Ukr.Biochem.J. 2020; Volume 92, Issue 6, Nov-Dec, pp. 5-52


Scientists’ pursuit for SARS-COV-2 coronavirus: strategies against pandemic

S. V. Komisarenko

Palladin Institute of Biochemistry, National Academy of Sciences of Ukraine, Kyiv;

The SARS-CoV-2 coronavirus is the cause of the coronavirus disease 2019 (COVID-19), which in 2020 became pandemic and a global threat. As of January 10, 2021, 218 countries and territories have reported 90.783 million confirmed cases and 1.945 million deaths. The aim of this article is to briefly review the numerous information linked to this virus and the COVID-19 disease and to give an analysis and landscape of appropriate problems. In particular, to acquaint with information on the coronavirus biology, its origin, structure and ways of infection; on the features of COVID-19 disease, diagnostics, the use of pharmaceuticals for the disease treatment and the formation of immunity against SARS-CoV-2. Particular attention was given to the vaccines’ development and the effectiveness of anti-epidemic measures (quarantine). The use of mathe­matical modeling of the epidemic process and the prospects of quarantine ending is also discussed. Finally, the data relevant to the emerging SARS-CoV-2 variant VOC 2020 12/1 are presented with special attention to its possible impact on the virus diagnostics and vaccination.

Keywords: , , , , , , ,


  1. Reimann H.A. Landmark article Dec 24, 1938: An acute infection of the respiratory tract with atypical pneumonia. A disease entity probably caused by a filtrable virus. JAMA. 1984. 251(7): 936–944. CrossRef
  2. Severe acute respiratory syndrome.
  3. Middle East respiratory syndrome.
  4. COVID-19 Dashboard by the Center for Systems Science and Engineering (CSSE) at Johns Hopkins University (JHU).
  5. In Ukraine — the first case of coronavirus. Ukrayinska pravda. March 3, 2020. (Iin Ukrainian).
  6. Wee S.-L., McNeil D.G.Jr. China Identifies New Virus Causing Pneumonialike Illness.
  7. Lu R, Zhao X, Li J, Niu P, Yang B, Wu H, Wang W, Song H, Huang B, Zhu N, Bi Y, Ma X, Zhan F, Wang L, Hu T, Zhou H, Hu Z, Zhou W, Zhao L, Chen J, Meng Y, Wang J, Lin Y, Yuan J, Xie Z, Ma J, Liu WJ, Wang D, Xu W, Holmes EC, Gao GF, Wu G, Chen W, Shi W, Tan W. Genomic characterisation and epidemiology of 2019 novel coronavirus: implications for virus origins and receptor binding. Lancet. 2020. 395(10224): 565-574. PubMed, PubMedCentral, CrossRef
  8. Beaudette FR, Hudson CB. Cultivation of the virus of infectious bronchitis. J Am Vet Med Assoc. 1937; 90: 51-58.
  9. Tyrrell DA, Bynoe ML. Cultivation of a novel type of common-cold virus in organ cultures. Br Med J. 1965;1(5448):1467-1470.  PubMed, PubMedCentral, CrossRef
  10. Woo PC, Lau SK, Huang Y, Yuen KY. Coronavirus diversity, phylogeny and interspecies jumping. Exp Biol Med (Maywood). 2009;234(10):1117-1127. PubMed, CrossRef
  11. Derek Wong’s Virology.
  12. Phylogeny of SARS-like betacoronaviruses including novel coronavirus SARS-CoV-2.
  13.  Davidson H. First Covid-19 case happened in November, China government records show — report.
  14. Huang C, Wang Y, Li X, Ren L, Zhao J, Hu Y, Zhang L, Fan G, Xu J, Gu X, Cheng Z, Yu T, Xia J, Wei Y, Wu W, Xie X, Yin W, Li H, Liu M, Xiao Y, Gao H, Guo L, Xie J, Wang G, Jiang R, Gao Z, Jin Q, Wang J, Cao B. Clinical features of patients infected with 2019 novel coronavirus in Wuhan, China. Lancet. 2020;395(10223):497-506. PubMed, PubMedCentral, CrossRef
