Tag Archives: antioxidants

Antioxidants as supplements during drug-induced thrombocytopenia: a comparative analysis of Vanillic acid, L-carnitine and Caripill™

M. Mithun, V. Rajashekaraiah*

Department of Biotechnology, School of Sciences,
JAIN (Deemed-to-be University), Bengaluru, Karnataka, India;
*e-mail: vani.rs@jainuniversity.ac.in

Received: 20 September 2023; Revised: 07 November 2023;
Accepted: 01 February 2024; Available on-line: 26 February 2024

Drug-induced thrombocytopenia (DIT) is a disorder where platelet count declines as an adverse effect of therapeutic drugs. Plant extract of C. papaya Caripill™ is known to elevate platelet count under thrombocytopenic conditions. To evaluate the contribution of supplements with antioxidant potential to treat DIT, the comparative study of Caripill™, vanillic acid L-carnitine effect on platelet count and indices of oxidative stress in a model of rat thrombocytopenia induced through oral administration of hydroxyurea was performed. Wistar rats were grouped into four categories with five animals in each group: control (without any treatment); control + antioxidants; thrombocytopenia; thrombocytopenia + antioxidants. The above-mentioned antioxidants were supplemented orally at 50 mg/kg for 7 days. The level of lipid peroxidation products­, superoxides, protein carbonyls and sulfhydryls, SOD and CAT activity in isolated platelets as oxidative stress markers, and indices of platelets aggregation and ATP secretion as functional markers were used. Vanillic acid was shown to be beneficial, similar to Caripill™, during hydroxyurea-induced thrombocytopenia by maintaining platelet functions, enhancing both the antioxidant capacity of platelets and its number. L-carnitine efficiently up-regulated the enzymatic antioxidants, maintained platelet functions and protected lipids and proteins from oxidation in thrombocytopenic rats, however, it could not improve the platelet count. These findings open new avenues for employing the studied antioxidants as supplements for therapeutic purposes.

Effects of Pistacia atlantica resin oil on the level of VEGF, hydroxyproline, antioxidant and wound healing activity in STZ-induced diabetic rats

B. Shahouzehi1, M. Shabani2, N. Shahrokhi3, S. Sadeghiyan4, Y. Masoumi-Ardakani5*

1Cardiology Research Center, Institute of Basic and Clinical Physiology Sciences, Kerman University of Medical Sciences, Kerman, Iran;
e-mail: bshahouzehi@yahoo.com;
2Neuroscience Research Center, Institute of Neuropharmacology, Kerman University of Medical Sciences, Kerman, Iran;
3Department of Physiology, Afzalipour School of Medicine & Physiology Research Center, Kerman University of Medical Sciences, Kerman, Iran;
4Neuroscience Research Center, Institute of Neuropharmacology, Kerman University of Medical Sciences, Kerman, Iran;
5Physiology Research Center, Institute of Basic and Clinical Physiology Sciences, Kerman University of Medical Sciences, Kerman, Iran;
e-mail: ymab125@gmail.com

Diabetes mellitus is a chronic metabolic disorder which is followed by many complications. One of these problems following diabetes is diabetic ulcers and 2.5% of diabetic patients develop foot ulcer annually. Natural resources are useful stock for finding traditional treatment. Studies which examined Pistacia atlantica properties showed that it has some beneficial effects including: antimicrobial, antifungal, antioxidant and wound healing. Yet, Pistacia atlantica resin oil effects on diabetic wound have not been examined. Therefore, we evaluate Pistacia atlantica resin oil antioxidant and wound healing activity. Sprague-Dawley male rats were entered to the study and randomly grouped (n = 10) as follows: group 1 – control group – burnt rats which received neither STZ nor Pistacia atlantica resin oil; group 2 – diabetic burnt rats; group 3 – diabetic burnt rats which received 250 µl/day Pistacia atlantica resin oil topically as an ointment for two weeks. At the end of the study antioxidant status, Vascular Endothelial Growth Factor (VEGF) and hydroxyproline contents were examined in the wound area. Our results showed that Pistacia atlantica resin oil has remarkable antioxidant activity in STZ-induced diabetic rats. Also, it promoted VEGF and hydroxyproline contents in the wound area which showed that it increases angiogenesis and collagen turnover in the diabetic wound. We concluded that Pistacia atlantica resin oil can be considered as a new therapeutic agent in diabetic wound healing, and also it is safe, available and cheap treatment of other wounds and skin injuries.

Antioxidant activity of melatonin and glutathione interacting with hydroxyl- and superoxide anion radicals

T. Y. Kuznetsova1, N. V. Solovyova2, V. V. Solovyov1, V. O. Kostenko2

1Yu.Kondratyuk Poltava National Technical University, Ukraine;
2Ukrainian Medical Stomatological Academy, Poltava;
e-mail: kzt7@ukr.net

Based on the analysis of the results obtained by quantum chemical modeling of interaction between reduced glutathione (GSH) and melatonin (MLT) molecules with oxygen radicals (•OH and • OOˉ) it was found that this interaction occured following the acid-base mechanism, where MLT and GSH acted as a base in respect of •OH, and as acid in respect of •OOˉ. We have carried out the correlation of the results of quantum chemical calculations (density redistribution, energetic characteristics) under the interaction of MLT and GSH molecules with •OH and •OOˉ in changing macroscopic properties of the process of electroreduction of free oxygen radicals in the presence of antioxidants (potential and maximal current wave reduction waves). This was a direct experimental macroscale evidence of the results of theoretical modeling at the nanoscale level that pointed to a marked antioxidant activity of glutathione compared with melatonin.

Quantum chemical modeling of antioxidant activity of glutathione interacting with hydroxyl- and superoxide anion radicals

N. V. Solovyova1, T. Y. Kuznetsova2

1Higher State Medical Educational Institution of Ukraine,
Ukrainian Medical Stomatological Academy, Poltava, Ukraine;
2Yu. Kondratyuk Poltava National Technical University, Ukraine;
e-mail: KZT7@yandex.ru

Following the analysis of the results of quantum chemical simulation of interaction between a GSH molecule and oxygen radicals •ОН and •ООˉ, it was found that it takes place through the acid-base mechanism, where GSH acts as a base towards •ОН, and as an acid towards •ООˉ. The results of quantum chemical calculations (electron density redistribution, energy characteristics) were correlated at the time of interaction of a GSH molecule with •ОН and •ООˉ with a change of macroscopic parameters of the process of free oxygen radical electroreduction in the presence of GSH (potential and maximum current of reduction waves), which is a direct experimental macroscale evidence of results of the conducted nanoscale theoretical simulation.