Ukr.Biochem.J. 2021; Volume 93, Issue 1, Jan-Feb, pp. 104-112

doi: https://doi.org/10.15407/ubj93.01.104

Application of fluorescein copolymer to improve the efficiency of counter-immunoelectrophoresis for diagnostics of animal infectious diseases

D. D. Ostapiv1, N. V. Kuz’mina1, М. R. Kozak1, Shan Hu2,
V. V. Vlizlo3*, I. Ya. Kotsiumbas3, S. M. Varvarenko4,
V. Ya. Samaryk4, N. G. Nosova4, M. V. Yakoviv4

1Institute of Animal Biology, NAAS of Ukraine, Lviv, Ukraine;
2Qingdao Agricultural University, Qingdao, China;
3State Scientific-Research Control Institute of Veterinary Medicinal Products and Feed Additives, Lviv, Ukraine;
4Lviv Polytechnic National University, Lviv, Ukraine;
*e-mail: vasyl.vlizlo@scivp.lviv.ua

Received: 12 May 2020; Accepted: 17 December 2020

The counter-immunoelectrophoresis (CIE) is a highly specific, simple and rapid method, which does not require expensive equipment, input materials and long-term staff training as compared with other express methods of viral infections diagnostic. Despite the CIE high informativeness, the results in practice can be doubtful at times. Therefore, the purpose of the study was to improve CIE method by strengthening the antigen-antibody interaction with the use of synthesized amphiphilic fluorescein-containing copolymer. It was demonstrated that the use of the fluorescein copolymer -antigen complex enhanced the interaction with serum/plasma antibodies of rabbits immunized against hemorrhagic disease and  increased  protein content in the area of precipitation. This effect was more pronounced when more hydrophobic copolymer containing 5.87% fluorescein was introduced into the complex with antigen. The ability of the amphiphilic fluorescein copolymer to enhance the antigen-antibody interaction and the ability to visualize the fluorescent precipitation zone may increase CIE effectiveness for express diagnostic of  infectious diseases.

Keywords: , , ,


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