Ukr.Biochem.J. 2022; Volume 94, Issue 5, Sep-Oct, pp. 47-58

doi: https://doi.org/10.15407/ubj94.05.047

Evaluation of the spectral characteristics, purity and antioxidant activity of C-phycocyanin from the cyanobacteria collected in Kaunas Lagoon (Lithuania)

N. Hudz1,2*, V. Turkina3, O. Yezerska1, L. Kobylinska4, A. Filipska1,
J. Karosienė5, D. Galinytė6, G. Balčiūnaitė–Murzienė7,
S. Khomyak8, N. Savickienė9

1Department of Drug Technology and Biopharmacy,
Danylo Halytsky Lviv National Medical University, Lviv, Ukraine;
2Department of Pharmacy and Ecological Chemistry, University of Opole, Poland;
3Research Institute of Epidemiology and Hygiene,
Danylo Halytsky Lviv National Medical University, Lviv, Ukraine;
4Department of Biochemistry, Danylo Halytsky Lviv National Medical University, Lviv, Ukraine;
5Laboratory of Algology and Microbial Ecology, Nature Research Centre, Vilnius, Lithuania;
6Department of Pharmacognosy, Lithuanian University of Health Sciences, Kaunas, Lithuania;
7Institute of Pharmaceutical Technologies, Lithuanian University of Health Sciences, Kaunas, Lithuania;
8Department of Technology of Biologically Active Substances, Pharmacy and Biotechnology,
Lviv Polytechnic National University, Lviv, Ukraine;
9Department of Pharmacognosy, Lithuanian University of Health Sciences, Kaunas, Lithuania;
*e-mail: natali_gudz@ukr.net

Received: 09 October 2022; Revised: 14 November 2022;
Accepted: 16 November 2022; Available on-line: 19 December 2022

The physicochemical characteristics of phycocyanin extracted from cyanobacteria collected in Kaunas Lagoon were studied (spectrum characteristics, C-PC content in the dry mass and chemical purity). It was determined that the tested concentrations of C-PC in purified water should be in the range of 0.02–0.16% for measuring C-PC content in the dry mass and its spectrum characteristics. The two clear absorption maxima were detected in the spectrum of C-PC at the wavelengths of 277 and 619 nm. The content of C-PC in the dry powder form was in the range of 7.25% to 9.30% depending on its concentration in the solution and type of spectrophotometer. Furthermore, a purity factor of 1.5 was calculated, which indicated the food qualification of the obtained biomass of C-PC. Finally, the analytical procedure for studying the pro- and anti-oxidant activity of C-PC was developed and the antioxidant activity of C-PC was measured for the available markers. It was revealed that C-PC has dual properties (pro- and anti-oxidant ones) depending on its concentration, more exactly, its content in reaction mixtures with 2,2-diphenyl-1-picrylhydrazyl (DPPH). The following issues were resolved during the research: the concentration of ethanol in the DPPH solution was chosen in order to avoid precipitation of proteins in the reaction mixtures (50%); the ratio of the solution of C-PC to the DPPH solution was selected; the selected concentrations of the markers for the construction of their calibration curves were chosen for quercetin and for rutin. The antioxidant activity of the obtained C-PC sample was determined.

Keywords: , , , , ,


References:

