Tag Archives: glycogen

The Gerty Cori phenomenon: the first woman Nobel laureate in Physiology and Medicine

26.07.2024   Uncategorized   No comments

V. M. Danilova, M. V. Grigorieva, S. V. Komisarenko

Palladin Institute of Biochemistry, National Academy of Sciences of Ukraine, Kiyv;
e-mail: valdan@biochem.kiev.ua

Received: 08 June 2024; Revised: 08 July 2024;
Accepted: 25 July 2024; Available on-line: 04 September 2024

Gerty Cori was the first woman awarded the Nobel Prize in Physiology or Medicine, the first woman the Nobel Prize laureate in the United States, and the third woman the Nobel Prize laureate worldwide following Marie Curie-Skłodowska and her daughter, Irene Joliot-Curie. Despite a challenging and lengthy path to recognition, Gerty Cori’s exceptional qualities and strong character led to her success. In 1947, she was awarded the Nobel Prize in Physiology or Medicine jointly with her husband and lifelong research partner Carl Cori “for their discovery of the course of the catalytic conversion of glycogen”. The Nobel Committee recognized this discovery as one of the most outstanding achievements in biochemistry.

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Developmental diet defines metabolic traits in larvae and adult Drosophila

02.05.2022   Uncategorized   No comments

O. M. Strilbytska1*, U. V. Semaniuk1, N. I. Burdyliyk1, V. Bubalo2, O. V. Lushchak1,3*

1Department of Biochemistry and Biotechnology, Vasyl Stefanyk Precarpathian National University, Ivano-Frankivsk, Ukraine;
2Laboratory of Experimental Toxicology and Mutagenesis, L. I. Medved’s Research Center of Preventive Toxicology, Food and Chemical Safety, MHU, Kyiv, Ukraine;
3Research and Development University, Ivano-Frankivsk, Ukraine;
*e-mail: olya_b08@ukr.net or oleh.lushchak@pnu.edu.ua

Received: 04 October 2021; Accepted: 21 January 2022

The influence of the developmental nutrition on adult metabolism and overall performance becomes a hot topic of modern evolutionary biology. We used fruit fly Drosophila melanogaster as a model and experimental nutrition media composed of different sucrose content (S) and dry yeast content (Y): 0S:2Y, 20S:2Y or 0S:5Y, 20S:5Y to show that the developmental nutrition conditions define metabolism in larvae and adults. The level of glucose, glycogen, triglycerids and total lipids in the larvae and flies body were measured with the diagnostic assay kits. We found that individuals developed on either low-yeast or high-sugar diet showed delayed developmental rate. When kept on the diets with high sucrose content the larvae and adult flies had lower weight and higher amount of lipids as energy reserves. Restriction of dry yeast content in the diet of larvae led to a decrease in glycogen storage and protein levels in larvae and adult flies. The results obtained indicate that the metabolic traits revealed in adult flies are the result of nutrition during development and may be associated with mechanisms of organisms adaptation to the developmental nutritional conditions.

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Fructose as a factor of carbonyl and oxidative stress development and accelerated aging in the yeast Saccharomyces cerevisiae

20.02.2018   Uncategorized   No comments

L. М. Lozinska, H. М. Semchyshyn

Vassyl Stefanyk Precarpathian National University, Ivano-Frankivsk, Ukraine;
е-mail: semchyshyn@pu.if.ua

Excessive and prolonged consumption of fructose may lead to the development of metabolic disorders. However, the mechanisms of  disturbances are still discussed. In the present work, the budding yeast Saccharomyces cerevisiae has been used as a model to compare the effects of prolonged consumption of different concentrations of glucose and fructose on certain physiology-biochemical parameters of eukaryotes. It has been shown that the yeast growth, their metabolic activity, intracellular level of glycogen and oxidized proteins were higher in cells grown on fructose. The observation is consistent with the data on a higher in vitro ability of fructose than glucose to initiate glycation which products of which are highly reactive α-dicarbonyl compounds and activated oxygen forms. Thus the intensity of carbonyl and oxidative stress is higher in cells grown on fructose. This can explain a higher rate of aging of yeast consuming fructose as a source of carbon and energy as compared to cells growing on glucose. However, carbohydrate restriction used in this study hampered the accumulation of glycogen and oxidized proteins and did not reveal any difference between markers of aging and carbonyl and oxidative stress in yeast grown on glucose and fructose.

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