Ukr.Biochem.J. 2024; Volume 96, Issue 5, Sep-Oct, pp. 96-103

doi: https://doi.org/10.15407/ubj96.05.096

Short-term reduction in feed intake by dairy cows in the postpartum period leads to subclinical ketosis development

17.10.2024   Uncategorized   No comments

V. Vlizlo1,2*, O. Stasiv1, H. Sedilo1,
N. Fedak1, M. Petryshyn1, R. Voloshyn1

1Institute of Agriculture of Carpathian Region the National Academy
of Agrarian Sciences of Ukraine, Obroshyne Lviv district of Lviv region, Ukraine;
2Stepan Gzhytskyi National University of Veterinary Medicine
and Biotechnologies Lviv, Lviv, Ukraine;
*e-mail: vasyl.vlizlo@lvet.edu.ua

Received: 22 May 2024; Revised: 08 August 2024;
Accepted: 07 October 2024; Available on-line: 28 October 2024

Subclinical ketosis is widespread in highly productive dairy cows after calving and often remains undiagnosed, leading to reduced productivity. Physiologically controlled feeding in the first weeks after calving and during the intensive lactation period can reduce the incidence of ketosis. The study aimed to determine how a short-term reduction of feed after calving affects the formation of ketone bodies in blood, urine and milk of dairy cows. The group of ten Ukrainian black-spotted dairy breed cows after calving aged from 4 to 6 years was involved in the experiment lasted for 72 h. In 24 h the amount of compound feed, haylage, and silage received by cows was gradually reduced until complete exclusion in the diet. Blood, urine, and milk samples were collected three times a day. The content of ketone bodies, β-hydroxybutyrate and glucose was estimated with the corresponding sets of indicator strips. The content of total bilirubin, cholesterol, albumin and enzymes activity in the blood serum were determined on biochemical analyzer. It was found that in 24 h after the beginning of feed reduction the level of blood glucose decreased, persistent hypoglycemia within 48 and 72 h was developed, the concentration of β-hydroxybutyrate in blood and milk and of ketone bodies in urine was elevated. The increase in total bilirubin concentration and liver enzymes activity in the blood serum with a simultaneous decrease in albumin level and delayed sodium propionate conversion into glucose were observed. Thus, short-term reduction in feed intake by dairy cows after calving causes ketosis development and violation of liver functions.

Keywords: , , , , ,

References:

