Tag Archives: MDA
Physiological and biochemical parameters of winter wheat Triticum aestivum L. plants after seed treatment with fullerene C(60)
S. Prylutska1*, T. Tkachenko1, M. Petrovsky2
1National University of Life and Environmental Sciences of Ukraine, Kyiv;
2Taras Shevchenko National University of Kyiv, Ukraine.
*e-mail: psvit_1977@ukr.net
Received: 21 February 2025; Revised: 25 March 2025;
Accepted: 25 April 2025; Available on-line: 12 May 2025
Extreme climatic conditions, pests, diseases and environmental pollution significantly impact the cultivation of agricultural products and the quality of plant raw materials. It is assumed that nanostructured carbon materials, particularly fullerene C60, due to antioxidant, antiviral, and antibacterial properties can be used to prevent these effects. This study aimed to evaluate the effect of pre-sowing treatment of wheat seeds with fullerene C60 on the state of plants 14 days after germination. The seeds of the winter wheat Triticum aestivum L. of the Patras and Akter varieties were treated with a colloidal solution of fullerene C60 (0.1-1.0 µg/ml) for 3 h. Biomorphometric parameters, photosynthetic pigments, phenolic compounds, MDA content and catalase activity were assessed using standard techniques. It was shown that seeds treatment with fullerene C60 was followed by the greater increase of both the fresh weight of Akter plants and shoot length of Patras plants as compared to untreated controls. A dose-dependent effect of fullerene C60 on the physiological and biochemical parameters of the plants was revealed. Photosynthetic activity in plants of both wheat varieties was enhanced after seed treatment with C60 in low (0.1-0.2 µg/ml) concentrations as evidenced by the increased content of chlorophylls a, while at high (0.5-1.0 µg/ml) C60 concentrations it decreased against the background of increased carotenoids content. The enhancement of antioxidant defense induced by C60 treatment at concentrations of 0.5-1.0 µg/ml was observed, as indicated by an increase in the content of phenolic compounds and activation of catalase. The positive effect of wheat seeds treatment with fullerene C60 indicates the potential use of carbon nanoparticles in agrobiotechnologies to improve plant growth and stress resistance.
Lipid peroxidation and DNA fragmentation in fresh and cryopreserved spermatozoa of men at different spermatogenesis state
T. O. Yurchuk*, O. V. Pavlovich, G. O. Gapon,
A. Y. Pugovkin, M. P. Petrushko
Institute for Problems of Cryobiology and Cryomedicine, National Academy of Sciences of Ukraine, Department of Cryobiology of Reproductive System, Kharkiv;
*e-mail: taisiya.yur@gmail.com
Received: 11 July 2021; Accepted: 17 May 2021
Cryopreservation of spermatozoa is widely used in the treatment of infertility by assisted reproductive technologies. However, the cryopreservation causes an oxidative stress which can induce pathological changes in the male gametes. The aim of the research was to evaluate lipid peroxidation (LPO) and DNA fragmentation as well as correlation between these parameters in the fresh and cryopreserved spermatozoa of men with normozoospermia or oligoasthenoteratozoospermia (OAT) and spermatozoa derived from the epididymis of men with azoospermia. The level of malondialdehyde (MDA) in a TBA test, superoxide dismutase (SOD) activity and total antioxidant activity (AOA) were assessed. DNA integrity was estimated by acridine orange staining technique. It was shown that MDA level and SOD activity were significantly higher in the fresh spermatozoa of oligoasthenoteratozoospermic group compared with the fresh spermatozoa of normozoospermic group. After cryopreservation the MDA level increased in all groups and was the highest in OAT group where the greatest AOA decline was detected. DNA fragmentation frequency was 2.6 and 4.1 times higher in the fresh and cryopreserved OAT spermatozoa respectively as compared with the fresh normozoospermic ones (7.2%). DNA fragmentation was found to be the lowest in the fresh (6.2%) and cryopreserved (5.8%) epididymal spermatozoa. After cryopreservation SOD activity in epididymal spermatozoa was lower than in normozoaspermic. In spermatozoa of the studied groups the MDA level positively correlated with DNA fragmentation (0.79 Pearson’s correlation coefficient) both before and after cryopreservation. It is suggested that due to the detected low DNA fragmentation and LPO level in epididymal spermatozoa their use could be an alternative approach for infertility treatment by assisted reproductive technologies.