Tag Archives: nitrates

Effect of nitrates on the bone marrow of rats

M. V. Danchyshyn*, O. V. Lototska

I. Horbachevsky Ternopil National Medical University, Ukraine;
*e-mail: danchyshyn@tdmu.edu.ua

Received: 28 January 2022; Revised: 21 March 2022;
Accepted: 20 September 2022; Available on-line: 19 December 2022

Nitrate pollution of groundwater has been increasing in recent years. The aim of this work was to study the morphological features of the bone marrow of rats at the consumption of drinking water with varying nitrate content. The experiment was performed on 2 groups of white female rats of different ages (mature and immature) with 30 individuals each. Both groups were divided into 5 subgroups that differed in the quality of drinking water consumed by rats on their own. In the 30 days study, nitrate was given to rats in drinking water in doses of 50, 150, 250, 500 mg of sodium nitrate per liter. Animals of the control group drank water from the city water supply. The effect of nitrates on the bone marrow of rats was evaluated by morphological analysis. It was found that the consumption of drinking water with nitrates affected erythropoiesis and leukopoiesis, as evidenced by the growth of blasts, lymphocytes, pronormocytes and normocytes, megakaryocytes and eosinophils, displacement of erythrocyte sprout cells. An increase in the neutrophil maturation index indicates delayed neutrophil maturation, increased yield of mature cells from the bone marrow and depletion of the granulocyte lineage. The more pronounced changes were observed in the qualitative and quantitative composition of the components of the erythrocyte sprout. These disorders were dose-dependent with increasing hematotoxic effect of nitrates at doses of 250 and 500 mg/l in the group of immature animals and resulted in changes in the bone marrow, which may further cause the development of pathological processes in an organism.

The effect of natural polyphenol complex of red grape wine ON L-arginine/NO system in peripheral blood of rats under low doses of ionizing radiation

M. V. Sabadashka1, A. R. Gnatush1, L. O. Datsyuk1, U. V. Staranko1, A. N. Fedorovych1,
V. G. Herzhykova2, A. M. Zotov2, E. A. Slastya2, N. O. Sybirna1

1Ivan Franko National University of Lviv, Ukraine;
е-mail: sybirna_natalia@yahoo.com;
2National Institute for Vine and Wine «Magarach», Crimea, Ukraine;
е-mail: magarach@rambler.ru

The total activity of NO-synthase and content of stable metabolitic products of nitric oxide in the peripheral blood of rats under low doses of ionizing radiation and administration of natural polyphenol complex of grape was investigated. It was found that natural polyphenol compounds of grapes have the ability to correct radioinduced changes in L-arginine/NO system. It was noted that the action of X-radiation increased activity of NO-synthase in the peripheral blood of rats. However, the consumption of natural polyphenol complex of grape led to a decrease of this index to control values.
An increase in NOS activity under irradiation leads to the increase of NO stable metabolites content, which is reflected in the accumulation of nitrite- and nitrate-anions in the peripheral blood of rats. The consumption of preparation of polyphenol complex this index decreased in the early period of the experiment, and on the third day after exposure, the total content of NO stable metabolites is slightly higher compared to the indices of control animals. Thus, the ability of natural polyphenol complex to cause attenuation of oxidative-nitrative stress caused by ionizing radiation was investigated experimentally.