Tag Archives: cortisol

Black tea and coffee impact on steroid hormones status in young men

M. F. Amirova*, A. R. Dadashova, E. E. Huseynova, I. A. Kerimova,
Sh. I. Hasanova, F. E. Guliyeva, S. R. Guliyeva,
R. R. Rahimova, G. R. Vahabova

Biochemistry Department, Azerbaijan Medical University, Baku, Azerbaijan;
*e-mail: gerayelmira@gmail.com

Received: 12 June 2022; Revised: 04 August 2022;
Accepted: 04 November 2022; Available on-line: 14 November 2022

Tea and coffee alkaloids affect the hormonal status of the body. There are reports about the effect of caffeine on the body under stress, but nearly absent reports on the effect of tea and coffee alkaloids at rest. The aim of this work was to determine whether there is a significant difference in testosterone and cortisol concentrations in the blood of young men before and after drinking indicated beverages. The work was carried out on 21 healthy young males that were tested for blood cortisol levels before and after drinking tea or coffee on an empty stomach. The young men were divided into two groups: the first group comprised those whose cortisol levels increased after taking a single dose of tea, the second group – whose cortisol decreased. The third group comprised persons who took a single dose of strong-grain coffee. In addition, adrenaline and testosterone levels were determined as a hormonal panel. Our pioneer investigation found that coffee causes a significant decrease in cortisol levels at rest, but tea consumption by coffee drinkers leads to a more pronounced decrease in the cortisol levels than coffee.

Male and female rats differ in homeostatic shifts during pre-slaughter fear stress

S. S. Grabovskyi1, R. R. Panchuk2, N. R. Skorokhyd2, R. S. Stoika2*

1Stepan Gzhytskyi National University of Veterinary Medicine and Biotechnologies, Lviv, Ukraine;
2Institute of Cell Biology, National Academy of Sciences of Ukraine, Lviv;
*e-mail: stoika.rostyslav@gmail.com

Received: 19 June 2020; Accepted: 17 December 2020

In this investigation, the level of сortisol in blood plasma and  splenocytes apoptosis in male and female rats in response to fear stress created by animals’ waiting for different terms (20, 40, 60 min) before the slaughter were evaluated. A significant and dependent on the stress state duration increase in the level of cortisol in the blood plasma of  both male and female rats compared with the rats of control groups was found, which was more pronounced in females compared to males. The number of Annexin V-positive (apoptotic) cells was determined by FACS analysis. It was shown that the content of apoptotic splenocytes during the stress before slaughter was higher in females compared to males. The prolongation of  the stress period was accompanied by an increase in the content of apoptotic splenocytes in males and its decrease in females. The potential hormone-dependent attenuation of the mechanisms of adaptation to  stress before slaughter in female rats is discussed.