Ukr.Biochem.J. 2017; Volume 89, Issue 6, Nov-Dec, pp. 22-30

doi: https://doi.org/10.15407/ubj89.06.022

Antioxidant activity of melatonin and glutathione interacting with hydroxyl- and superoxide anion radicals

T. Y. Kuznetsova1, N. V. Solovyova2, V. V. Solovyov1, V. O. Kostenko2

1Yu.Kondratyuk Poltava National Technical University, Ukraine;
2Ukrainian Medical Stomatological Academy, Poltava;
e-mail: kzt7@ukr.net

Based on the analysis of the results obtained by quantum chemical modeling of interaction between reduced glutathione (GSH) and melatonin (MLT) molecules with oxygen radicals (•OH and • OOˉ) it was found that this interaction occured following the acid-base mechanism, where MLT and GSH acted as a base in respect of •OH, and as acid in respect of •OOˉ. We have carried out the correlation of the results of quantum chemical calculations (density redistribution, energetic characteristics) under the interaction of MLT and GSH molecules with •OH and •OOˉ in changing macroscopic properties of the process of electroreduction of free oxygen radicals in the presence of antioxidants (potential and maximal current wave reduction waves). This was a direct experimental macroscale evidence of the results of theoretical modeling at the nanoscale level that pointed to a marked antioxidant activity of glutathione compared with melatonin.

Keywords: , , , ,


References:

  1. Parasuraman S, Maithili KS. Antioxidant and drug metabolism. Free Rad Antiox. 2014; 4(1):01-02. CrossRef
  2. What Are Free Radicals? [Electronic resource]. − Access mode//https://www. globalhealingcenter.com/natural-health/what-are-free-radicals/.
  3. Lobo V, Patil A, Phatak A, Chandra N. Free radicals, antioxidants and functional foods: Impact on human health. Pharmacogn Rev. 2010 Jul;4(8):118-26. PubMed, PubMedCentral, CrossRef
  4. Sparman A, Thompson K. Safety Profile and Effect on Libido of a Combined Bryophyllum pinnatum, Moringa oleifera and Vitamin C Phytotherapeutic Agent. Free Rad Antiox. 2017;7(2):156-159.  CrossRef
  5. Naidu JN, Swapna GN, Kumar AN, Krishnamma M, Anitha M. Importance of elevated insulin resistance, dyslipidemia and status of antioxidant vitamins in polycystic ovary disease.  Free Rad Antiox. 2013; 3(1):17-19.  CrossRef
  6. Belenichev IF, Gubsky YuI, Levitsky EL, Kovalenko SI, Marchenko AN. Regulation of antioxidant homeostasis and system detoxication of organizm by hormone melatonin. Role of melatonin dependent receptors in realization of this function. Modern Probl Toxicol. 2003;(2):2-16. (In Russian).
  7. Malinovskaya NK. The role of melatonin in the human body. Klin Med. 1998;(10):15-22. (In Russian).
  8. Dawson D, van den Heuvel CJ. Integrating the actions of melatonin on human physiology. Ann Med. 1998 Feb;30(1):95-102. PubMed, CrossRef
  9. Kvetnaya TV, Knyazkin IV. Melatonin: role and importance in age-related pathology. St. Petersburg, 2003. 93 p. (In Russian)
  10. Reiter RJ. Melatonin: Lowering the High Price of Free Radicals. News Physiol Sci. 2000 Oct;15:246-250. PubMed
  11. Bachurin SO.  Medicinal and chemical approaches to focused search of agents for treatment and therapy of Alzheimer disease. Vopr Med Khim. 2001 Mar-Apr;47(2):155-97. (In Russian). PubMed
  12. Kuznetsova TY, Solovyova NV. Simulation of antioxidant properties of melatonin and glutathione in contact with hydroxyl radicals. Actual Probl Mod Med. News Ukr Med Stomat Acad. 2012;12(1-2): 189-193. (In Russian).
  13. Solovyova NV, Kuznetsova TY. Quantum chemical modeling of antioxidant activity of glutathione interacting with hydroxyl- and superoxide anion radicals. Ukr Biochem J. 2015 Mar-Apr;87(2):156-62. PubMed, CrossRef
  14. Magin DV, Izmaylov DIu, Popov IN, Levin G, Vladimirov IuA. Photochemiluminescent study of the antioxidant activity in biological systems. Mathematical modeling. Vopr Med Khim. 2000 Jul-Aug;46(4):419-25. (In Russia). PubMed
  15. Ehlenfeldt MK, Prior RL. Oxygen radical absorbance capac ity (ORAC) and phenolic and anthocyanin concentrations in fruit and leaf tissues of highbush blueberry. J Agricult Food Chem. 2001;49(5): 2222-2227. CrossRef
  16. Korotkova EI, Karbainov YA, Shevchuk AV. Study of antioxidant properties by voltammetry. J Electroanal Chem. 2002;518(1):56-60. CrossRef
  17. Korotkova EI, Karbainov YA, Avramchik OA. Investigation of antioxidant and catalytic properties of some biologically active substances by voltammetry. Anal Bioanal Chem. 2003 Feb;375(3):465-8.  PubMed, CrossRef
  18. Prütz WA, Butler J, Land EJ. The glutathione free radical equilibrium, GS. + GS−⇌ GSS.−G, mediating electron transfer to FE(III) -cytochrome c. Biophys Chem. 1994; 49(2):101-111.  CrossRef
  19. General Atomic and Molecular Electronic Structure System. [Electronic resource]. − Access mode //http://www.msg.ameslab.gov /gamess/.
  20. Alex A. Granovsky. Firefly and PC GAMESS /Firefly version 8.0.1. [Electronic resource]. − Access mode //http://classic.chem.msu.su/gran /gamess/index.html.
  21. Shapoval GS, Kruglyak OS, Kuznetsova TYu, Soloviev VV, Nestyuk NV. Electrocatalytic oxidation of natural endogenous antioxidants. Catal Petrochem. 2009;(17): 31-38. (In Russian).

Creative CommonsThis work is licensed under a Creative Commons Attribution 4.0 International License.