Ukr.Biochem.J. 2024; Volume 96, Issue 3, May-Jun, pp. 97-107


Internal lipids and their fatty acids composition in a sheep wool fiber under biodestruction with fleece microorganisms

V. M. Tkachuk1*, P. V. Stapay2, N. Z. Ohorodnyk1, N. R. Motko3

1Lviv National Environmental University, Dubliany, Lviv Region, Ukraine;
2Institute of Animal Biology, National Academy of Agrarian Sciences, Lviv, Ukraine;
3Stepan Gzhytskyi National University of Veterinary Medicine
and Biotechnologies of Lviv, Ukraine;

Received: 18 January 2024; Revised: 23 February 2024;
Accepted: 31 May 2024; Available on-line: 17 June 2024

Microbiological destruction of fibers is a common damage to sheep’s wool. Considering the defining role of internal lipids in the formation of wool fibers surface the aim of the work was to study the structure and lipid composition of the normal and damaged wool. The research was carried out on ewes of the Askanian fine-wool breed. The content of microorganisms was estimated after sowing on dense nutrient environments. Wool fibers surface was studied by scanning electron microscopy, the content of internal lipids by thin layer chromatography after preliminary alkaline hydrolysis of the fiber, and fatty acids composition by gas-liquid chromatography. Biodestructed wool was shown to contain almost three times more bacteria, as well as higher levels of actinomycetes and mushrooms compared to intact wool. The violation of the cuticular layer was detected as the result of the fleece microflora activity. In a defective wool the content of the free internal lipids and non-esterified fatty acids was increased while the content of protein-bound lipids and esterified cholesterol as well as of ceramides was decreased as compared to normal wool. The level of 18-methyleicosanoic acid in the protein-bound lipids of damaged wool was decreased, indicating the destruction of the thioester bonds by which structural lipids are covalently linked to proteins through 18-methyleicosanoic acid.

