Tag Archives: osteoarthritis

Interleukin-8 level in the blood of rats with experimental osteoarthritis and hypothyroidism

D. S. Nosivets

SI “Dnipropetrovsk Medical Academy of the Ministry of Health of Ukraine”;
e-mail: dsnosivets@ukr.net

Received: 9 May 2020; Accepted: 13 November 2020

In the study, the level of interleukin-8 in the blood serum of rats with experimental model of osteoarthritis and hypothyroidism under the influence of NSAID s and paracetamol was estimated. The experiments were performed on 75 white nonlinear rats of both sexes. Experimental osteoarthritis was induced by single intra-articular administration of 0.1 ml of monoiodoacetic acid solution into the knee joint. Experimental hypothyroidism was reconstructed by enteral administration of 0.02% carbimazole solution, given with a drinking­ ration for 6 weeks. On the 42nd day of the experiment, the animals were divided into 14 groups and drug administration began daily for 5 days. The serum level of interleukin-8 was estimated on 42 and 47 days of the experiment by specific ELISA kits. A significant decrease (P ≤ 0.05) of the elevated cytokine level under the influence of pharmacotherapy was revealed. According to the degree of influence on degenerative-dystrophic process in the knee joint, the investigated drugs can be arranged as follows: diclofenac sodium > ibuprofen > nimesulide = meloxicam > celecoxib > paracetamol. The results obtained showed that the level of interleukin-8 could be used as an indicator  of  non-steroidal drugs and paracetamol anti-inflammatory activity under  experimental osteoarthritis and hypothyroidism.

Oxidative/antioxidant balance and matrix metalloproteinases level in the knee cartilage of rats under experimental osteoarthritis and probiotic administration

O. Korotkyi*, K. Dvorshchenko, L. Kot,
T. Vovk, M. Tymoshenko, L. Ostapchenko

ESC “Institute of Biology and Medicine”,
Taras Shevchenko National University of Kyiv, Ukraine;
*e-mail: korotkyi@gmail.com

Received: 28 June 2020; Accepted: 13 November 2020

The aim of this work was to investigate the effect of the poly-strain probiotic on oxidative-antioxidant balance and the level of matrix metalloproteinases (MMPs) in rat knee cartilage under experimental osteoarthritis. Osteoarthritis was induced by a single injection of monoiodoacetate into the knee joint of rats. Probiotic was administered daily for 14 days. Knee cartilages homogenate was used to evaluate  the content of reactive oxygen species (superoxide anion and hydrogen peroxide), products of lipids peroxidation (diene conjugates, TBA-active compound, Shiff bases), to determine superoxide dismutase and catalase activity and activity of glutathione-dependent  antioxidant enzymes, the level of reduced and oxidized glutathione. The level of MMPs -1, -2, -3, -8 expression was estimated by ELISA. Osteoarthritis was found to cause a significant increase in the reactive oxygen species level, lipid peroxidation products content, superoxide dismutase and catalase activity, level of all studied MMPs, and also depletion of glutathione-dependent antioxidant system and the decrease in the ratio between reduced and oxidized glutathione.The administration of the probiotic was followed by the tendency for the restoration of the parameters to the values of the control group. Thus, the administration of the probiotic to rats with osteoarthritis may be considered as an anti-inflammatory and antioxidant agent for further clinical trials.