  1. Izadi M, Fazilati M. Extraction and purification of phycocyanin from Spirulina platensis and evaluating its antioxidant and anti-inflammatory activity. Asian J Green Chem. 2018;2(4):364-379. CrossRef
  2. Patel HM, Rastogi RP, Trivedi U, Madamwar D. Structural characterization and antioxidant potential of phycocyanin from the cyanobacterium Geitlerinema sp. H8DM. Algal Res. 2018;32:372-383. CrossRef
  3. Chentir I, Hamdi M, Li S, Doumandji A, Markou G, Nasri M. Stability, bio-functionality and bio-activity of crude phycocyanin from a two-phase cultured Saharian Arthrospira sp. strain. Algal Res. 2018;35:395-406. CrossRef
  4. Chiu HF, Yang SP, Kuo YL, Lai YS, Chou TC. Mechanisms involved in the antiplatelet effect of C-phycocyanin. Br J Nutr. 2006;95(2):435-440.
    PubMed, CrossRef
  5. de Amarante MCA, Braga ARC, Sala L, Kalil SJ. Colour stability and antioxidant activity of C-phycocyanin-added ice creams after in vitro digestion. Food Res Int. 2020;137:109602. PubMed, CrossRef
  6. Gantar M, Simović D, Djilas S, Gonzalez WW, Miksovska J. Isolation, characterization and antioxidative activity of C-phycocyanin from Limnothrix sp. strain 37-2-1. J Biotechnol. 2012;159(1-2):21-26. PubMed, PubMedCentral, CrossRef
  7. Hirata T, Tanaka M, Ooike M, Tsunomura T, Sakaguchi M. Antioxidant activities of phycocyanobilin prepared from Spirulina platensis. J Appl Phycol. 2000;12(3):435-439. CrossRef
  8. Jiang L, Wang Y, Yin Q, Liu G, Liu H, Huang Y, Li B. Phycocyanin: A Potential Drug for Cancer Treatment. J Cancer. 2017;8(17):3416-3429. PubMed, PubMedCentral, CrossRef
  9. Safari R, Raftani Amiri Z, Esmaeilzadeh Kenari R. Antioxidant and antibacterial activities of C-phycocyanin from common name Spirulina platensis. Iran J Fish Sci. 2013;19(4):1911-1927. CrossRef
  10. Hudz NI, Turkina VA, Filipska AM, Kuzminov OB, Korytniuk RS, Lubenets VI, Wieczorek PP, Savickiene N. Protein c-phycocyanin, structure, physicochemical and biological properties, methods of extraction. Biopolym Cell. 2021;37(6):407-418. CrossRef
  11. Park WS, Kim HJ, Li M, Lim DH, Kim J, Kwak SS, Kang CM, Ferruzzi MG, Ahn MJ. Two Classes of Pigments, Carotenoids and C-Phycocyanin, in Spirulina Powder and Their Antioxidant Activities. Molecules. 2018;23(8):2065. PubMed, PubMedCentral, CrossRef
  12. Aoki J, Sasaki D, Asayama M. Development of a method for phycocyanin recovery from filamentous cyanobacteria and evaluation of its stability and antioxidant capacity. BMC Biotechnol. 2021;21(1):40. PubMed, PubMedCentral, CrossRef
  13. Finamore A, Palmery M, Bensehaila S, Peluso I. Antioxidant, Immunomodulating, and Microbial-Modulating Activities of the Sustainable and Ecofriendly Spirulina. Oxid Med Cell Longev. 2017;2017:3247528. PubMed, PubMedCentral, CrossRef
  14. Wu HL, Wang GH, Xiang WZ, Li T, He H. Stability and antioxidant activity of food-grade phycocyanin isolated from Spirulina platensis. Int J Food Prop. 2016;19(10):2349–2362. CrossRef
  15. Prez J, Alejandro T. Chemistry of natural antioxidants and studies performed with different plants collected in Mexico. In book: Oxidative Stress and Chronic Degenerative Diseases – A Role for Antioxidants. Ed. Morales-González J.A. InTech: Rijeka, Croatia, 2013; P. 59-85. CrossRef
  16. Agrawal M, Bansal S, Chopra K. Evaluation of the in vitro and in vivo antioxidant potentials of food grade Phycocyanin. J Food Sci Technol. 2021;58(11):4382-4390. PubMed, PubMedCentral, CrossRef
  17. Liang N, Kitts DD. Antioxidant property of coffee components: assessment of methods that define mechanisms of action. Molecules. 2014;19(11):19180-19208. PubMed, PubMedCentral, CrossRef
  18. Asatiani MD, Elisashvili VI, Wasser SP, Reznick AZ, Nevo E. Free-radical scavenging activity of submerged mycelium extracts from higher basidiomycetes mushrooms. Biosci Biotechnol Biochem. 2007;71(12):3090-3092. PubMed, CrossRef
  19. Gabr GA, El-Sayed SM, Hikal MS. Antioxidant activities of phycocyanin: A bioactive compound from Spirulina platensis. J Pharm Res Int. 2020;32(2):73-85. CrossRef