Keywords: , , , , , ,


  1. Ghimis SB, Mirt A.L, Vlaicu A, Zaharia E, Bomboş MM, Vasilievici G. Impregnated sheep wool fibers with an antimicrobial effect. Chem Proc. 2023;13(1):1. CrossRef
  2. Sanders D, Grunden A, Dunn RR. A review of clothing microbiology: the history of clothing and the role of microbes in textiles. Biol Lett. 2021;17(1):20200700. PubMed, PubMedCentral, CrossRef
  3. Jackson TA, Pearson JF, Young SD, Armstrong J, O’Callaghan M. Abundance and distribution of microbial populations in sheep fleece. New Zealand J Agric Res. 2002;45(1):49-55. CrossRef
  4. Colditz I, Vuocolo Т, Denman S, Ingham A, Wijffels G, James P, Tellam R. Fleece rot in sheep: a review of pathogenesis, aetiology, resistance and vaccines. Anim Prod Sci. 2022;62(3):201-215. CrossRef
  5. Queiroga AC, Pintado ME, Malcata FX. Wool-associated proteolytic bacteria, isolated from Portuguese Merino breed. Small Rumin Res. 2013;109(1):38-46. CrossRef
  6. Caven B, Redl B, Bechtold T. An investigation into the possible antibacterial properties of wool fibers. Textile Res J. 2019;89(4):510-516. CrossRef
  7. Denman S, Tellam R, Vuocolo T, Ingham A, Wijffels G, James P, Colditz I. Fleece rot and dermatophilosis (lumpy wool) in sheep: opportunities and challenges for new vaccines. Anim Prod Sci. 2022;62(4):301-320. CrossRef
  8. Harmsen P. Biological degradation of textiles: and the relevance to textile recycling. Netherlands: Wageningen Food & Biobased Research, 2021. 21 p. CrossRef
  9. Kavkler K, Demšar A. Impact of fungi on contemporary and accelerated aged wool fibres. Polym Degrad Stabil. 2012;97(5):786-792. CrossRef
  10. Stapay PV, Tkachuk VM. Yellowing of sheep’s wool. Lviv: ZUKC, 2011. 96 р. (In Ukrainian).
  11. Albanell CB, Carrer V, Marti M, Iglesias J, Iglesias J, Coderch L. Solvent-Extracted Wool Wax: Thermotropic Properties and Skin Efficacy. Skin Pharmacol Physiol. 2018;31(4):198-205. PubMed, CrossRef
  12. Seiko J, Sabu T, Gautam B. The wool handbook: morphology, structure, properties, processing, and applications. Woodhead Publishing, Oxford, 2023. 450 p.
  13. Ghermezgoli ZM, Moghaddam MK, Moezzi M. Chemical, morphological and structural characteristics of crossbred wool fibers. J Textile Institute. 2020; 111(5): 709-717. CrossRef
  14. Csuka DA, Csuka EA, Juhász MLW, Sharma AN., Mesinkovska NA. A systematic review on the lipid composition of human hair. Int J Dermatol. 2023;62(3):404-415. PubMed, CrossRef
  15. Coderch L, Oliver MA, Martínez V, Manich AM, Rubio L, Martí M. Exogenous and endogenous lipids of human hair. Skin Res Technol. 2017;23(4):479-485. PubMed, CrossRef
  16. Song SH, Lim JH, Son SK, Choi J, Kang NG, Lee SM. Prevention of lipid loss from hair by surface and internal modification. Sci Rep. 2019;9(1):9834. PubMed, PubMedCentral, CrossRef
  17. Coderch L, Alonso C, García MT, Pérez L, Martí M. Hair lipid structure: effect of surfactants. Cosmetics. 2023;10(4):107. CrossRef
  18. Tokunaga S, Tanamachi H, Ishikawa K. Degradation of Hair Surface: Importance of 18-MEA and Epicuticle. Cosmetics. 2019;6(2):31. CrossRef
  19. Tkachuk VM, Havrylyak VV, Stapay PV, Sedilo HM. Internal lipids of felted, yellowed and pathologically thin wool. Ukr Biochem J. 2014;86(1):131-138. PubMed, CrossRef
  20. Coderch L, Oliver MA, Carrer V, Manich AM, Martí M. External lipid function in ethnic hairs. J Cosmet Dermatol. 2019;18(6):1912-1920. PubMed, CrossRef
  21. Das S, Dash HR. Microbial biotechnology – a laboratory manual for bacterial systems. India, New Delhi: Springer, 2015. 239 p. CrossRef
  22. Fedorenko VO, Ostash BO, Honchar MV, Rebetz YV. Great practical manual on genetics, genetic engineering, and analytical biotechnology of microorganisms. Lviv: Publishing Center of Ivan Franko National University, 2007. 279 p. (In Ukrainian).
  23. Tkachuk VM, Stapay PV. Investigation of grease-sweat wax and wool lipids: Methodical recommendations. Lviv, 2011. 24 p. (In Ukrainian).
  24. Wertz PW, Downing TD. Integral lipids of human hair. Lipids. 1988;23(9):878-881. PubMed, CrossRef
  25. Marsh JB, Weinstein DB. Simple charring method for determination of lipids. J Lipid Res. 1966;7(4):574-576. PubMed
  26. Wertz PW, Downing TD. Integral lipids of mammalian hair. Comp Biochem Physiol B. 1989;92(4):759-761. PubMed, CrossRef
  27. Stoffel W, Chu F, Ahrens EH. Analysis of long-chain fatty acids by acids by gas-iquid chromatography. Anal Chem. 1959;31(2):307-308. CrossRef
  28. Stapay PV, Tkachuk VM, Sedilo GM, Ogorodnyk NZ. Lipids of sheep skin and wool, their role in wool formation and preservation of natural fiber properties. Lviv: Bona, 2019. 332 p. (In Ukrainian).
  29. Chandra P, Enespa, Singh R, Arora PK. Microbial lipases and their industrial applications: a comprehensive review. Microb Cell Fact. 2020;19(1):169. PubMed, PubMedCentral, CrossRef
  30. Rogers GE. Known and unknown features of hair cuticle structure: A brief review. Cosmetics. 2019;6(2):32. CrossRef
  31. Marsh JM, Whitaker S, Felts T, Shearouse W, Vatter M, Määttä A, Thompson M, Hawkins TJ. Role of Internal Lipids in Hair Health. J Cosmet Sci. 2018;69(5):347-356. PubMed
  32. Stapay P, Stakhiv N, Smolianinova O, Grabovska O, Tyutyunnyk O. Sulfur-containing compounds of wool and their role in the processes of wool growth and the formation of physicochemical properties of fibers. Sci Works NUFT. 2021; 27 (5): 21-32. (In Ukrainian). CrossRef
  33. Fandrei F, Engberg O, Opálka L, Jančálková P, Pullmannová P, Steinhart M, Kováčik A, Vávrová K, Huster D. Cholesterol sulfate fluidizes the sterol fraction of the stratum corneum lipid phase and increases its permeability. J Lipid Res. 2022;63(3):100177. PubMed, PubMedCentral, CrossRef
  34. Smith JR, Swift JA. Lamellar subcomponents of the cuticular cell membrane complex of mammalian keratin fibres show friction and hardness contrast by AFM. J Microsc. 2002;206(Pt 3):182-193. PubMed, CrossRef
  35. Wang E, Klauda JB. Simulations of Pure Ceramide and Ternary Lipid Mixtures as Simple Interior Stratum Corneum Models. J Phys Chem B. 2018;122(10):2757-2768. PubMed, CrossRef
  36. Havryliak VV, Tkachuk VM. Fatty acid composition of structural lipids of normal and abnormal wool fibres. Ukr Biokhim Zhurn. 2012;84(5):106-111. (In Ukrainian). PubMed
  37. Sanders JM, Coscia BJ, Fonari A, Misra M, Mileo PGM, Giesen DJ, Browning AR, Halls MD. Exploring the Effects of Wetting and Free Fatty Acid Deposition on an Atomistic Hair Fiber Surface Model Incorporating Keratin-Associated Protein 5-1. Langmuir. 2023;39(15):5263-5274. PubMed, CrossRef
  38. Stapay PV, Stakhiv NP, Tkachuk VM, Smolianinova OO. The relationship between structural lipids of sheep wool with its individual macrostructural components, chemical composition and physical indicators. Bìol Tvarin. 2021;23(1): 38-43. (In Ukrainian). CrossRef

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