Tag Archives: SOD

Expression of antioxidant enzymes genes in the liver and cardiac tissues of rats under L-carnitine administration and high-intensity interval exercise training

B. Shahouzehi1,2, Y. Masoumi-Ardakani3, S. Aminizadeh3, H. Nasri2*

1Student Research Committee, Kerman University of Medical Sciences, Kerman, Iran;
2Cardiovascular Research Center, Institute of Basic and Clinical Physiology Sciences, Kerman University of Medical Sciences, Kerman, Iran;
3Physiology Research Center, Institute of Neuropharmacology, Kerman University of Medical Sciences, Kerman, Iran;
*e-mail: dr_hrnasri@yahoo.com
 
Received: 29 September 2020; Accepted: 07 July 2021

Reactive oxygen and nitrogen species are produced in the body both in normal and pathological processes and can alter cell redox and affect cell functions. Exercise training is able to modulate oxidant/antioxidants balance. In this study, we aimed to evaluate expression of antioxidant enzymes genes in the liver and cardiac tissues of rats that performed high-intensity interval training (HIIT) and received L-carnitine (LCAR). Thirty-two male Wistar rats were were randomly assigned into 4 groups (n = 8) as follows: 1. Untreated control; 2. The group that received LCAR (200 mg/kg/day i.p.); 3. The group that performed HIIT on a readmill (5 days/week for 4 weeks); 4. The group  that received LCAR and performed HIIT. At the end of the study, liver and cardiac tissues were excised and used to quantify glutathione peroxidase (GPX), superoxide dismutase (SOD), catalase (CAT) and NF-κB genes expression by real-time PCR. It was found that both in LCAR and  HIIT groups GPX, SOD and NF-κB (P < 0.01) expression in cardiac and liver tissues was  significantly increased compared to the indices in the control group. In LCAR-HIIT group SOD and NF-κB expression in the liver was significantly increased compared to the group that received LCAR only (P = 0.046).  Our results showed that LCAR supplementation is useful to improve oxidative status in cardiac and liver tissues of rat during exercise training.

Lipid peroxidation and DNA fragmentation in fresh and cryopreserved spermatozoa of men at different spermatogenesis state

T. O. Yurchuk*, O. V. Pavlovich, G. O. Gapon,
A. Y. Pugovkin, M. P. Petrushko

Institute for Problems of Cryobiology and Cryomedicine, National Academy of Sciences of Ukraine, Department of Cryobiology of Reproductive System, Kharkiv;
*e-mail: taisiya.yur@gmail.com

Received: 11 July 2021; Accepted: 17 May 2021

Cryopreservation of spermatozoa is widely used in the treatment of infertility by assisted reproductive technologies. However, the cryopreservation causes an oxidative stress which can induce pathological changes in the male gametes. The aim of the research was to evaluate lipid peroxidation (LPO) and DNA fragmentation as well as correlation between these parameters in the fresh and cryopreserved spermatozoa of men with normozoospermia or oligoasthenoteratozoospermia (OAT) and spermatozoa derived from the epididymis of men with azoospermia. The level of malondialdehyde (MDA) in a TBA test, superoxide dismutase (SOD) activity and total antioxidant activity (AOA) were assessed. DNA integrity was estimated by acridine orange staining technique. It was shown that MDA level and SOD activity were significantly higher in the fresh spermatozoa of oligoasthenoteratozoospermic group compared with the fresh spermatozoa of normozoospermic group. After cryopreservation the MDA level increased in all groups and was the highest in OAT group where the greatest AOA decline was detected. DNA fragmentation frequency was 2.6 and 4.1 times higher in the fresh and cryopreserved OAT spermatozoa respectively as compared with the fresh normozoospermic ones (7.2%). DNA fragmentation was found to be the lowest in the fresh (6.2%) and cryopreserved (5.8%) epididymal spermatozoa. After cryopreservation SOD activity in epididymal spermatozoa was lower than in  normozoaspermic. In spermatozoa of the studied groups the MDA level positively correlated with DNA fragmentation (0.79 Pearson’s correlation coefficient) both before and after cryopreservation. It is suggested that due to the detected low DNA fragmentation and LPO level in epididymal spermatozoa their use could be an alternative approach for infertility treatment by assisted reproductive technologies.