Tag Archives: osteoarthritis
Osteopontin as a biomarker for diagnosing the severity of osteoarthritis
M. A. Taha, Z. M. A. A. Hamodat*
Department of Chemistry, College of Science, University of Mosul, Iraq;
*e-mail: zahraahamodat@uomosul.edu.iq
Received: 25 April 2024; Revised: 30 August 2024;
Accepted: 07 October 2024; Available on-line: 28 October 2024
Osteoarthritis (OA) is the most common type of arthritis. It is characterized by the loss of cartilage and pain ultimately leading to difficulty in movement. Glycoprotein osteopontin (OPN) is an essential regulator that plays a critical role in developing osteoarthritis. The study aims to determine the level of OPN in the serum of patients with OA and its correlation with the content of calcium (Ca), phosphorus (P), and magnesium (Mg). The study was conducted on 92 male and female patients aged between 30 and 65, categorized into mild, moderate, and severe groups through clinical examination and X-rays. The control group consist of 58 healthy males and females 30-65-year old. OPN was measured using the ELISA technique, and minerals were estimated using spectrophotometry. It was shown that in OA patients, OPN level increased highly significant when compared with the control group (10.7 ± 3.4 ng/ml) and depended on OA severity. In patients with severe OA, it was higher (47.5 ± 8.1 ng/ml) than in groups with moderate (14.8 ± 4.5 ng/ml) and mild (12.1 ± 3.1 ng/ml) extent of the disease. We showed a significant positive relationship between OPN and phosphorus levels and a negative significant correlation between OPN and calcium, calcium/phosphorus ratio, and magnesium. These findings underscore the potential of OPN as a valuable biomarker for diagnosing the severity of osteoarthritis and monitoring the effectiveness of treatment.
Interleukin-8 level in the blood of rats with experimental osteoarthritis and hypothyroidism
D. S. Nosivets
SI “Dnipropetrovsk Medical Academy of the Ministry of Health of Ukraine”;
e-mail: dsnosivets@ukr.net
Received: 9 May 2020; Accepted: 13 November 2020
In the study, the level of interleukin-8 in the blood serum of rats with experimental model of osteoarthritis and hypothyroidism under the influence of NSAID s and paracetamol was estimated. The experiments were performed on 75 white nonlinear rats of both sexes. Experimental osteoarthritis was induced by single intra-articular administration of 0.1 ml of monoiodoacetic acid solution into the knee joint. Experimental hypothyroidism was reconstructed by enteral administration of 0.02% carbimazole solution, given with a drinking ration for 6 weeks. On the 42nd day of the experiment, the animals were divided into 14 groups and drug administration began daily for 5 days. The serum level of interleukin-8 was estimated on 42 and 47 days of the experiment by specific ELISA kits. A significant decrease (P ≤ 0.05) of the elevated cytokine level under the influence of pharmacotherapy was revealed. According to the degree of influence on degenerative-dystrophic process in the knee joint, the investigated drugs can be arranged as follows: diclofenac sodium > ibuprofen > nimesulide = meloxicam > celecoxib > paracetamol. The results obtained showed that the level of interleukin-8 could be used as an indicator of non-steroidal drugs and paracetamol anti-inflammatory activity under experimental osteoarthritis and hypothyroidism.