Tag Archives: polyamines

Hydrogen peroxide as a signal mediator at inducing heat resistance in wheat seedlings by putrescine

Yu. E. Kolupaev1,2, A. I. Kokorev1, T. O. Yastreb1, E. I. Horielova1

1Dokuchaev Kharkiv National Agrarian University, Ukraine,
e-mail: plant_biology@ukr.net;
2Karazin Kharkiv National University, Ukraine

Received: 27 May 2019; Accepted: 18 October 2019

Polyamines are multifunctional stress metabolites of plants. However, information on the effect of exo­genous polyamines on plant resistance to high temperatures is contradictory, and it remains unclear which signal mediators are involved in the realization of their physiological effects. The possible involvement of hydrogen peroxide as a mediator under the action of exogenous diamine putrescine on the resistance of etiolated wheat seedlings (Triticum aestivum L.) to hyperthermia (10-minute heating at 46°C) and the functioning of antioxidant system was investigated. It was established that the treatment of seedlings with putrescine in 0.25–2.5 mM concentrations caused a significant increase in their heat resistance. In response to the putrescine effect, a transient increase in the H2O2 content occurred in the root cells. This effect was eliminated by treatment of seedlings with a diamine oxidase inhibitor aminoguanidine and an NADPH oxidase inhibitor imidazole. These inhibitors, as well as the scavenger of hydrogen peroxide dimethylthiourea (DMTU), mitigated the effects of increased heat resistance of seedlings and increased activity of superoxide dismutase and catalase caused by putrescine. Under the influence of DMTU and imidazole, but not aminoguanidine, the effect of increasing the activity of guaiacol peroxidase in the roots of seedlings treated with putrescine was eliminated. The conclusion was made about the role of hydrogen peroxide and the possible participation of diamine oxidase and NADPH oxidase in its formation during the implementation of the stress-protective effect of putrescine on wheat seedlings.

Amine oxidases as important agents of pathological processes of rhabdomyolysis in rats

O. O. Gudkova, N. V. Latyshko, S. G. Shandrenko

Palladin Institute of Biochemistry, National Academy of Sciences of Ukraine, Kyiv;
e-mail: ogudkova@biochem.kiev.ua

In this study we have tested an idea on the important role of amine oxidases (semicarbazide-sensitive amine oxidase, diamine oxidase, polyamine oxidase) as an additional source of oxidative/carbonyl stress under glycerol-induced rhabdomyolysis, since the enhanced formation of reactive oxygen species and reactive carbonyl species in a variety of tissues is linked to various diseases. In our experiments we used the sensitive fluorescent method devised for estimation of amine oxidases activity in the rat kidney and thymus as targeted organs under rhabdomyolysis. We have found in vivo the multiple rises in activity of semicarbazide-sensitive amine oxidase, diamine oxidase, polyamine oxidase (2-4.5 times) in the corresponding cell fractions, whole cells or their lysates at the 3-6th day after glycerol injection. Aberrant antioxidant activities depended on rhabdomyolysis stage and had organ specificity. Additional treatment of animals with metal chelator ‘Unithiol’ adjusted only the activity of antioxidant enzymes but not amine oxidases in both organs. Furthermore the in vitro experiment showed that Fenton reaction (hydrogen peroxide in the presence of iron) products alone had no effect on semicarbazide-sensitive amine oxidase activity in rat liver cell fraction whereas supplementation with methylglyoxal resulted in its significant 2.5-fold enhancement. Combined action of the both agents had additive effect on semicarbazide-sensitive amine oxidase activity. We can assume that biogenic amine and polyamine catabolism by amine oxidases is upregulated by oxidative and carbonyl stress factors directly under rhabdomyolysis progression, and the increase in catabolic products concentration contributes to tissue damage in glycerol-induced acute renal failure and apoptosis stimulation in thymus.

Laureates of the Palladin Prize of the National Academy of Sciences of Ukraine (1989–1990)

R. P. Vynogradova, V. M. Danilova

Palladin Institute of Biochemistry, National Academy of Sciences of Ukraine, Kyiv;
e-mail: valdan@biochem.kiev.ua

In 1989 N. K. Berdinskikh and S. P. Zaletok received the Palladin Prize of NAS of Ukraine for the monograph “Polyamines and tumor growth” and in1990 A. M. Belous, V. A. Bondarenko and O. K. Gulevsky were awarded the prize for the series of works “Studies of the mechanisms of cryoinjury of biological membranes” and the monograph “Biomembrane barrier properties at low temperatures”.