-2 interactions with cell membranes highly depend on pH value and the presen­ce of inhibitors but are not affected by tested osmotic strength range. Our findings suggest that interactions of HBD-2 with cell membranes are mainly electrostatic in nature and are limited by released cell content. We have developed a speculative model of such interaction based on our results.

Keywords: , ,


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