Ukr.Biochem.J. 2021; Volume 93, Issue 1, Jan-Feb, pp. 68-74

doi: https://doi.org/10.15407/ubj93.01.068

Male and female rats differ in homeostatic shifts during pre-slaughter fear stress

12.02.2021   Uncategorized   No comments

S. S. Grabovskyi1, R. R. Panchuk2, N. R. Skorokhyd2, R. S. Stoika2*

1Stepan Gzhytskyi National University of Veterinary Medicine and Biotechnologies, Lviv, Ukraine;
2Institute of Cell Biology, National Academy of Sciences of Ukraine, Lviv;
*e-mail: stoika.rostyslav@gmail.com

Received: 19 June 2020; Accepted: 17 December 2020

In this investigation, the level of сortisol in blood plasma and  splenocytes apoptosis in male and female rats in response to fear stress created by animals’ waiting for different terms (20, 40, 60 min) before the slaughter were evaluated. A significant and dependent on the stress state duration increase in the level of cortisol in the blood plasma of  both male and female rats compared with the rats of control groups was found, which was more pronounced in females compared to males. The number of Annexin V-positive (apoptotic) cells was determined by FACS analysis. It was shown that the content of apoptotic splenocytes during the stress before slaughter was higher in females compared to males. The prolongation of  the stress period was accompanied by an increase in the content of apoptotic splenocytes in males and its decrease in females. The potential hormone-dependent attenuation of the mechanisms of adaptation to  stress before slaughter in female rats is discussed.

Keywords: , , ,

References:

  1. Grabovskyi S, Grabovska O, Havryliak V, Kalachniuk L, Velyka A. Pre-slaughter stress affects cortisol and adrenocorticotropic hormone levels in the blood of animals. Biologija. 2019;65(3): 174-183.  CrossRef
  2. Grabovskyi SS, Kyryliv JI, Grabovska OS. The effect of consumption of broiler chickens meat іn conditions of pre-slaughter stress with іts сorrection on natural resistance of people. Acta Sci Pol Zootechnica. 2015;14(4) 55-64.
  3. Rahal A, Kumar A, Singh V, Yadav B, Tiwari R, Chakraborty S, Dhama K. Oxidative Stress, Prooxidants, and Antioxidants: The Interplay. BioMed Res Int. 2014;2014:ID761264. CrossRef
  4. Vélez-Marín M, Hurtado Salazar A, Uribe-Velásquez LF. Plasma cortisol activity in rats under conditions of chronic stress supplemented with resveratrol. Colomb Med (Cali). 2012;43(3):221-225. PubMed, PubMedCentral, CrossRef
  5. Vaziri ND, Lin CY, Farmand F, Sindhu RK. Superoxide dismutase, catalase, glutathione peroxidase and NADPH oxidase in lead-induced hypertension. Kidney Int. 2003;63(1):186-194. PubMed, CrossRef
  6. Gruver-Yates AL, Cidlowski JA. Tissue-specific actions of glucocorticoids on apoptosis: a double-edged sword. Cells. 2013;2(2):202-223. PubMed, PubMedCentral, CrossRef
  7. Wang LI, Liu F, Luo Y, Zhu L, Li G. Effect of acute heat stress on adrenocorticotropic hormone, cortisol, interleukin-2, interleukin-12 and apoptosis gene expression in rats. Biomed Rep. 2015;3(3):425-429. PubMed, PubMedCentral, CrossRef
  8. Golab J, Jakobisiak M, Lasek W, Stoklosa T. Immunologia (nowe wydanie). Warszawa, Wydawnictwo Naukowe PWN SA, 2017. 497 p.
  9. Peeva E, Zouali M. Spotlight on the role of hormonal factors in the emergence of autoreactive B-lymphocytes. Immunol Lett. 2005;101(2):123-143. PubMed, CrossRef
  10. Jameel MK, Joshi AR. Effect of acute stress on serum cortisol level in female wistar rats. Int J Healthc Biomed Res. 2015;03(04):109-113.
  11. Bereshchenko O, Bruscoli S Riccardi C. Glucocorticoids, Sex Hormones, and Immunity. Front Immunol. 2018;9:1332. PubMed, PubMedCentral, CrossRef
  12. Tang D, Kang R, Berghe TV, Vandenabeele P, Kroemer G.  The molecular machinery of regulated cell death. Cell Res. 2019;29(5):347-364.  PubMed, PubMedCentral, CrossRef
  13. Sancho Cantus D, Santiesteban López N, Cuerda Ballester M, Gómez S, Enriquede la Rubia Ortí J. Stress in Parkinson’s disease. Cortisol and amylase biomarkers. Systematic review. Revista Científica de la Sociedad de Enfermería Neurológica. 2019; 50: 12-22.  CrossRef
  14. Glenk LM, Kothgassner OD, Felnhofer A, Gotovina J, Pranger CL, Jensen AN, Mothes-Luksch N, Goreis A, Palme R, Jensen-Jarolim E. Salivary cortisol responses to acute stress vary between allergic and healthy individuals: the role of plasma oxytocin, emotion regulation strategies, reported stress and anxiety. Stress. 2020;23(3):275-283.  PubMed, PubMedCentral, CrossRef
  15. Griebe M, Ebert A, Nees F, Katic K, Gerber B, Szabo K. Enhanced cortisol secretion in acute transient global amnesia. Psychoneuroendocrinology. 2019;99:72-79. PubMed, CrossRef
  16. Huang J, Cui H, Peng X, Fang J, Zuo Z, Deng J, Wu B. The association between splenocyte apoptosis and alterations of Bax, Bcl-2 and caspase-3 mRNA expression, and oxidative stress induced by dietary nickel chloride in broilers. Int J Environ Res Public Health. 2013;10(12):7310-7326.  PubMed, PubMedCentral, CrossRef
  17. Chen W, Ni J, Qiao Z, Wu Y, Lu L, Zheng J, Chen R, Lu X. Comparison of the Clinical Outcomes of Two Physiological Ischemic Training Methods in Patients with Coronary Heart Disease. Open Med (Wars). 2019;14:224-233. PubMed, PubMedCentral, CrossRef
  18. Gong S, Miao YL, Jiao GZ, Sun MJ, Li H, Lin J, Luo MJ, Tan JH. Dynamics and correlation of serum cortisol and corticosterone under different physiological or stressful conditions in mice. PLoS One. 2015;10(2):e0117503. PubMed, PubMedCentral, CrossRef
Creative CommonsThis work is licensed under a Creative Commons Attribution 4.0 International License.