Tag Archives: DPPH
Isolation, characterization and antioxidant activity of fibrinogen-like protein-1 from serum and synovial fluid of patients with rheumatoid arthritis
Abdulsattar J. Abdullah, Zahraa M. A. Hamodat*
Department of Chemistry, College of Science, University of Mosul, Iraq;
*e-mail: zahraahamodat@uomosul.edu.iq
Received: 20 April 2025; Revised: 01 June 2025;
Accepted: 11 June 2025; Available on-line: 07 July 2025
Rheumatoid arthritis (RA) is an autoimmune disease characterized by chronic inflammation and oxidative stress. Fibrinogen-like protein-1 (FGL1) has been implicated in immune regulation, but its antioxidant role under inflammatory conditions remains underexplored. This study aimed to isolate and purify FGL1 from the serum of healthy controls and from the serum and synovial fluid from inflamed joints of RA patients, and to assess its antioxidant capacity. Purification included ammonium sulfate precipitation (65%), dialysis, and gel filtration chromatography (Sephadex G-75), SDS-PAGE and HPLC. Antioxidant activity was evaluated by DPPH radical scavenging and IC50 calculation. SDS-PAGE and HPLC analysis confirmed the successful isolation, identity and high purity of FGL1 from all samples, the protein molecular weight ranged from 68 to 70 kDa. The DPPH assay showed that FGL1 isolated from synovial fluid of RA patients had the highest antioxidant activity (IC50 = 2.124 ng/ml), followed by RA serum (2.172 ng/ml) and control serum (2.798 ng/ml). These results indicate the dual role of FGL1 protein in immune response and oxidative balance, making it a promising biomarker and potential therapeutic target in rheumatoid arthritis.
Evaluation of the spectral characteristics, purity and antioxidant activity of C-phycocyanin from the cyanobacteria collected in Kaunas Lagoon (Lithuania)
N. Hudz1,2*, V. Turkina3, O. Yezerska1, L. Kobylinska4, A. Filipska1,
J. Karosienė5, D. Galinytė6, G. Balčiūnaitė–Murzienė7,
S. Khomyak8, N. Savickienė9
1Department of Drug Technology and Biopharmacy,
Danylo Halytsky Lviv National Medical University, Lviv, Ukraine;
2Department of Pharmacy and Ecological Chemistry, University of Opole, Poland;
3Research Institute of Epidemiology and Hygiene,
Danylo Halytsky Lviv National Medical University, Lviv, Ukraine;
4Department of Biochemistry, Danylo Halytsky Lviv National Medical University, Lviv, Ukraine;
5Laboratory of Algology and Microbial Ecology, Nature Research Centre, Vilnius, Lithuania;
6Department of Pharmacognosy, Lithuanian University of Health Sciences, Kaunas, Lithuania;
7Institute of Pharmaceutical Technologies, Lithuanian University of Health Sciences, Kaunas, Lithuania;
8Department of Technology of Biologically Active Substances, Pharmacy and Biotechnology,
Lviv Polytechnic National University, Lviv, Ukraine;
9Department of Pharmacognosy, Lithuanian University of Health Sciences, Kaunas, Lithuania;
*e-mail: natali_gudz@ukr.net
Received: 09 October 2022; Revised: 14 November 2022;
Accepted: 16 November 2022; Available on-line: 19 December 2022
The physicochemical characteristics of phycocyanin extracted from cyanobacteria collected in Kaunas Lagoon were studied (spectrum characteristics, C-PC content in the dry mass and chemical purity). It was determined that the tested concentrations of C-PC in purified water should be in the range of 0.02–0.16% for measuring C-PC content in the dry mass and its spectrum characteristics. The two clear absorption maxima were detected in the spectrum of C-PC at the wavelengths of 277 and 619 nm. The content of C-PC in the dry powder form was in the range of 7.25% to 9.30% depending on its concentration in the solution and type of spectrophotometer. Furthermore, a purity factor of 1.5 was calculated, which indicated the food qualification of the obtained biomass of C-PC. Finally, the analytical procedure for studying the pro- and anti-oxidant activity of C-PC was developed and the antioxidant activity of C-PC was measured for the available markers. It was revealed that C-PC has dual properties (pro- and anti-oxidant ones) depending on its concentration, more exactly, its content in reaction mixtures with 2,2-diphenyl-1-picrylhydrazyl (DPPH). The following issues were resolved during the research: the concentration of ethanol in the DPPH solution was chosen in order to avoid precipitation of proteins in the reaction mixtures (50%); the ratio of the solution of C-PC to the DPPH solution was selected; the selected concentrations of the markers for the construction of their calibration curves were chosen for quercetin and for rutin. The antioxidant activity of the obtained C-PC sample was determined.