Tag Archives: bile

Corvitin modulates the content of lipids in rat liver bile

T. V. Vovkun1, P. I. Yanchuk1, L. Ya. Shtanova1,
S. P. Veselsky1, N. B. Filimonova1, I. V. Komarov2

1ESC “Institute of Biology and Мedicine”, National Taras Shevchenko University of Kyiv, Ukraine
2“Institute of High Technologies
”, National Taras Shevchenko University of Kyiv, Ukraine;
e-mail: shtanova@ukr.net

Received: 23 March 2019; Accepted: 18 October 2019

Quercetin (QUE) and its water-soluble form сorvitin are medicinally important members of the fla­vonoid family and the most prominent dietary antioxidants. Numerous pharmacological effects of quercetin include protection against diseases, such as atherosclerosis, myocardial infarction, and cerebrovascular disea­ses. Corvitin modulates liver blood flow but its effects on liver lipid metabolism have not been understood. We investigated the influence of corvitin (2.5, 5, 10 mg/kg) on the formation and secretion of cholesterol (Chol), cholesterol esters (EChol), phospholipids (PLs), free fatty acids (FFAs), and triglycerides (TGs) into the bile. Secreted bile was collected during 2.5 h of the experiment. Lipid fractions of the bile were separated by thin-layer chromatography. We defined that administration of corvitin caused a significant (P < 0.05–0.001) increase in levels of all studied lipid components of bile. At a dose of 2.5 and 5 mg/kg corvitin increased bile content of Chol, PLs and FFAs,and at a  dose of 10 mg/kg had the largest effect on the total production of EChol and TGs. We concluded that corvitin activates the liver lipids metabolism and processes of bile formation.

Correlations of bile acids in the bile of rats in conditions of alloxan induced diabetes melitus

N. N. Danchenko, S. P. Veselskiy, B. А. Tsydzevich

Taras Shevchenko National University of Kyiv, ESC Institute of Biology, Ukraine;
e-mail: ninadanc@gmail.com

The ratio of bile acids in the bile of rats with alloxan diabetes was investigated using the method of thin-layer chromatography. Changes of coefficients of conjugation and hydroxylation of bile acids were calculated and analyzed in half-hour samples of bile obtained during the 3-hour experiment. It has been found that the processes of conjugation of cholic acid with glycine and taurine are inhibited in alloxan diabetes. At the same time a significant increase of free threehydroxycholic and dіxydroxycholic bile acids and conjugates of the latter ones with  taurine has been registered. Coefficients of hydroxylation іn alloxan diabetes show the domination of “acidic” pathway in bile acid biosynthesis that is tightly connected with the activity of mitochondrial enzymes.