Tag Archives: metabolic syndrome
Extracellular matrix degradation products in the heart of rats with metabolic syndrome under chromium picolinate administration
O. Ye. Akimov1*, A. O. Mykytenko2, V. O. Kostenko1
1Department of Pathophysiology, Poltava State Medical University, Poltava, Ukraine;
*e-mail: o.akimov@pdmu.edu.ua;
2Department of Biological and Bioorganic Chemistry,
Poltava State Medical University, Poltava, Ukraine
Received: 22 May 2025; Revised: 24 July 2025;
Accepted: 12 September 2025; Available on-line: 17 September 2025
The populace of Ukraine shows a trend of increasing percentage of persons with obesity, complicated by metabolic syndrome (MetS), which causes damage to the heart extracellular matrix. According to recent studies chromium, picolinate (CrPIC) has the potential to attenuate lipid metabolism disorders and protect the extracellular matrix from degradation. The aim of this research was to estimate the blood lipid profile and the content of glycosaminoglycans, L-hydroxyproline and sialic acids in the heart of rats with simulated metabolic syndrome under Chromium picolinate administration. Mature male Wistar rats were divided into 4 groups of 6 animals each – control; metabolic syndrome induction; CrPIC administration; metabolic syndrome + CrPIC administration. Metabolic syndrome was reproduced by using a 20% fructose solution as the only source of water for 60 days. CrPIC was administered orally at a dose of 80 µg/kg daily for 60 days. The concentration of the heart extracellular matrix degradation proteins was determined spectrophotometrically in a 10% heart homogenate. CrPIC administration to healthy animals stimulated the accumulation of glycosaminoglycans chondroitin fraction in the rat heart. Metabolic syndrome modeling resulted in an increase in TG, TC and LDL-C blood levels, intensification of collagenolysis, degradation of glycoproteins and glycosaminoglycans with a predominance of the keratan-dermatan fraction. CrPIC administration to animals with metabolic syndrome reduced collagenolysis and glycoproteins degradation, changed the dominating fraction of glycosaminoglycans from keratan-dermatan to chondroitin in rat heart connective tissue indicating its potential to prevent cardiac tissue remodeling during metabolic syndrome.
Nitric oxide cycle activity in rat biceps femoris muscle under conditions of bacterial lipopolysaccharide influence, experimental metabolic syndrome and their combination
O. Ye. Akimov1*, A. O. Mykytenko2, V. O. Kostenko1
1Department of Pathophysiology, Poltava State Medical University, Poltava, Ukraine;
*e-mail: o.akimov@pdmu.edu.ua;
2Department of Biological and Bioorganic Chemistry, Poltava State Medical University, Poltava, Ukraine
Received: 22 April 2023; Revised: 05 June 2023;
Accepted: 7 September 2023; Available on-line: 12 September 2023
There is evidence that long-term organism stimulation with bacterial lipopolysaccharides (LPS), which promotes the secretion of pro-inflammatory cytokines and nitric oxide, may play an important role in metabolic syndrome (MetS) development. Changes in NO production under conditions of MetS have different directions and depend on a specific organ. The purpose of this work was to study the production of nitric oxide and its metabolites in the biceps femoris muscle of rats under conditions of lipopolysaccharide stimulation of the organism, metabolic syndrome and their combination. The study was conducted for 60 days on 24 male Wistar rats divided into control, MetS, LPS and LPS+MetS groups. MetS was reproduced by adding 20% fructose solution to food, LPS stimulation was carried out by intraperitoneal injection of S. typhi LPS. It was demonstrated that stimulation of the rat organism with LPS under conditions of experimental metabolic syndrome increased the production of nitric oxide by L-arginine-dependent pathway, but limited metabolic syndrome-induced increase in nitric oxide production by L-arginine-independent pathway, reduced the concentration of S-nitrosothiols, while increasing the concentration of peroxynitrites and nitrites in the biceps femoris muscle of rats.
Indicators of lipid metabolism disorders in the blood serum of adolescents with metabolic syndrome
L. A. Strashok1,2, O. V. Buznytska1,2*, О. М. Meshkova3
1Kharkіv Medical Academy of Postgraduate Education, Ukraine;
2V.N. Karazin Kharkіv National University, Ukraine;
3Bogomolets National Medical University, Kyiv, Ukraine;
*e-mail: ebuznickaa@ukr.net; elena.buznytska@gmail.com
Received: 16 April 2018; Accepted:13 November 2020
Atherogenic dyslipidemia is one of the main and early indication of adolescentrs with metabolic syndrome. Because there is not enough information about the nature of dyslipidemia in adolescents and the possibilities of its correction and prevention, the study of this problem is relevant. The goal of the study was to estimate the indexes of lipid metabolism in blood serum of adolescents with metabolic syndrome. We examined 200 obese patients aged 14–18 years, who were divided into two groups with and without metabolic syndrome. The control group consisted of 30 adolescents of similar age with normal body weight. The lipid profile of blood was studied by standardized methods using Cormay Multi semi-automatic photometer. The level of β-lipoproteins in blood serum was determined by the turbidimetric method. Elevated levels of triglycerides, low and very low density lipoprotein cholesterol, β-lipoproteins and a tendency to decreased high density lipoprotein level cholesterol were found in the blood serum of adolescents with metabolic syndrome as the signs of atherogenic dyslipidemia. Reliable correlations both between the indexes of lipid profile and between atherogenic dyslipidemia indicators and anthropometric indexes were established, indicating an increased risk of lipid metabolic disturbances in individuals with abdominal obesity.