Tag Archives: carcinogenesis

Scientific and practical activity of the Laboratory of Cell Signaling Mechanisms of the Palladin Institute of Biochemistry of NAS of Ukraine

R. P. Vynogradova, I. Yu. Chernysh, V. M. Danilova

Palladin Institute of Biochemistry, National Academy of Sciences of Ukraine, Kyiv;
e-mail: valdan@biochem.kiev.ua

The article is devoted to the analysis of the scientific and practical activity of the laboratory of the signaling mechanisms of the cells of the Palladin Institute of Biochemistry, NAS of Ukraine, in the context of the history of its development. The most important results of studies of the mechanisms controlling proliferation, migration and invasion of tumor cells, which testify to the important role of the Ruk/CIN85 adapter protein in carcinogenesis, are presented. These studies are a priority and can serve as an experimental basis for the development of new generation pharmacological agents, the targets for which can be key centers for the organization of signaling systems of the cell – adapter and scaffold proteins.

Reactive oxygen species in signal transduction

L. B. Drobot1, A. A. Samoylenko1, A. V. Vorotnikov2, P. A. Tyurin-Kuzmin2,
A. V. Bazalii1, T. Kietzmann3, V. A. Tkachuk2, S. V. Komisarenko1

1Palladin Institute of Biochemistry, National Academy of Sciences of Ukraine, Kyiv;
e-mail: drobot@biochem.kiev.ua
2Lomonosov Moscow State University, Faculty of Basic Medicine, Russia;
3Department of Biochemistry and Biocenter Oulu, University of Oulu, Oulu, Finland;

Reactive oxygen species (ROS) are products of incomplete reduction of oxygen both nonradicals and radicals that function as mediators of redox signaling and oxidative stress depending on their levels in different­ subcellular compartments. Up to date, a huge body of data are accumulated, which supports a role of ROS as “second messengers” in intracellular signaling cascades that control cell growth, proliferation, apoptosis as well as migration and invasion. The current review summarizes data regarding ROS-dependent regulation of signaling­ networks components including MAPK, PI3K/Akt, PKC, NF-κB, Nrf2, FoxO and HIF-1α, and role of ROS in tumorigenesis.

Effect of dihydropyrrol and maleimide derivatives on the state of the liver and colon in normal rats and those with colorectal carcinogenesis induced by dimethylhydrazine

H. M. Kuznietsova, O. V. Lynchak, M. O. Danylov, I. P. Kotlyar, V. K. Rybalchenko

Taras Shevchenko National University of Kyiv, Ukraine;
e-mail: gala_kuznetsova@rambler.ru

No liver and colon alterations in rats, caused by cytostatic compounds 5-amino-4-(1,3-benzothyazol-2-yl)-1-(3-methoxyphenyl)-1,2-dihydro-3Н-pyrrol-3-one (D1) and 1-(4-Cl-benzyl)-3-Cl-4-(CF3-phenylamino)-1H-pyrrol-2,5-dione (MI-1) when administered over a long time were found, as evidenced by the histopathological data and the data of activity of transaminases, alkaline phosphatase and lactate dehydrogenase in the blood serum. D1 and MI-1 in vivo decrease the total area of DMH-induced colon tumors in rats by 46-60%. Furthermore, D1 and MI-1 partially protect the liver and colon mucosa from toxic effects caused by 1,2-dimethylhydrazine (DMH) reducing DNA oxidative modifications, as evidenced by urine 8-hydroxydeoxyguanosine level. The effects of both compounds are similar, but MI-1 is less toxic for the liver and colon of intact animals possessing more pronounced antitumor activity and protective properties in the setting of chemically induced carcinogenesis.