Ukr.Biochem.J. 2023; Volume 95, Issue 2, Mar-Apr, pp. 83-92

doi: https://doi.org/10.15407/ubj95.02.083

Characterization of lectins from wheat seedlings infected with Fusarium graminearum and treated by jasmonic acid

13.06.2023   Uncategorized   No comments

O. О. Molodchenkova1*, O. V. Ryshchakova1,
T. V. Kartuzova1, L. T. Mishchenko2

1Plant Breeding and Genetics Institute – National Center of Seed
and Cultivar Investigation, Laboratory of Plant Biochemistry, Odesa, Ukraine;
*e-mail: olgamolod@ukr.net;
2ESC “Institute of Biology and Medicine”,
Taras Shevchenko National University of Kyiv, Ukraine;
e-mail: lmishchenko@ukr.net

Received: 24 February 2023; Revised: 27 April 2023;
Accepted: 05 June 2023; Available on-line: 20 June 2023

Fusarium head blight is one of the most serious diseases of wheat caused by a range of Fusarium fungi, which infects the heads of the crop, reducing grain yield. Lectins that specifically bind carbohydrate ligands of various chemical nature and Jasmonic acid (JA) as a key regulator of plant development play an important role in plant protective responses to biotic factors. The goal of the study was to determine the activity and biochemical characteristics of soluble lectins in wheat seedlings of varieties ‘Lastivka odeska’ with a high resiliency to Fusarium graminearum and ‘Nikonia odeska’ susceptible to Fusarium graminearum­. Wheat seedlings were grown on the media containing pathogenic infection or JA solution. Lectins were purified by affinity chromatography and separated by electrophoresis in 15% PAGE. Lectin activity was determined by the method of trypsinized blood erythrocytes hemagglutination. Molecular mass of the main components of lectins from ‘Lastivka odeska’ seedlings was determined to be 67, 60, 45 kDa, and of the main component of lectins from ‘Nikonia odeska’ seedlings – 45 kDa. Lectins isolated from the control untreated seedlings had preferential affinity for N-acetylglucosamine, D-galactosamine and D-fructose-6-phosphate. It was shown that both at pathogen action or JA treatment, lectin activity in the seedlings of resistant variety ‘Lastivka odeska’ was increased while in the seedlings of susceptible variety ‘Nikonia odeska’ it was decreased as compared to control. At the joint action of pathogen and JA, lectin activity in the seedlings of susceptible variety increased compared with the infected seedlings. The results obtained can be used for the development of biochemical methods for assessing the degree of wheat varieties resiliency to fusariose.

Keywords: , , , , ,

References:

  1. El Chami J, El Chami E, Tarnawa Á, Kassai KM, Kende Z, Jolánkai M. Effect of Fusarium infection on wheat quality parameters. Cereal Res Commun. 2023;51(1):179-187. CrossRef
  2. Zhu Z, Hao Y, Mergoum M, Bai G, Humphreys G, Cloutier S, Xia X, He Z. Breeding wheat for resistance to Fusarium head blight in the Global North: China, USA, and Canada. Crop J. 2019;7(6):730–738. CrossRef
  3. Jain S, Kumar A. The pathogenesis related class 10 proteins in plant defense against biotic and abiotic stresses. Adv Plants Agric Res. 2015;2(7):305-314. CrossRef
  4. Santos AFS, da Silva MDC, Napoleão TH, Paiva PMG, Correia MTS, Coelho LCB. B. Lectins: Function, structure, biological properties and potential applications. Curr Top Pept Protein Res. 2014;15:41-62.
  5. Oliveira MV, Osterne VJS, Lossio CF, Serna S, Reichardt NC, Nascimento KS, Van Damme EJM, Cavada BS. Purification and characterization of a highly thermostable GlcNAc-binding lectin from Collaea speciosa seeds. Int J Biol Macromol. 2021;193(Pt B):1562-1571. PubMed, CrossRef
  6. Itakura Y, Nakamura-Tsuruta S, Kominami J, Tateno H, Hirabayashi J. Sugar-Binding Profiles of Chitin-Binding Lectins from the Hevein Family: A Comprehensive Study. Int J Mol Sci. 2017;18(6):1160. PubMed, PubMedCentral, CrossRef
  7. Naithani S, Komath SS, Nonomura A, Govindjee G. Plant lectins and their many roles: Carbohydrate-binding and beyond. J Plant Physiol. 2021;266:153531. PubMed, CrossRef
  8. Breitenbach Barroso Coelho LC, Marcelino Dos Santos Silva P, Felix de Oliveira W, de Moura MC, Viana Pontual E, Soares Gomes F, Guedes Paiva PM, Napoleão TH, Dos Santos Correia MT. Lectins as antimicrobial agents. J Appl Microbiol. 2018;125(5):1238-1252. PubMed, CrossRef
  9. Van Damme EJM. 35 years in plant lectin research: a journey from basic science to applications in agriculture and medicine. Glycoconj J. 2022;39(1):83-97. PubMed, PubMedCentral, CrossRef
  10. Lannoo N, Van Damme EJM. Lectin domains at the frontiers of plant defense. Front Plant Sci. 2014;5:397. PubMed, PubMedCentral, CrossRef
  11. Ma QH, Tian B, Li YL. Overexpression of a wheat jasmonate-regulated lectin increases pathogen resistance. Biochimie. 2010;92(2):187-193. PubMed, PubMedCentral, CrossRef
  12. Babayants OV, Babayants LT. Bases of breeding and methodology of assessments of wheat resistance to pathogens. Odessa: VМV, 2014. 401 p. (in Russian).
  13. Molodchenkova OO, Adamovska VG. Wheat defense reactions at the action of Fusarium graminearum, salicylic and jasmonic acids. Plant Physiol Genet. 2015; 47(5):447-458. (In Ukrainian).
  14.  Lutsik MD, Panasyuk EN, Lutsik AD. Lectins. Lviv: Vishcha school, 1981. 155 p. (In Russian).
  15. Lowry OH, Rosebrough NJ, Farr AL, Randall RJ. Protein measurement with the Folin phenol reagent. J Biol Chem. 1951;193(1):265-275. PubMed
  16. Osterman LA. Methods for the study of proteins and nucleic acids: Electrophoresis and ultracentrifugation. M.: Nauka, 1981. 288 p. (In Russian).
  17. Моlоdchenkova OO, Adamovskaya VG, Tsiselskaya LY, Sagaydak TV. Isolation and properties of lectins from cell walls of wheat seedlings infected by Fusarium graminearum and treated by salicylic acid. Ukr Biokhim Zhurn. 2010;82(5):111-117. (In Russian). PubMed
  18. Моlodchenkova ОО, Ryshchakova OV. Participation of lectins in the formation of plant protective reaction of cereals. New stages of development of modern science in Ukraine and EU countries: Collective monograph. Riga: Baltija Publishing”, 2019. P. 205-230. CrossRef
  19. Tsaneva M, Van Damme EJM. 130 years of Plant Lectin Research. Glycoconj J. 2020;37(5):533-551. PubMed, PubMedCentral, CrossRef
  20. Schutter KD, Van Damme EJM. Protein-carbohydrate interactions as part of plant defense and animal immunity. Molecules. 2015;20(5):9029-9053. PubMed, PubMedCentral, CrossRef
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