  15. Osborne H. Coronavirus Outbreak May Have Started As Early As September, Scientists Say.
  16. Zhou P, Yang XL, Wang XG, Hu B, Zhang L, Zhang W, Si HR, Zhu Y, Li B, Huang CL, Chen HD, Chen J, Luo Y, Guo H, Jiang RD, Liu MQ, Chen Y, Shen XR, Wang X, Zheng XS, Zhao K, Chen QJ, Deng F, Liu LL, Yan B, Zhan FX, Wang YY, Xiao GF, Shi ZL. A pneumonia outbreak associated with a new coronavirus of probable bat origin. Nature. 2020;579(7798):270-273. PubMed, PubMedCentral, CrossRef
  17. Readfearn G. How did coronavirus start and where did it come from? Was it really Wuhan’s animal market?
  18. Liu P, Jiang JZ, Wan XF, Hua Y, Li L, Zhou J, Wang X, Hou F, Chen J, Zou J, Chen J. Are pangolins the intermediate host of the 2019 novel coronavirus (SARS-CoV-2)? PLoS Pathog. 2020;16(5):e1008421. PubMed, PubMedCentral, CrossRef
  19. Andersen KG, Rambaut A, Lipkin WI, Holmes EC, Garry RF. The proximal origin of SARS-CoV-2. Nat Med. 2020;26(4):450-452. PubMed, PubMedCentral, CrossRef
  20. Shinkman PD. U.S. Spy Agencies Weigh In on Coronavirus Rumors.
  21. Sanger DE. Pompeo Ties Coronavirus to China Lab, Despite Spy Agencies’ Uncertainty.
  22. Pennisi E. How bats have outsmarted viruses — including coronaviruses — for 65 million years. Science. Jul. 22, 2020. CrossRef
  23. Scudellari M. The sprint to solve coronavirus protein structures – and disarm them with drugs. Nature. 2020;581(7808):252-255. PubMed, CrossRef
  24. Zhang L, Lin D, Sun X. et al. Crystal structure of SARS-CoV-2 main protease provides a basis for design of improved α-ketoamide inhibitors. Science. 2020. 368(6489): 409-412. CrossRef
  25. Wu F, Zhao S, Yu B, Chen YM, Wang W, Song ZG, Hu Y, Tao ZW, Tian JH, Pei YY, Yuan ML, Zhang YL, Dai FH, Liu Y, Wang QM, Zheng JJ, Xu L, Holmes EC, Zhang YZ. A new coronavirus associated with human respiratory disease in China. Nature. 2020. 579(7798): 265-269. CrossRef
  26. Schmidt A, Wolff MH, Weber O. Coronaviruses with special emphasis on first insights concerning SARS. Springer, 2005. 232 p. CrossRef
  27. Wrapp D, Wang N, Corbett KS, Goldsmith JA, Hsieh CL, Abiona O, Graham BS, McLellan JS. Cryo-EM structure of the 2019-nCoV spike in the prefusion conformation. Science. 2020. 367(6483): 1260-1263. CrossRef
  28. Zhang X, Li Sh, Niu Sh. ACE2 and COVID-19 and the Resulting ARDS. Postgrad Med J. 2020; 96(1137): 403-407.  CrossRef
  29. Watanabe Y, Allen JD, Wrapp D, McLellan JS., Crispin M. Site-specific glycan analysis of the SARS-CoV-2 spike. Science. 2020. 369(6501): 330–333. CrossRef
  30. Hoffmann M, Kleine-Weber H, Schroeder S, Krüger N, Herrler T, Erichsen S, Schiergens TS, Herrler G, Wu NH, Nitsche A, Müller MA, Drosten C, Pöhlmann S. SARS-CoV-2 Cell Entry Depends on ACE2 and TMPRSS2 and Is Blocked by a Clinically Proven Protease Inhibitor. Cell. 2020;181(2):271-280. PubMed, PubMedCentral, CrossRef