  20. Khazi MI, Demirel Z, Conk Dalay M. Enhancement of biomass and phycocyanin content of Spirulina platensis. Front Biosci. 2018;10(2):276-286. PubMed, CrossRef
  21. Garcia AB, Longo E, Bermejo R. The application of a phycocyanin extract obtained from Arthrospira platensis as a blue natural colorant in beverages. J Appl Phycol. 2021;33(5):3059-3070. CrossRef
  22. Minato T, Teramoto T, Adachi N, Hung NK, Yamada K, Kawasaki M, Akutsu M, Moriya T, Senda T, Ogo S, Kakuta Y, Yoon KS. Non-conventional octameric structure of C-phycocyanin. Commun Biol. 2021;4(1):1238. PubMed, PubMedCentral, CrossRef
  23. Safaei M, Maleki H, Soleimanpour H, Norouzy A, Zahiri HS, Vali H, Noghabi KA. Development of a novel method for the purification of C-phycocyanin pigment from a local cyanobacterial strain Limnothrix sp. NS01 and evaluation of its anticancer properties. Sci Rep. 2019;9(1):9474. PubMed, PubMedCentral, CrossRef
  24. Venugopal VC, Thakur A, Chennabasappa LK, Mishra G, Singh K, Rathee P, Ranjan A. Phycocyanin Extracted from Oscillatoria minima Shows Antimicrobial, Algicidal, and Antiradical Activities: In silico and In vitro Analysis. Antiinflamm Antiallergy Agents Med Chem. 2020;19(3):240-253. PubMed, PubMedCentral, CrossRef
  25. Kamble SP, Gaikar RB, Padalia RB, Shinde KD. Extraction and purification of C-phycocyanin from dry Spirulina powder and evaluating its antioxidant, anticoagulation and prevention of DNA damage activity. J Appl Pharm Sci. 2013;3(8):149-153. CrossRef
  26. Khandual S, Sanchez EOL, Andrews HE, de la Rosa JDP. Phycocyanin content and nutritional profile of Arthrospira platensis from Mexico: efficient extraction process and stability evaluation of phycocyanin. BMC Chem. 2021;15(1):24. PubMed, PubMedCentral, CrossRef
  27. Panchal J, Shrivastav A. Extraction and purification of phycocyanin from spirulina platensis by using different methods: A Review. Int J Biol Pharm Allied Sci. 2020;9(7):1653-1669. CrossRef
  28. Soni A, Dubey M, Verma M, Dhankhar R, Kaushal V, Atri R, Sabharwal R. Revisiting the role of phycocyanin in current clinical practice. Int J Pharm Sci Res. 2015;6(11):4950–4600. CrossRef
  29. Hudz N, Ivanova R, Brindza J, Grygorieva O, Schubertová Z, Ivanišová E. Approaches to the determination of antioxidant activity of extracts from bee bread and safflower leaves and flowers. Potr S J F Sci. 2017;11(1):480–488. CrossRef
  30. Shanaida M, Hudz N, Jasicka-Misiak I, Wieczorek PP. Polyphenols and Pharmacological Screening of a Monarda fistulosa L. dry Extract Based on a Hydrodistilled Residue By-Product. Front Pharmacol. 2021;12:563436. PubMed, PubMedCentral, CrossRef
  31. Kobylinska L, Klyuchivska O, Lesyk R, Stoika R. Targeting of the pro-oxidant-antioxidant balance in vitro and in vivo by 4-thiazolidinone-based chemotherapeutics with anticancer potential. Ukr Biochem J. 2019;91(2):7-17. CrossRef
  32. Hudz N, Yezerska O, Grygorieva O, Brindza J, Felšöciová S, Kačániová M, Wieczorek PP. Analytical procedure elaboration of total flavonoid content determination and antimicrobial activity of bee bread extracts. Acta Pol Pharm. 2019;76(3):439–452. CrossRef
  33. Daniel WW. Biostatistics: A Foundation for Analysis in the Health Sciences, 9th ed.; Wiley: Hoboken, NJ, USA. 2009. P. 215-244.
  34. Song W, Zhao C, Wang S. A large-scale preparation method of high purity C-phycocyanin. Int J Biosci Biochem Bioinforma. 2013;3(4):293-297. CrossRef
  35. Hadiyanto, Suzery M, Setyawan D, Majid D, Sutanto H. Encapsulation of phycocyanin-alginate for high stability and antioxidant activity. IOP Conf Ser: Earth Environ Sci. 2017;55:012030. CrossRef
  36. Zhou ZP, Liu LN, Chen XL, Wang JX, Chen MIN, Zhang YZ, Zhou BC. Factors that effect antioxidant activity of C‐phycocyanins from Spirulina platensis. J Food Biochem. 2005;29(3):313-322. CrossRef
  37. Hudz N, Sedláčková VH, Yezerska O, Zhurba M, Brindza J. Methodological approach to the elaboration of the analytical procedure of the antioxidant activity determination of the Schisandra chinensis (Turcz) Baill. extracts. Agrobiodivers Improv Nutr Health Life Qual. 2021;5(2):250-257.
    CrossRef

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