Ukr.Biochem.J. 2025; Volume 97, Issue 3, May-Jun, pp. 96-107

doi: https://doi.org/10.15407/ubj97.03.096

Influence of heterometallic Ge(IV) – 3d-metals complexes on microbial rhamnosidase, galactosidase and protease activity

25.06.2025   Uncategorized   No comments

O. V. Gudzenko1*, L. D. Varbanets1, I. I. Seifullina2,
О. E. Martsynko2, O. A. Finik3, K. K. Tsymbaliuk2,3

1Zabolotny Institute of Microbiology and Virology,
National Academy of Sciences of Ukraine, Kyiv;
2Odesa I. I. Mechnikov National University, Ukraine;
3LLC “Inspectorat Ukraine”, Ukraine;
*e-mail: alena.gudzenko81@gmail.com

Received: 18 March 2025; Revised: 12 May 2025;
Accepted: 11 June 2025; Available on-line: 07 July 2025

In recent years, the attention of researchers has been attracted by coordination compounds of germanium with various bioligands, which may be used both as activator or inhibitor of enzymes. The aim of the work was to investigate the effect of new heterometallic Ge(IV) – 3d-metals complexes with 1-hydro­xyethane-1,1-diphosphonic acid and 1,10-phenanthroline on the activity of purified α-L-rhamnosidases produced by Eupenicillium erubescens, Penicillium tardum, Penicillium restrictum, Cryptococcus albidus, α-galactosidase of P. restrictum and proteases with elastolytic and fibrinogenolytic activity of Bacillus sp. The studied compounds (0,1% concentration) activate α-L-rhamnosidase differently depending on its producer. Thus, the activity of α-L-rhamnosidase of E. erubescens was stimulated with [Co(phen)3]4[Ge6(μ-OH)4(μ-O)2(μ-hedp)6]·2СН3СООН·30H2O by 200%, of Penicillium tardum – with [Zn(phen)2(H2O)2]2[Zn(phen)(H2O)4]2[Ge6(μ-OH)4(μ-O)2(μ-hedp)6]·18H2O by 200%, of Penicillium restrictum – with [Ni(phen)3]4[Ge6(μ-OH)4(μ-O)2(μ-hedp)6]·2СН3СООН·26H2O by 67%, of Cryptococcus albidus – wih [Co(phen)3]4[Ge6(μ-OH)4(μ-O)2(μ-hedp)6]·2СН3СООН·30H2O by 40%. The α-galactosidase of Penicillium restrictum was not affected by the investigated compounds. Bacillus sp. IMV B-7883 elastase was activated with [Co(phen)3]4[Ge6(μ-OH)4(μ-O)2(μ-hedp)6]·2СН3СООН·30H2O by 70%, but was totally inhibited with [Fe(phen)3]4[Ge6(μ-OH)4(μ-O)2(μ-hedp)6]·20H2O. The compounds that showed the greatest 200% stimulating effect on the fibrinogenolytic activity of Bacillus sp. IMV B-7883 were [Fe(phen)3]4[Ge6(μ-OH)4(μ-O)2(μ-hedp)6]·20H2O and [Zn(phen)2(H2O)2]2[Zn(phen)(H2O)4]2[Ge6(μ-OH)4(μ-O)2(μ-hedp)6]·18H2O.

Keywords: , , , , , , , ,

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