  31. Sakai K, Ami Y, Tahara M. et al. The Host Protease TMPRSS2 Plays a Major Role in in Vivo Replication of Emerging H7N9 and Seasonal Influenza. Viruses J Virol. 2014. 88(10): 5608–5616. CrossRef
  32. Wang K, Chen W, Zhou YS. et al. SARS-CoV-2 invades host cells via a novel route: CD147-spike protein. bioRxiv. CrossRef
  33. Castelvetri LC, Ojha R, Pedro LD. et al. Neuropilin-1 facilitates SARS-CoV-2 cell entry and provides a possible pathway into the central nervous system. BioRxiv preprint. CrossRef
  34. Jia Y, Shen G, Zhang Y, Huang KS, Ho HY, Hor WS, Yang CH, Li C, Wang WL. Analysis of the mutation dynamics of SARS-CoV-2 reveals the spread history and emergence of RBD mutant with lower ACE2 binding affinity. BioRxiv. 2020. CrossRef
  35. Zhang L, Jackson CB, Mou H, Ojha A, Rangarajan ES, Izard T, Farzan M, Choe H. The D614G mutation in the SARS-CoV-2 spike protein reduces S1 shedding and increases infectivity. BioRxiv. June 12, 2020. CrossRef
  36. Thao  TTN, Labroussaa F, Ebert N, V’kovski P, Stalder H, Portmann J, Kelly J, Steiner S, Holwerda M, Kratzel A, Gultom M, Schmied K, Laloli L, Hüsser L, Wider M, Pfaender S, Hirt D, Cippà V, Crespo-Pomar S, Schröder S, Muth D, Niemeyer D, Corman VM, Müller MA, Drosten C, Dijkman R , Jores J, Thiel V. Rapid reconstruction of SARS-CoV-2 using a synthetic genomics platform. Nature. 2020;582(7813):561-565. PubMed, CrossRef
  37. Sun J, Zhuang Z, Zheng J. et al. Generation of a Broadly Useful Model for COVID-19 Pathogenesis, Vaccination, and Treatment. Cell. 2020. 182: 1–10. CrossRef
  38. Gaglia M, Lakdawala S. What we do and do not know about COVID-19’s infectious dose and viral load.
  39. Wang W, Xu Y, Gao R, Lu R, Han K, Wu G, Tan W. Detection of SARS-CoV-2 in Different Types of Clinical Specimens. JAMA. 2020. 323(18): 1843-1844. CrossRef
  40. Meyerowitz EA, Richterman AG, Quick MPH. A Summary of the COVID-19 Literature So Far. Medscape. May 18, 2020.
  41. To KK, Tsang OT, Yip CC, Chan KH, Wu TC, Chan JM, Leung WS, Chik TS, Choi CY, Kandamby DH, Lung DC, Tam AR, Poon RW, Fung AY, Hung IF, Cheng VC, Chan JF, Yuen KY. Consistent Detection of 2019 Novel Coronavirus in Saliva. Clin Infect Dis. 2020;71(15):841-843. PubMed, PubMedCentral, CrossRef
  42. Sex and Coronavirus Disease 2019 (COVID-19).
  43. Mandavilli A. Infected but Feeling Fine: The Unwitting Coronavirus Spreaders. The New York Times. July 9, 2020.
  44. Petri W. Infected with the coronavirus but not showing symptoms? A physician answers 5 questions about asymp-tomatic COVID-19. The Conversation.