  1. Raja S, Palanisamy M, Prabaharan V, Rajkumar R, Kumar SS. Prevalence of subclinical ketosis in anestrum dairy cows. J Entomol Zool Stud. 2021;9(1):2044-2046.
  2. Stivanin SCB, Vizzotto EF, Matiello JP, Machado FS, Campos MM, Tomich ThR, Pereira LGR, Fischer V. Behavior, feed intake and health status in Holstein, Gyr and Girolando-F1 cows during the transition period: Behavior and health of dairy cows in the transition period. Appl Anim Behav Sci. 2021;242:105403. CrossRef
  3. González FD, Muiño R, Pereira V, Campos R, Benedito JL. Relationship among blood indicators of lipomobilization and hepatic function during early lactation in high-yielding dairy cows. J Vet Sci. 2011;12(3):251-255. PubMed, PubMedCentral, CrossRef
  4. Xu W, Vervoort J, Saccenti E, Kemp B, van Hoeij RJ, van Knegsel ATM. Relationship between energy balance and metabolic profiles in plasma and milk of dairy cows in early lactation. J Dairy Sci. 2020;103(5):4795-4805. PubMed, CrossRef
  5. Li S, Wei X, Song J, Zhang C, Zhang Y, Sun Y. Evaluation of Statistical Process Control Techniques in Monitoring Weekly Body Condition Scores as an Early Warning System for Predicting Subclinical Ketosis in Dry Cows. Animals (Basel). 2021;11(11):3224. PubMed, PubMedCentral, CrossRef
  6. Ha S, Kang S, Jeong M, Han M, Lee J, Chung H, Park J. Characteristics of Holstein cows predisposed to ketosis during the post-partum transition period. Vet Med Sci. 2023;9(1):307-314. PubMed, PubMedCentral, CrossRef
  7. Atalay H. The effect of body condition score on nutritional diseases and milk yield in dairy cattle. Turk J Vet Anim Sci. 2019;43(5):692-697. CrossRef
  8. Schulz K, Frahm J, Meyer U, Kersten S, Reiche D, Rehage J, Dänicke S. Effects of prepartal body condition score and peripartal energy supply of dairy cows on postpartal lipolysis, energy balance and ketogenesis: an animal model to investigate subclinical ketosis. J Dairy Res. 2014;81(3):257-266. PubMed, CrossRef
  9. Gruber S, Mansfeld R. Herd health monitoring in dairy farms – discover metabolic diseases. An overview. Tierarztl Prax Ausg G Grosstiere Nutztiere. 2019;47(4):246-255. PubMed, CrossRef
  10. Seifi HA, Huzzey JM, Khan MA, Weary DM, von Keyserlingk MAG. Addition of straw to the early-lactation diet: Effects on feed intake, milk yield, and subclinical ketosis in Holstein cows. J Dairy Sci. 2021;104(3):3008-3017. PubMed, CrossRef
  11. Smith GL, Friggens NC, Ashworth CJ, Chagunda MGG. Association between body energy content in the dry period and post-calving production disease status in dairy cattle. Animal. 2017;11(9):1590-1598. PubMed, PubMedCentral, CrossRef
  12. Vudmaska I, Petrukh I, Sachko S, Vlizlo V, Kosenko Y, Kozak M, Petruk A. Using hop cones, vitamin E, methionine, choline and carnitine for treatment of subclinical ketosis in transition dairy cows. Adv Anim Vet Sci. 2021;9(1):55-62. CrossRef
  13. Suthar VS, Canelas-Raposo J, Deniz A, Heuwieser W. Prevalence of subclinical ketosis and relationships with postpartum diseases in European dairy cows. J Dairy Sci. 2013;96(5):2925-2938. PubMed, CrossRef
  14. Brunner N, Groeger S, Canelas Raposo J, Bruckmaier RM, Gross JJ. Prevalence of subclinical ketosis and production diseases in dairy cows in Central and South America, Africa, Asia, Australia, New Zealand, and Eastern Europe. Transl Anim Sci. 2018;3(1):84-92. PubMed, PubMedCentral, CrossRef
  15. Loiklung C, Sukon P, Thamrongyoswittayakul C. Global prevalence of subclinical ketosis in dairy cows: A systematic review and meta-analysis. Res Vet Sci. 2022;144:66-76. PubMed, CrossRef
  16. Steeneveld W, Amuta P, van Soest FJS, Jorritsma R, Hogeveen H. Estimating the combined costs of clinical and subclinical ketosis in dairy cows. PLoS One. 2020;15(4):e0230448. PubMed, PubMedCentral, CrossRef
  17. Rodriguez Z, Shepley E, Endres MI, Cramer G, Caixeta LS. Assessment of milk yield and composition, early reproductive performance, and herd removal in multiparous dairy cattle based on the week of diagnosis of hyperketonemia in early lactation. J Dairy Sci. 2022;105(5):4410-4420. PubMed, CrossRef
  18. Alemu TW, Santschi DE, Cue RI, Duggavathi R. Reproductive performance of lactating dairy cows with elevated milk β-hydroxybutyrate levels during first 6 weeks of lactation. J Dairy Sci. 2023l;106(7):5165-5181. PubMed, CrossRef
  19. Gröhn Y. Propionate loading test for liver function in spontaneously ketotic dairy cows. Res Vet Sci. 1985;39(1):24-28. PubMed, CrossRef
  20. Ruoff J, Borchardt S, Heuwieser W. Short communication: Associations between blood glucose concentration, onset of hyperketonemia, and milk production in early lactation dairy cows. J Dairy Sci. 2017;100(7):5462-5467. PubMed, CrossRef