  45. van Doremalen N, Bushmaker T, Morris DH, Holbrook M.G, Gamble A, Williamson BN, Tamin A, Harcourt JL, Thornburg NJ, Gerber SI, Lloyd-Smith JO, de Wit E, Munster VJ. Aerosol and Surface Stability of SARS-CoV-2 as Compared with SARS-CoV-1. N Engl J Med. 2020. 382(16): 1564-1567. CrossRef
  46. Sungnak W, Huang N, Becavin C, Berg M, Queen R, Litvinukova M, Talavera-Lopez C, Maatz H, Reichart D, Sampaziotis F, Worlock KB, Yoshida M, Barnes JL, Lung HCA. SARS-CoV-2 entry factors are highly expressed in nasal epithelial cells together with innate immune genes. Nat Med. 2020. 26: 681-687. CrossRef
  47. All Your Coronavirus Questions, Answered. Time. May 8, 2020.
  48. Lewis D. Is the coronavirus airborne? Experts can’t agree. Nature News. April 2, 2020.
  49. Harrison L. Speech May Spread More COVID-19 Than Feces. Medscape. 19 May 2020.
  50. Coronavirus: WHO rethinking how Covid-19 spreads in air. BBC News. 8 July 2020.
  51. Rapid Expert Consultation on SARS-CoV-2 Survival in Relation to Temperature and Humidity and Potential for Sea-sonality for the COVID-19 Pandemic (April 7, 2020).
  52. Recommendation Regarding the Use of Cloth Face Coverings, Especially in Areas of Significant Community-Based Transmission.
  53. Advice on the use of masks in the context of COVID-19.
  54. Estimating the number of infections and the impact of non-pharmaceutical interventions on COVID-19 in 11 European countries.
  55. Mizutani T. Signal transduction in SARS-CoV-infected cells. Ann NY Acad Sci. 2007. 1102: 86-95. CrossRef
  56. Zimmer C. DNA Inherited From Neanderthals May Increase Risk of Covid-19. The New York Times. July 4, 2020.
  57. Osterweil N. Case Fatality Rate for COVID-19 Near 1.4%, Increases With Age.
  58. Rabin RC. In Italy, Coronavirus Takes a Higher Toll on Men.
  59. Wenham C, Smith J, Morgan R. COVID-19: the gendered impacts of the outbreak. Lancet. 2020. 395(10227): 846-848. CrossRef
  60. Coronavirus in NY: Cases, maps, charts and resources.
  61. Wadman M. Sex hormones signal why virus hits men harder. Science. 2020: 368(6495): 1038-1039. CrossRef
  62. Zaiets K, Padilla R. Coronavirus, diabetes, obesity and other underlying conditions: Which patients are most at risk?
  63. Mallapaty S. Mounting clues suggest the coronavirus might trigger diabetes.
  64. Tan T, Khoo B, Mills EG. et al. Association Between High Serum Total Cortisol Concentrations and Mortality From COVID-19. Lancet Diabetes Endocrinol. 2020. 8(8): 659-660. CrossRef
  65. Sama IE, Ravera A, Santema BT. et al. Circulating Plasma Concentrations of Angiotensin-Converting Enzyme 2 in Men and Women With Heart Failure and Effects of Renin-Angiotensin-Aldosterone Inhibitors. Eur Heart J. 2020. 41(19): 1810-1817. CrossRef
  66. Murray S. New Evidence Concerning Safety of ACE Inhibitors, ARBs in COVID-19.
  67. de Simone G. Position Statement of the ESC Council on Hypertension on ACE-Inhibitors and Angiotensin Receptor Blockers. Council on Hypertension of the European Society of Cardiology. 13 Mar 2020.
  68. Radzikowska U, Ding M, Tan G, Zhakparov D, Peng Y, Wawrzyniak P, Wang M , Li S, Morita H, Altunbulakli C, Reiger M, Neumann AU, Lunjani N, Traidl-Hoffmann C, Nadeau KC, O’Mahony L, Akdis C, Sokolowska M. Distribution of ACE2, CD147, CD26, and other SARS-CoV-2 associated molecules in tissues and immune cells in health and in asthma, COPD, obesity, hypertension, and COVID-19 risk factors. Allergy. 2020;75(11):2829-2845.