  21. Sabek A, Li C, Du C, Nan L, Ni J, Elgazzar E, Ma Y, Salem AZM, Zhang S. Effects of parity and days in milk on milk composition in correlation with β-hydroxybutyrate in tropic dairy cows. Trop Anim Health Prod. 2021;53(2):270. PubMed, CrossRef
  22. Mezzetti M, Minuti A, Piccioli-Cappelli F, Amadori M, Bionaz M, Trevisi E. The role of altered immune function during the dry period in promoting the development of subclinical ketosis in early lactation. J Dairy Sci. 2019;102(10):9241-9258. PubMed, CrossRef
  23. Habel J, Sundrum A. Mismatch of Glucose Allocation between Different Life Functions in the Transition Period of Dairy Cows. Animals (Basel). 2020;10(6):1028. PubMed, PubMedCentral, CrossRef
  24. Vanholder T, Papen J, Bemers R, Vertenten G, Berge AC. Risk factors for subclinical and clinical ketosis and association with production parameters in dairy cows in the Netherlands. J Dairy Sci. 2015;98(2):880-888. PubMed, CrossRef
  25. Snedec T, Theinert K, Pietsch F, Leonhardt AS, Kadáši M, Klein R, Spilke J, Bannert E, Schären M, Köller G, Baumgartner W, Theile S, Kaiser A, Rachidi F, Starke A. Validation of a cow-side blood test for beta-hydroxybutyrate concentration in dairy cows. Tierarztl Prax Ausg G Grosstiere Nutztiere. 2021;49(1):5-11. PubMed, CrossRef
  26. McArt JAA, Nydam DV, Oetzel GR. Epidemiology of subclinical ketosis in early lactation dairy cattle. J Dairy Sci. 2012;95(9):5056-5066. PubMed, CrossRef
  27. Du X, Zhu Y, Peng Z, Cui Y, Zhang Q, Shi Z, Guan Y, Sha X, Shen T, Yang Y, Li X, Wang Z, Li X, Liu G. High concentrations of fatty acids and β-hydroxybutyrate impair the growth hormone-mediated hepatic JAK2-STAT5 pathway in clinically ketotic cows. J Dairy Sci. 2018;101(4):3476-3487. PubMed, CrossRef
  28. Benedet A, Manuelian CL, Zidi A, Penasa M, De Marchi M. Invited review: β-hydroxybutyrate concentration in blood and milk and its associations with cow performance. Animal. 2019;13(8):1676-1689. PubMed, CrossRef
  29. Hubner A, Canisso IF, Peixoto PM, Coelho WM Jr, Ribeiro L, Aldridge BM, Menta P, Machado VS, Lima FS. Characterization of metabolic profile, health, milk production, and reproductive outcomes of dairy cows diagnosed with concurrent hyperketonemia and hypoglycemia. J Dairy Sci. 2022;105(11):9054-9069. PubMed, CrossRef
  30. Antanaitis R, Džermeikaitė K, Januškevičius V, Šimonytė I, Baumgartner W. In-Line Registered Milk Fat-to-Protein Ratio for the Assessment of Metabolic Status in Dairy Cows. Animals (Basel). 2023;13(20):3293. PubMed, PubMedCentral, CrossRef
  31. Couperus AM, Schroeder F, Hettegger P, Huber J, Wittek T, Peham JR. Longitudinal Metabolic Biomarker Profile of Hyperketonemic Cows from Dry-Off to Peak Lactation and Identification of Prognostic Classifiers. Animals (Basel). 2021;11(5):1353.  PubMed, PubMedCentral, CrossRef
  32. Vlizlo V, Prystupa O, Slivinska L, Gutyj B, Maksymovych I, Shcherbatyy A, Lychuk M, Partyka U, Chernushkin B, Rusyn V, Leno M, Leskiv K. Treatment of animals with fatty liver disease using a drug based on the seeds of Silybum marianum. Regul Mech Biosyst. 2023;14(3):424-431. CrossRef
  33. Ha S, Kang S, Han M, Lee J, Chung H, Oh SI, Kim S, Park J. Predicting ketosis during the transition period in Holstein Friesian cows using hematological and serum biochemical parameters on the calving date. Sci Rep. 2022;12(1):853. PubMed, PubMedCentral, CrossRef
  34. Rodriguez Z, Picasso-Risso C, Gaire TN, Nakagawa K, Noyes N, Cramer G, Caixeta L. Evaluating variations in metabolic profiles during the dry period related to the time of hyperketonemia onset in dairy cows. PLoS One. 2023;18(8):e0289165. PubMed, PubMedCentral, CrossRef
  35. Simonov M, Vlizlo V. Some blood markers of the functional state of liver in dairy cows with clinical ketosis. Bulgarian J Vet Med. 2015;18(1):74-82.
    CrossRef
  36. Tóthová C, Nagy O, Nagyová V, Kováč G. Serum protein electrophoretic pattern in dairy cows during the periparturient period. J Appl Anim Res. 2016;46(1):33-38. CrossRef
  37. Đoković R, Šamanc H, Petrović MD, Ilić Z, Kurćubić V. Relationship among blood metabolites and lipid content in the liver in transitional dairy cows. Biotechnol Anim Husbandry. 2012;28(4):705-714. CrossRef
  38. Rodriguez-Jimenez S, Haerr KJ, Trevisi E, Loor JJ, Cardoso FC, Osorio JS. Prepartal standing behavior as a parameter for early detection of postpartal subclinical ketosis associated with inflammation and liver function biomarkers in peripartal dairy cows. J Dairy Sci. 2018;101(9):8224-8235. PubMed, CrossRef
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