    PubMed, PubMedCentral, CrossRef
  69. Report of the WHO-China Joint Mission on Coronavirus Disease 2019 (COVID-19).
  70. Liu R, Wang Y, Li J, Han H, Xia Z, Liu F, Wu K, Yang L, Liu X, Zhu C. Decreased T cell populations contribute to the increased severity of COVID-19. Clin Chim Acta. 2020;508:110-114. PubMed, PubMedCentral, CrossRef
  71. Ying T, Li W, Dimitrov DS. Discovery of T-Cell Infection and Apoptosis by Middle East Respiratory Syndrome Coro-navirus. J Infect Dis. 2016. 213(6): 877-879. CrossRef
  72. Shoenfeld Y. Corona (COVID-19) time musings: Our involvement in COVID-19 pathogenesis, diagnosis, treatment and vaccine planning. Autoimmun Rev. 2020. 19(6): 102538. CrossRef
  73. Cascella M, Rajnik M, Cuomo A, Dulebohn SC, Di Napoli R. Features, Evaluation and Treatment Coronavirus (COVID-19). (Treasure Island (FL): Stat Pearls Publishing, 2020).
  74. Long B, Brady WJ, Koyfman A, Gottlieb M. Cardiovascular complications in COVID-19. Am  J Emerg Med. 2020. CrossRef
  75. Xu L, Liu J, Lu M, Yang D, Zheng X. Liver injury during highly pathogenic human coronavirus infections. Liver International. 2020. 40(5): 998-1004.  CrossRef
  76. Riphagen S, Gomez X, Gonzalez-Martinez C, Wilkinson N, Theocharis P. Hyperinflammatory shock in children during COVID-19 pandemic. Lancet. 2020;395(10237):1607-1608. PubMed, PubMedCentral, CrossRef
  77. Kwon D. From Headaches to ‘COVID Toes,’ Coronavirus Symptoms Are a Bizarre Mix. Blood clots and inflammation may underlie many of these complications.
  78. Carod-Artal F.J. Neurological complications of coronavirus and COVID-19. Revista de Neurologia. 2020. 70(9): 311-322. CrossRef
  79. Brooks M. COVID-19 Tied to Wide Range of Neuropsychiatric Complications. Medscape. June 29, 2020.
  80. Cormier Z. How Covid-19 can damage the brain. BBC News. 23 June 2020.
  81. Yasgur BS. Three Stages to COVID-19 Brain Damage, New Review Suggests. Medscape. June 29, 2020.
  82. Negro F. Is antibody-dependent enhancement playing a role in COVID-19 pathogenesis? Swiss Med. Weekly. 2020. 150: w20249.  CrossRef
  83. Coronavirus disease (COVID-19) technical guidance: Laboratory testing for 2019-nCoV in humans.
  84. Summary table of available protocols in this document.
  85. SARS-CoV-2 Diagnostic Pipeline.
  86. Coronavirus Testing Picks Up in the U.S. Following Slow Start.
  87. Liuqian L, Shulun H, Wei H. 14% of Recovered Covid-19 Patients in Guangdong Tested Positive Again.
  88. Omer SB, Malani P, Del Rio C. The COVID-19 Pandemic in the US: A Clinical Update. JAMA. 2020;323(18):1767-1768. PubMed, CrossRef
  89. Parry RL. Coronavirus patients can’t relapse, South Korean scientists believe.
  90. Ukraine has developed test systems to detect antibodies to coronavirus. The Day. April, 13, 2020. (In Ukrainian).
  91. Sona Nanotech Inc. Buy.
  92. Sheridan C. Fast, portable tests come online to curb coronavirus pandemic.
  93.  Fletcher ER, Vijay SL. New COVID-19 Rapid Diagnostic Approved On ‘GeneXpert’ TB Platform; Could Pave Way For More Testing In Low- & Middle-Income Countries.
  94. Coronavirus (COVID-19) Update: FDA Authorizes First Antigen Test to Help in the Rapid Detection of the Virus that Causes COVID-19 in Patients.
  95. Sofia 2 SARS antigen FIA.

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